Extract containing galloyl petogenin and its preparation method and application

A technology of galloyl group and galloyl rock, which is applied to the field of extracts containing galloyl petrolatum and its preparation field, can solve the problems of difficult industrialized production, complicated process, and has not yet been seen, and achieves easy industrialization and good whitening. effect, low cost

Active Publication Date: 2018-03-09
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method can obtain 11-O-(3',4',5'-trihydroxygalloyl)-peticinin monomer, it needs to use silica gel, liquid preparation and other means, the process is relatively complicated and the cost is high , not easy for industrial production
In addition, there are no related reports on the activity of extracts containing 11-O-(3',4',5'-trihydroxygalloyl)-petogenin

Method used

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  • Extract containing galloyl petogenin and its preparation method and application
  • Extract containing galloyl petogenin and its preparation method and application
  • Extract containing galloyl petogenin and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Take Sifang Rattan (11-O-(3',4',5'-trihydroxygalloyl)-petogenin content in the raw material is 1.0%), cut into slices, add 45 % ethanol, heated to reflux for 2h, filtered, collected the filtrate, repeated extraction of the filter residue once under the same conditions, filtered, combined the filtrates to obtain the extract;

[0029] 2) The obtained extract is concentrated to extract (8 baume), then applied to a HP20SS macroporous resin column, washed with pure water until the effluent is clear, then eluted with 15% ethanol of 2 times the volume of the column bed, and then washed with 45% ethanol Ethanol elution, thin-layer chromatography tracking detection (developing agent conditions: chloroform: methanol: water = 10:5:1, sulfuric acid ethanol heating for color development), collect 45% ethanol eluate containing the target component;

[0030] 3) The obtained alcohol eluate is concentrated under reduced pressure to recover ethanol, and then the effluent is collected ...

Embodiment 2

[0042] 1) Take 10kg of Sifang rattan (the content of 11-O-(3',4',5'-trihydroxygalloyl)-petogenin in the raw material is 1.0%), slice it, add 60 % ethanol, heat and reflux for extraction for 3 hours, filter, and collect the filtrate, which is the extract;

[0043] 2) The resulting extract is concentrated to extract (9 Baume), then put on the XDA-8 macroporous resin column, wash with pure water until the effluent is clear, then elute with 20% ethanol of 2 times the volume of the column bed, and then use 40% ethanol elution, TLC follow-up detection, collect 40% ethanol eluate containing target components;

[0044] 3) The obtained alcohol eluate is concentrated under reduced pressure to recover ethanol, then passed through a D941 decolorizing resin column, the effluent is collected, concentrated, and dried to obtain a crude extract; the obtained crude extract is crystallized and recrystallized with 95% ethanol respectively to obtain Extract containing 11-O-(3',4',5'-trihydroxygal...

Embodiment 3

[0047] 1) Take 10kg of vine (the content of 11-O-(3',4',5'-trihydroxygalloyl)-petogenin in the raw material is 1.0%), and add 90% ethanol equivalent to 6 times the weight of the raw material , heated to reflux for extraction for 1 h, filtered, collected the filtrate, repeated extraction of the filter residue once under the same conditions, filtered, and combined the filtrates to obtain the extract;

[0048] 2) The obtained extract is concentrated to extract (10 Baume), then put on the XDA-8 macroporous resin column, wash with pure water until the effluent is clear, then elute with 10% ethanol of 3 times the volume of the column bed, and then use 50 % ethanol elution, TLC tracking detection, collecting 50% ethanol eluate containing target components;

[0049] 3) The obtained alcohol eluate is concentrated under reduced pressure to recover ethanol, then passed through the LSA-700A decolorizing resin column, the effluent is collected, concentrated, and dried to obtain a crude ext...

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Abstract

The invention discloses an extract containing galloyl petracenin, a preparation method and application thereof. The preparation method comprises: 1) extracting the raw material with water or ethanol to obtain an extract; 2) subjecting the obtained extract to HP20SS or XDA-8 macroporous resin column chromatography after being concentrated, and washing with 10-20v / v% ethanol, then elute with 40-60v / v% ethanol, and collect the high-concentration alcohol eluate; 3) recover ethanol from the obtained alcohol eluate, decolorize, and then crystallize and recrystallize to obtain the product. In the present invention, the extract is chromatographed with a specific macroporous resin column to obtain a better purification and separation effect. After decolorization and impurity removal, the eluate can be crystallized to obtain an extract with a purity ≥ 60%, and the silica gel column is omitted. Chromatography and liquid preparation are operated, the process is simpler and easier to operate, the cost is lower, and it is easier to industrialize. The obtained extract has better whitening effect and stronger antioxidant effect.

Description

technical field [0001] The invention relates to the extraction of active ingredients in plants, in particular to an extract containing galloyl petrogin and its preparation method and application. Background technique [0002] The structure of 11-O-(3',4',5'-trihydroxygalloyl)-petogenin (11-O-galloylbergenin) is shown in the following formula: [0003] [0004] In the prior art, Zhang Wei et al. extracted and characterized 11-O-(3',4',5'-trihydroxygalloyl)-petogenin from the two-color Zijinniu (two-color Zijinniu Research on the chemical composition of cattle, Chinese Journal of Pharmaceutical Sciences, Volume 42, No. 9, May 2007, 656-657), specifically, the extract obtained from the two-color Zijinniu is extracted with petroleum ether and ethyl acetate sequentially, and ethyl acetate Part of the extract was chromatographed on a polyamide column, followed by gradient elution with water, volume fraction of 30%, 50%, and 70% ethanol, and 30% ethanol fractions were collected...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K8/49A61K31/366A61Q19/02A61Q19/08A61P39/06
Inventor 邱丽凰杨文国李元元李杰罗勇为
Owner GUILIN NATURAL INGREDIENTS CORP
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