Preparation method of cistanche deserticola phenylethanoid glycosides

A technology of phenylethyl alcohol total glycosides and cistanche, which is applied in food, health food processing applications, and pharmaceutical fields, and can solve problems such as easy oxidation of orthodiphenol groups, deepening of product color, and changes in efficacy

Inactive Publication Date: 2012-05-09
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, phenylethanol glycosides are considered to be the most important active ingredients of Cistanche. However, the chemical structure of phenylethanol glycosides contains caffeic acid ester bonds formed by caffeic acid and sugar. The ester bonds have poor stability and are prone to enzymatic and chemical hydrolysis. And the caffeoyl group of phenylethanol and the catechol group contained in the phenylethanol group are also easily oxidized, and the loss of phenylethanol is serious during processing and application.
[0007] The chemical structure of phenylethanol glycosides from Cistanche deserticola contains caffeic acid and sugar hydroxyl to form a caffeic acid ester bond. The ester bond has low stability and is prone to hydrolysis when the pH is greater than 5, resulting in the loss of active ingredients. The caffeoyl and phenylethyl alcohol groups contain o-diphenolic hydroxyl groups, which are prone to oxidation when the pH is greater than 5, resulting in a darker color of the product; the method of inactivating enzymes by heating can only inhibit enzymatic hydrolysis and browning, and cannot solve the problem of processing The decomposition and oxidation of phenylethanol glycosides from Cistanche deserticola caused by changes in chemical conditions during the process can only be partially solved by the currently disclosed technology. For example, only heat inactivation of enzymes is used for fresh Cistanche deserticola to avoid enzymatic decomposition of phenylethanol glycosides during decoction pieces or rough extraction. It does not involve the decomposition of phenylethanol glycosides caused by changes in the chemical environment during processing or application of preparations, resulting in changes in the efficacy of Cistanche products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a. 2 kilograms of fresh Cistanche salina are heated for 20 minutes with microwaves to inactivate the plant enzymes in the fresh Cistanche, and then broken into pieces after cooling, adding 30 grams of acetic acid by weight when breaking, and then adding 8 liters of 95% ethanol, Grind into fine pulp with a refiner, and the pH of the fine pulp is 3;

[0018] b. The fine slurry of step a is leached at a temperature of 80° C. for 2 hours, cooled, and filtered to separate the extract;

[0019] c, the step b extract is concentrated under reduced pressure through the film, and ethanol is recovered to obtain 1 kilogram of concentrated solution, and the concentrated solution is diluted to 2 liters with water, and the pH of the diluent is 3;

[0020] D, the adsorption resin column that 1 liter of HPD300 is housed on the dilution solution of step c is adsorbed, and the pH regulated by citric acid is 2 liters of water to wash, and the pH regulated by citric acid is the concentratio...

Embodiment 2

[0022] a. Heat 2 kg of fresh Cistanche deserticola with water at 100°C for 15 minutes to inactivate the plant enzymes in the fresh Cistanche deserticola, let it cool and then break it into pieces. When breaking, add 40 grams of phosphoric acid by weight, then add 6 liters of water, and pulverize it with a pulper. into a fine slurry, the pH of the fine slurry is 2;

[0023] b. The fine slurry of step a is leached at a temperature of 80° C. for 1 hour, cooled, and filtered to separate the extract;

[0024] c, the step b extract is concentrated to 2 liters with a blocking 200-300 industrial membrane;

[0025] d, 1 liter of HPD300 adsorption resin column is installed on the concentrated solution of step c for adsorption, and 3 liters of water whose pH is adjusted with malic acid is washed with 3 liters of water. After the liquid is concentrated under reduced pressure and spray-dried, 53.2 grams of total phenylethyl glycosides of Cistanche deserticola containing stabilizing agent ...

Embodiment 3

[0027]a. Heat 2 kg of fresh saline Cistanche for 10 minutes to inactivate the plant enzymes in the fresh Cistanche, let it cool and break it into pieces, add 20 grams of formic acid by weight when breaking, and then add ethanol with a concentration of 95% 8 liter, crushed into a fine slurry with a refiner, and the pH of the fine slurry is 2;

[0028] b. Extract the fine slurry in step a at a temperature of 80° C. for 2 hours, cool, and filter to separate the extract;

[0029] c. Concentrate the extract from step b by membrane filtration, reclaim ethanol to obtain 1 kg of concentrated solution, then dilute the concentrated solution to 2 liters with water, and the pH of the diluted solution is 3;

[0030] d, 1 liter of HPD300 adsorption resin column adsorption is housed on step c diluent, the pH that is regulated with caffeic acid is 2 liters of water washing, and the concentration that is 4 that the pH that is regulated with caffeic acid is 4 is eluted with 30% ethanol liquid a...

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PUM

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Abstract

The invention relates to a preparation method of cistanche deserticola phenylethanoid glycosides. The method comprises the steps of: cistanche deserticola crushing, lixiviating, absorbent resin adsorption, elution, and concentration, etc. In research of the extraction and stability of cistanche deserticola phenylethanoid glycoside, under a weakly acidic condition with pH of 2-4, the cistanche deserticola phenylethanoid glycoside can have substantially improved stability, and under the condition, the cistanche deserticola phenylethanoid glycoside extracted from cistanche deserticola is mainly composed of echinacoside and verbascoside. And in cistanche deserticola phenylethanoid glycosides, the total content proportion of echinacoside and verbascoside is greater than 90%. The content of cistanche deserticola phenylethanoid glycosides extracted by the method of the invention is obviously higher when compared with other extraction methods. And the obtained cistanche deserticola extract has stable effective component content and relative light color. The method provided in the invention avoids decomposition and oxidation of cistanche deserticola phenylethanoid glycoside, and is in favor of product stability.

Description

technical field [0001] The invention relates to a preparation method of phenethyl alcohol total glycosides of Cistanche deserticola, belonging to the technical field of medicine, food and health food processing and application. Background technique [0002] Cistanche deserticola is a traditional tonic Chinese medicine used to treat yin deficiency and impotence, female infertility, soreness and weakness of waist and knees, intestinal dryness and constipation. With the acceleration of the aging process of the world's population, aging-related diseases such as Alzheimer's disease seriously affect the quality of life of the elderly. As a traditional Chinese medicine for nourishing the kidney and nourishing the essence and blood, Cistanche deserticola has aroused in-depth understanding of Kampo medicine as early as the 1970s. The Japanese scholars of the study paid attention to and systematically studied the chemical components and pharmacological activities of Cistanche desertic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/64A61P39/06A61P1/14A61P15/00A61P15/10A61P15/08A61P9/00A61P25/28A61P7/02A61P17/00A23L1/29A23L33/00
Inventor 阿吉艾克拜尔・艾萨吴成赵丽君赵文军
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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