Extraction process for hydroxytyrosol from olive leaf

A technology of olive leaf hydroxyphenol and extraction process, which is applied in the preparation of organic compounds, sugar derivatives, sugar derivatives, etc., can solve the problems of high cost of raw materials and complicated preparation technology, meet market demand, and simplify the extraction process , The effect of low raw material cost

Inactive Publication Date: 2017-04-19
广西来宾绿翔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention discloses an extraction process of olive leaf hydroxytyrosol, which uses a percolation method to extract oleuropein products from olive leaves, and extracts hydroxytyrosol by hydrolyzing oleuropein to solve the complicated preparation technology in the existing extraction process. , high cost of raw materials and difficulty in realizing industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1: Crush olive leaves into coarse powder, pass through a 60-mesh sieve, add 80% ethanol at a weight ratio of 1:1 to seal and swell for 2 hours, put the fully expanded olive leaf coarse powder into a percolation barrel, and mix evenly Compact, add 80% ethanol solvent to carry out percolation extraction. When performing percolation extraction, the solvent should be continuously added to keep the olive leaf powder immersed in the solvent, and the coarse powder should be soaked for 24 hours before the percolation liquid flows out. When the percolate flows out, the outflow rate should be controlled at 2mL / min; when 75% of the percolate to be prepared is collected, stop the percolation, squeeze the dregs and combine the squeezed liquid with the percolate to obtain the obtained the required extract;

[0026] S2: The extract obtained in step S1 is adsorbed by D101 macroporous resin, and eluted with 60% ethanol at a flow rate of 3 BV / h for 30 minutes. After the elution is comp...

Embodiment 2

[0030] S1: Crush olive leaves into coarse powder, pass through a 60-mesh sieve, add 80% ethanol at a weight ratio of 1:1 to seal and swell for 2 hours, put the fully expanded olive leaf coarse powder into a percolation barrel, and mix evenly Compact, add 80% ethanol solvent to carry out percolation extraction. When performing percolation extraction, the solvent should be continuously added to keep the olive leaf powder immersed in the solvent, and the coarse powder should be soaked for 24 hours before the percolation liquid flows out. When the percolate flows out, the outflow rate should be controlled at 2mL / min; when 75% of the percolate to be prepared is collected, stop the percolation, squeeze the dregs and combine the squeezed liquid with the percolate to obtain the obtained the required extract;

[0031] S2: The extract obtained in step S1 is adsorbed by D101 macroporous resin, and eluted with 60% ethanol at a flow rate of 3 BV / h for 20 minutes. After the elution is comp...

Embodiment 3

[0035] S1: Crush olive leaves into coarse powder, pass through a 60-mesh sieve, add 80% ethanol at a weight ratio of 1:1 to seal and swell for 2 hours, put the fully expanded olive leaf coarse powder into a percolation barrel, and mix evenly Compact, add 80% ethanol solvent to carry out percolation extraction. When performing percolation extraction, the solvent should be continuously added to keep the olive leaf powder immersed in the solvent, and the coarse powder should be soaked for 24 hours before the percolation liquid flows out. When the percolate flows out, the outflow rate should be controlled at 3mL / min; when 75% of the percolate is collected, stop the percolation, squeeze the dregs and combine the squeeze liquid with the percolate to obtain the the required extract;

[0036] S2: The extract obtained in step S1 is adsorbed by D101 macroporous resin, and eluted with 60% ethanol at a flow rate of 3 BV / h for 25 minutes. After the elution is complete, the eluate is conce...

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Abstract

The invention discloses an extraction process for hydroxytyrosol from an olive leaf, belonging to the technical field of extraction of active components from medicinal plants. The extraction process comprises the following steps: crushing the olive leaf into coarse powder, carrying out percolation extraction by using ethanol with a concentration of 80%, subjecting liquid extract to adsorption with D101 resin, then carrying out elution by using ethanol with a concentration of 60% and carrying out concentration and drying on eluate so as to obtain oleuropein with a concentration of 45 to 55%; and hydrolyzing oleuropein with a sodium hydroxide solution, adding acid for neutralization after complete hydrolysis, carrying out precipitation and centrifugation, subjecting obtained centrifugate to adsorption with D101 resin and elution with hot ethanol, concentrating eluate, then repeatedly carrying out extraction with ethyl acetate four times, and carrying out concentration and drying on ethyl acetate-layer liquid so as to obtain hydroxytyrosol with a concentration of 80 to 90%. The extraction process for hydroxytyrosol from the olive leaf is high in the utilization rate of raw materials, and the prepared oleuropein and hydroxytyrosol can greatly meet market demands.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of extracting active ingredients of medicinal plants, and in particular relates to an extraction process of olive leaf hydroxytyrosol. [0003] 【Background technique】 [0004] Olive is a famous subtropical specialty fruit tree. It belongs to arbor plants. Its fruit can be eaten raw or soaked. It can be used medicinally to cure laryngitis, hemoptysis, polydipsia, enteritis and diarrhea. It also has dual functions of medicine and food. Effectiveness of phenolic substances in olive leaves—Hydroxytyrosol has various biological activities such as antioxidant, anti-platelet aggregation, anti-atherosclerosis, anti-cancer and antibacterial, and has been widely used in food, health products, cosmetics and In industries such as medical treatment, its demand is increasing year by year. In recent years, there are many methods for the separation and purification of hydroxytyrosol from olive leaves, but most of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/00C07C37/70C07C39/11C07H17/04C07H1/08
CPCC07C37/00C07C37/70C07H1/08C07H17/04C07C39/11
Inventor 陆兴国韦小兰杨红英
Owner 广西来宾绿翔生物科技有限公司
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