Method for abstracting active compound rich in echinacoside and feltwort glycoside

A technology of verbascoside and active substance, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, and can solve the problems of low dosage and short technological process.

Inactive Publication Date: 2008-06-11
广州绿色盈康生物工程有限公司 +1
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention proposes a production method for extracting active substances rich in echinacoside and verbascoside monomer compounds from fresh Cistanche deserticola fleshy stems with high yield through system integration technology, through cell crushing process, serous extraction process, membrane separation and The implementation of system integration technologies such as macroporous resin adsorption and chromatographic separation and purification process, high-temperature enzyme inactivation process, spray drying process, etc., the process flow is short, water is used as a solvent and the amount is very...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Select the fresh meaty stems of Cistanche cistanche as raw materials;

[0027] 2. Put the raw materials in a washing tank and rinse them with cold water at 10°C. After picking them up, put them in a common industrial crushing device and grinding device to crush and grind the fleshy stems of Cistanche deserticola to break up the cells; the process of crushing and grinding Charge into liquid nitrogen, and the concentration of liquid nitrogen in the Cistanche raw material is 0.5% (weight ratio); The measured material temperature is 12°C;

[0028] 3. Separating the milled Cistanche cell slurry with a common industrial centrifuge to obtain the Cistanche cell slurry;

[0029] 4. Selectively sieve and intercept macromolecular impurities such as polysaccharides and proteins, as well as impurities such as particles and sub-particles, through ultrafiltration membrane pores to selectively screen and intercept the Cistanche cell serum, and further selectively screen and intercep...

Embodiment 2

[0033] 1. Select the fresh meaty stems of Cistanche tubulosa that are kept fresh in an industrial refrigerator with a storage temperature of 4°C as raw materials;

[0034] 2. Rinse the fresh fleshy stems of Cistanche deserticola with industrially purified water, pick them up, and place them in a common industrial crushing device and milling device to crush and grind the fleshy stems of Cistanche deserticola so that the cells are broken; the crushing and grinding process is filled with CO 2 , CO in Cistanche 2 Concentration is 0.8% (weight ratio), and adds the ascorbic acid of 0.002% of raw material weight, the gluconolactone of 0.002% and the citric acid (weight ratio) of 0.003%; Recording material temperature is 8 ℃

[0035] 3. Separate the milled Cistanche cell slurry with an industrial centrifuge to obtain the Cistanche cell slurry. At the same time, add 200% pre-cooled industrial purified water (weight ratio) to the filter residue through two repetitions, and the water te...

Embodiment 3

[0040] 1. The quick-frozen Cistanche succulent stems refrigerated at -52°C in industrial freezers are used as raw materials;

[0041]2. Put the raw materials in a washing tank and rinse them with industrially pure water. After picking them up, place them in a common industrial crushing device and grinding device to crush and grind the fleshy stems of Cistanche deserticola to break up the cells; the crushing and grinding process The sodium sulfite (weight ratio) of 0.002% of raw material weight is added in the process; The recorded material temperature is 3°C;

[0042] 3. The milled Cistanche cell slurry is filtered by a common industrial filter press to obtain the Cistanche cell slurry; the filter residue is added to 60% industrial purified water (weight ratio), and further ground into a slurry by a common industrial grinder , and then separated by an industrial centrifuge to obtain the washing solution of Cistanche broken cells;

[0043] 4. After combining the Cistanche dese...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a system integration technology through cell disruption, slurry extraction, membrane separation and macroporous resin adsorption and chromatography separation and purification, high temperature enzyme deactivation and spray drying and the like, which achieves a technical process that from the time fresh cistanche raw materials are fed until obtaining active matters such as monomeric compounds which are rich in echinacoside and verbascoside and the like in 30 to 80 minutes, and the invention effectively overcomes a technical defect that active components such as benzyl carbinol glycosides compounds are normally seriously degraded and lost, which are caused by an enzymatic reaction which is initiated by an imperfect dry treatment method of cistanche, and the invention obviously improves the medical value of cistanche. In the active maters of cistanche which are obtained ( calculating by dry matter), the productivity of echinacoside monomeric compound reaches to 9.8% to 19.6%, and the yield of verbascoside monomeric compound reaches to 2.0% to 7.5%. The content of echinacoside monomeric compound in active matters of cistanche which are obtained reaches to 15% to 49.6%, and the content of verbascoside monomeric compound reaches to 5% to 26.7%.

Description

technical field [0001] The invention relates to a method for extracting active substances rich in echinacoside and verbascoside monomer compounds and the like from Cistanche deserticola with high yield. Background technique [0002] Herba Cistanches (Herba Cistanches) is a perennial parasitic herb of the genus Cistanche (Cistanche Hoffmg.et Link) in the family Cistanche. Cistanche deserticola was first recorded in "Shen Nong's Materia Medica" and listed as a top grade. It has the functions of nourishing kidney yang, nourishing essence and blood, moistening intestines and laxative; it is used to treat impotence in men, infertility in women, soreness of waist and knees, weakness of muscles and bones, dryness of the intestines and constipation. Since it was discovered that the phenylethanoid glycosides rich in Cistanche deserticola have significant curative effects on the treatment of vascular dementia, especially the potential effects of invigorating the kidney and yang and en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/64A61P25/00A61P9/00
Inventor 刘昕季青招淑燕张古忍李少松钟欣
Owner 广州绿色盈康生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap