Extraction and separation method for echinacoside and verbascoside in cistanche

A technology of verbascoside and cistanche extract, which is applied in the field of extracting active ingredients of traditional Chinese medicine, and can solve the problems of inapplicability to large-scale production, high production cost, and complicated preparation process

Inactive Publication Date: 2015-12-02
PUREDIA LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the separation of phenylethanol glycosides from Cistanche deserticola mainly focuses on the separation and preparation of echinacoside and verbascoside. Some processes only provide echinacoside separation methods or verbascoside separation methods, and

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  • Extraction and separation method for echinacoside and verbascoside in cistanche

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Add 50 kg of dried Cistanche dices and coarse powder, add 400 kg of 75% ethanol and use a multifunctional ultrasonic extraction tank to ultrasonically extract at 40°C for 1.5 hours, then extract once more according to the above method, combine the two extracts, and depressurize below 50°C Concentrate until there is no ethanol, filter and clarify the solution with ultrafiltration equipment to obtain a clarified solution of Cistanche deserticola extract; then adsorb the solution on a macroporous resin, wash with purified water until the effluent is clear, then elute with 55% ethanol, collect All the 55% ethanol eluate; concentrated with an external circulation vacuum concentrator, spray-dried the concentrated solution to obtain 9 kg of Cistanche cistanche phenylethanol glycoside extract, the content of echinacoside was 26% after testing, and the content of verbascoside was 11% .

Embodiment 2

[0063] Add 50 kg of dried Cistanche dices and coarse powder, add 400 kg of 75% ethanol and use a multifunctional ultrasonic extraction tank to ultrasonically extract at 40°C for 1.5 hours, then extract once more according to the above method, combine the two extracts, and depressurize below 50°C Concentrate until there is no ethanol, filter and clarify the solution with ultrafiltration equipment to obtain a clarified solution of Cistanche deserticola extract; then adsorb the solution on a macroporous resin, wash with purified water until the effluent is clear, then elute with 55% ethanol, collect All the 55% ethanol eluate; concentrated with an external circulation vacuum concentrator, spray-dried the concentrated solution to obtain 9.67 kg of Cistanche cistanche phenylethanol glycoside extract, the content of echinacoside was 26.71% after testing, and the content of verbascoside was 12% .

Embodiment 3

[0065] Add 50 kg of dried Cistanche dices and coarse powder, add 400 kg of 75% ethanol and use a multifunctional ultrasonic extraction tank to ultrasonically extract at 40°C for 1.5 hours, then extract once more according to the above method, combine the two extracts, and depressurize below 50°C Concentrate until there is no ethanol, filter and clarify the solution with ultrafiltration equipment to obtain a clarified solution of Cistanche deserticola extract; then adsorb the solution on a macroporous resin, wash with purified water until the effluent is clear, then elute with 55% ethanol, collect All the 55% ethanol eluate; concentrated with an external circulation vacuum concentrator, spray-dried the concentrated solution to obtain 9.5 kg of Cistanche cistanche phenylethanol glycoside extract, the content of echinacoside was 25% after testing, and the content of verbascoside was 10.6% .

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Abstract

The present invention relates to an extraction and separation method for echinacoside and verbascoside in cistanche. The method comprises the following steps: dicing the cistanche and drying the cistanche; adding 75% ethanol into a multi-functional ultrasonic extraction tank to extract for three times; combining extraction liquid and concentrating the extraction liquid until no ethanol exists; filtering and clarifying the extraction liquid and the concentration liquid of the cistanche by using ultrafiltration equipment; performing absorption on the liquid by using a macroporous resin column; firstly washing the liquid with water until the liquid is colorless, then eluting the liquid by using ethanol with certain concentration; collecting an elution solution that contains the echinacoside and the verbascoside; concentrating the elution solution by using an outer circulating vaccum concentrator; carrying out spray-drying on the concentration liquid to obtain the cistanche extract; and carrying out efficient liquid-phase tracing detection on the cistanche extract, wherein the echinacoside accounts for greater than or equal to 25%, and the verbascoside accounts for greater than or equal to 10%. The total content of phenylethanoid glycosides is greater than or equal to 80%, and the yield is 20%.

Description

1. Technical field [0001] The invention relates to a method for extracting active ingredients of traditional Chinese medicine, in particular to a method for extracting active ingredients containing echinacoside, verbascoside and the like from Cistanche cistanche. 2. Technical background [0002] Cistanche is mainly distributed in Xinjiang, Gansu, Ningxia, Inner Mongolia and other places, and has been artificially planted. It is a perennial parasitic herbaceous plant belonging to the genus Cistanche that parasitizes on the roots of Haloxylon Haloxylon and Tamarix, and is an endangered species of Liedangaceae. It has the functions of invigorating the kidney and strengthening yang, benefiting essence, delaying aging, and moisturizing the intestines. It is known as "desert ginseng". It has high medicinal value and is a traditional Chinese medicinal material in my country. [0003] Cistanche contains a variety of active ingredients, mainly including: phenylethanol glycosides, i...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
Inventor 袁木荣王启林昝超马二卜都朱永丙袁海佳
Owner PUREDIA LTD
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