Method for extracting oleuropein from olive leaves

A technology of olive leaves and oleuropein, which is applied in the field of extracting oleuropein, and can solve the problem of insufficient oleuropein products

Active Publication Date: 2011-11-02
陕西方舟制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the content of oleuropein in olive leaf extracts in the market is mostly 10-40%, but there are not many products with high content of oleuropein

Method used

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  • Method for extracting oleuropein from olive leaves
  • Method for extracting oleuropein from olive leaves
  • Method for extracting oleuropein from olive leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Crush 100g of cleaned olive leaves, add 1000ml of water, heat and extract at 60°C for 1 hour, filter, and collect the filtrate; add 1000ml of water to the olive leaves and heat and extract again, extract three times under the same conditions, combine the filtrate and concentrate to 600ml ;

[0027] After cooling to room temperature, the concentrated extract was loaded on the macroporous adsorption resin DM130, eluted with 50% methanol (volume ratio), and the eluate was collected;

[0028] Concentrate the collected eluent (liquid A) under reduced pressure to remove methanol, concentrate to no alcohol, let it cool down to room temperature, apply the concentrated solution to the decolorization resin XDA-7, and collect all the effluent (liquid B);

[0029] Extract with n-butanol of 1 times the volume of the effluent (liquid B), collect the organic phase, concentrate and dry to remove the organic solvent, and obtain 1.52 g of the extract.

[0030] Use HPLC method to detect ...

Embodiment 2

[0032] After crushing 100g of dried olive leaves, add 2000ml of 80% ethanol, heat and reflux for extraction for 1h, filter, and collect the filtrate; extract three times in total, combine the filtrate to concentrate to no alcohol, add water to 800ml, let cool to room temperature, and load the sample on the macropore Adsorbed resin DM130, eluted with 70% ethanol, and collected the eluate (solution A);

[0033] Concentrate the collected eluent (liquid A) under reduced pressure to remove ethanol, concentrate to no alcohol, let it cool down to room temperature, apply the concentrated solution to decolorizing resin LX-68, and collect the effluent (liquid B);

[0034] Extract with ethyl acetate of 0.8 times the volume of the effluent (liquid B), collect the organic phase (ethyl acetate phase), concentrate and dry to remove the organic solvent, obtain 1.21 g of the extract, and detect the content of oleuropein in the extract was 75.46%.

Embodiment 3

[0036] After crushing 100g of cleaned olive leaves, add 5000ml of 20% methanol, heat and reflux for extraction for 1h, filter, and collect the filtrate; extract three times in total, combine the filtrate and concentrate to 1000ml, let it cool to room temperature, and load it on macroporous adsorption resin D101 , eluted with 20% methanol, and collected the eluate (solution A);

[0037] Concentrate the collected eluate (liquid A) under reduced pressure to remove methanol, concentrate to no alcohol, let it cool down to room temperature, apply the concentrated solution to the decolorization resin LX-10G, and collect the effluent (liquid B);

[0038] Extract with ethyl acetate of 1.0 times the volume of the effluent (liquid B), collect the organic phase (ethyl acetate phase), concentrate and dry to remove the organic solvent, obtain 0.54 g of the extract, and detect the content of oleuropein in the extract is 93.22%.

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Abstract

The invention discloses a method for extracting oleuropein from olive leaves, which comprises the following steps of: extracting with water or a mixed solvent of alcohol and water, and then concentrating an extracting solution to separate the extracting solution through three steps in sequence: absorbing through using macroporous resins and eluting with a solvent of the alcohol and the water, absorbing through using decolouration resins, and extracting through using an organic solvent, wherein the macroporous resins have an excellent adsorption effect for the oleuropein and are used for eluting to remove other impurities, and the decolouration resins have an excellent adsorption effect for pigments in the olive extracting solution and are used for absorbing and removing pigments; separating and purifying the oleuropein in the extracting solution step by step; at last, acquiring an oleuropein extract with high content. The content of the oleuropein is more than 40 %, even up to 80-90 %.

Description

technical field [0001] The invention belongs to the technical field of oleuropein extraction, and relates to a method for extracting oleuropein from olive leaves. Background technique [0002] Olive leaf has been used medicinally for thousands of years, with the ancient Greeks using it to cleanse wounds. There are indications that drinking tea from olive leaves has been traditionally used in the Middle East for centuries to treat ailments such as coughs, sore throats, cystitis and fever. Additionally, olive leaf ointment is used to treat boils, rashes, warts and other skin ailments. Antioxidant active ingredients have been extracted from olive oil for a long time. Biologists have found that the antioxidant active ingredients contained in olive leaves are more prominent than olive oil, especially the content of oleuropein in leaves is significantly higher than that in fruits and trees. Scientists have found that the incidence of coronary heart disease and cancer in the Med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61K8/97A61P11/00A61P31/04A61P31/12A61P33/12A61P33/14A61Q19/00A61K127/00A61K8/9789
Inventor 雷玉萍侯睿刘涛张爱萍
Owner 陕西方舟制药有限公司
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