Process for preparing verbascoside

A technology of verbascoside and process, which is applied in the field of preparing verbascoside, can solve the problems of inconvenient continuous operation, low yield, poor product quality, etc., and achieve the effects of short production cycle, high extraction rate and good effect

Inactive Publication Date: 2010-10-20
NANJING ZELANG AGRI DEV
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  • Abstract
  • Description
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Problems solved by technology

Chinese invention patent application 99123015.9 discloses "a method for extracting verbascoside", using ethanol extraction, dissolving in hot water, sequentially extracting with petroleum ether, ethyl acetate and n-butanol, dissolving in methanol, and extracting verbascoside by silica gel column chromatography , but the yield of this method is low, the product quality is poor, and it is not convenient for continuous operation

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  • Process for preparing verbascoside

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Effect test

Embodiment 1

[0031] Take 20kg of large plantain seed (8.6mg / g), put it into an extraction tank, add 300L saturated lime water and soak at 60°C for 1.5 hours, then ultrasonically extract for 1 hour, filter, extract twice, adjust the pH to 7 with hydrochloric acid solution, and obtain Add 2.9kg of activated carbon to 580L extract, stir for half an hour, filter, then add 2 times the amount of petroleum ether to degrease. The degreased liquid is first processed through the microfiltration membrane system, and then passed through the ultrafiltration membrane system to collect the permeate, add the permeate to D101 macroporous resin for adsorption, first use 6 times the column volume of deionized water to elute impurities, and then use 5 times the column volume of n-butanol for elution, collect the eluent, recover the solvent, concentrate it into an extract, mix the extract with an equal amount of polyamide and put the sample on the column, first use 2 times the column volume of ethyl acetate / met...

Embodiment 2

[0033] Pulverize Budisma japonica (2%), put 10kg into the extraction tank, add 120L of 2% sodium carbonate solution and soak at 40°C for 2 hours, then ultrasonically extract for 1 hour, filter, extract twice, adjust the pH to 8 with hydrochloric acid solution, and obtain 200L of extract, add 1.5kg of activated carbon and stir for 1 hour, filter, then add double amount of 1 petroleum ether to degrease. The degreased liquid is first processed through the microfiltration membrane system, and then passed through the ultrafiltration membrane system to collect the permeate, add the permeate to HPD100 macroporous resin for adsorption, first use 5 times the column volume of deionized water to elute and remove impurities, and then use 6 times the column volume of n-butanol for elution, collect the eluent, recycle the solvent, concentrate it into an extract, mix the extract with an equal amount of polyamide and put the sample on the column, first use 3 times the column volume ethyl aceta...

Embodiment 3

[0035] Grind Artemisia annua (1.4%), put 10kg into the extraction tank, add 180L saturated lime water to soak for 3 hours at 50°C, then ultrasonically extract for 0.5 hour, filter, extract twice, adjust the pH to 7 with hydrochloric acid solution, and obtain 340L Add 3.4kg of active carbon to the extract, stir for 0.5 hour, filter, then add 3 times the amount of petroleum ether to degrease. The degreased liquid is first processed through the microfiltration membrane system, and then passed through the ultrafiltration membrane system to collect the permeate, add the permeate to AB-8 macroporous resin for adsorption, and first use 4 times the column volume of deionized water to elute and remove impurities. Use 7 times the column volume of n-butanol to elute, collect the eluate, recover the solvent, concentrate it into an extract, mix the extract with an equal amount of polyamide and put it on the column, first use 2 times the column volume ethyl acetate / methanol ( 9:1) and 3 tim...

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Abstract

The invention relates to a process for preparing verbascoside. In the process, plantain seed, butterflybush flower or pedicularisL plants serving as raw materials are crushed and soaked in alkaline solution for extraction for 1.5 to 3 hours; and the extract is decolorized and is separated and purified by a resin column, the purified extract is concentrated, and the concentrate is dried to obtain the product. The process, when used for producing the product, has the advantages of simple operation, repeated use of equipment, low operation cost, high product quality and the like.

Description

Technical field: [0001] The invention relates to a process for preparing verbascoside, in particular to a method for preparing verbascoside through ultrasonic-assisted alkali extraction, macroporous resin and polyamide purification, concentration and drying. Background technique: [0002] Verbacoside is a n-dihydroxycinnamic acid derivative of phenylpropanoid, also known as kusaside. [0003] Molecular formula: C 29 h 36 o 15 ;Molecular weight: 624.59; [0004] English name: Verbascoside [0005] Chemical name: 2-(3',4'-dihydroxyphenyl)ethyl-O-α-L-rhamnopyranosyl (1→3)-β-D-(4-O-caffeoyl) Glucopyranosides. [0006] [0007] Verbacoside is widely distributed in plants, and pharmacological studies have shown that verbascoside can keep MPP + Induced apoptosis of SHSY5Y cells has scavenging and inhibitory effects on oxygen free radicals and lipid peroxides in skeletal muscle, and has a delaying effect on muscle fatigue. Chinese invention patent application 03114454.3 di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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