Method for measuring content of luteolin in callicarpa nudiflora preparation

A technology of luteolin and luteolin, which is applied in the direction of anti-inflammatory agents, anti-virus agents, anti-viral agents, etc., can solve the problems of no method for measuring luteolin content and inaccurate quantification, and achieve small errors and ensure clinical effect, effect of raising quality standards

Active Publication Date: 2011-10-05
JIANGXI POZIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly granules, capsules, tablets, dispersible tablets, mixtures, soft capsules, suppositories, etc. in the market. The quality standards of the above-mentioned preparations are only part of the preparations. The content of total flavonoids has been determined by ultraviolet spectroscopy Photometric method, the quantification is very inaccurate, there is no method for the content determination of luteolin in the nude flower purple pearl preparation

Method used

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  • Method for measuring content of luteolin in callicarpa nudiflora preparation
  • Method for measuring content of luteolin in callicarpa nudiflora preparation
  • Method for measuring content of luteolin in callicarpa nudiflora preparation

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Embodiment 1

[0060] The determination method of luteolin content in the nude flower purple pearl preparation, adopts high performance liquid chromatography to measure the luteolin content in the nude flower purple pearl preparation test solution.

[0061] Chromatographic conditions and system applicability Octadecylsilane bonded silica gel was used as filler, methanol-0.1% phosphoric acid solution (60:40) was used as mobile phase, the detection wavelength was 348nm, and the number of theoretical plates should be calculated based on the peak of luteolin. Below 3000.

[0062] Preparation of reference substance solution Take an appropriate amount of luteolin reference substance, weigh it, add 70% methanol to make a solution containing 30mg per 1ml, and get it.

[0063] Preparation of the test solution Take 2 bags of nude flower purple pearl granules, grind finely, take 2g, weigh it, put it in a 100mL conical flask with a stopper, add 25mL of 70% ethanol, weigh it, ultrasonic for 30 minutes, l...

Embodiment 2

[0067] The determination method of luteolin content in the nude flower purple pearl preparation, adopts high performance liquid chromatography to measure the luteolin content in the nude flower purple pearl preparation test solution.

[0068] Chromatographic conditions and system applicability Octadecylsilane bonded silica gel is used as filler, acetonitrile-0.1% acetic acid solution (62:38) is used as mobile phase, the detection wavelength is 340nm, and the number of theoretical plates should be calculated according to the peak of luteolin Below 3000.

[0069] Preparation of reference substance solution Take an appropriate amount of luteolin reference substance, weigh it, add 70% methanol to make a solution containing 30mg per 1ml, and get it.

[0070] Preparation of the test solution Take 2 bags of nude flower purple beads, grind finely, take 2.2g, weigh it, put it in a 100mL conical flask with a stopper, add 25mL of 70% ethanol, weigh it, ultrasonic for 30 minutes, let it c...

Embodiment 3

[0074] The determination method of luteolin content in the nude flower purple pearl preparation, adopts high performance liquid chromatography to measure the luteolin content in the nude flower purple pearl preparation test solution.

[0075] Chromatographic conditions and system applicability Octadecylsilane bonded silica gel is used as filler, methanol-0.3% phosphoric acid solution (50:50) is used as mobile phase, the detection wavelength is 355nm, and the number of theoretical plates should be calculated based on the peak of luteolin. Below 3000.

[0076] Preparation of reference substance solution Take an appropriate amount of luteolin reference substance, weigh it, add 70% methanol to make a solution containing 30mg per 1ml, and get it.

[0077] Preparation of the test solution Take 10 pieces of Nudehuazizhu Tablets, grind finely, take 1.3g, weigh it, put it in a 100mL conical flask with a stopper, add 50mL of 50% ethanol, weigh it, ultrasonic for 45 minutes, let it cool ...

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Abstract

The invention discloses a method for measuring the content of luteolin in a callicarpa nudiflora preparation, relating to a method for measuring the content of effective ingredients in the callicarpa nudiflora preparation. A high performance liquid chromatography (HPLC) is used for measuring the content of luteolin in the test article solution of the callicarpa nudiflora preparation. Chromatographic conditions and system suitability are as follows: octadecylsilane chemically bonded silica is used as a filler, a flowing phase contains an organic solvent and an acidic solution, the weight percent of the organic solvent in the flowing phase is 50-70%, the weight percent of the acidic solution in the flowing phase is 30-50%, the concentration of the acidic solution is 0-0.3%, and the detection wavelength is 340-355nm. The method disclosed by the invention can be used for accurately and effectively measuring the content of luteolin in the callicarpa nudiflora preparation, has a small error, can further improve the quality standard of the callicarpa nudiflora preparation, and can ensure the quality and clinical effect of the callicarpa nudiflora preparation.

Description

technical field [0001] The invention relates to a method for determining the content of active ingredients in a nudibranch preparation, in particular to a method for determining the content of luteolin in a nude flower preparation. Background technique [0002] Callicarpanudiflorae is a small shrub plant of Verbenaceae, which has the functions of hemostasis, antibacterial, astringent, detoxification, analgesia, and promotion of healing. Nadihuazizhu can also inhibit the permeability of capillaries, and has obvious anti-inflammatory effect on early inflammation exudation and swelling. At the same time, the naked flower purple pearl also has the function of protecting the liver and reducing enzymes, and can be used to treat viral hepatitis. The main components of Nadiflower are flavonoid glycosides, condensed tannins, neutral resins, phenols, polysaccharides, hydroxyl compounds, magnesium, calcium, iron salts, etc. Among them, shazurpenone, apigenin, verbascoside, and mignone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/85G01N30/02G01N30/06A61P7/04A61P31/04A61P39/02A61P29/00A61P9/14A61P17/02A61P31/20A61P1/16A61P11/00A61P31/14A61K31/352
Inventor 龚志南吴永忠余宝平余显英李旭张荷英
Owner JIANGXI POZIN PHARMA
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