Process method for extracting total cardiac glycoside from Calotropis gigantea
A process method and technology of croissant melon are applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., which can solve the problem of low purity of total cardiac glycosides in croissant melon, improve yield and purity, and achieve separation and extraction. Simple process, simple operation effect
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Embodiment 1
[0028] S1: Crush the stems and leaves of croissant melon into coarse powder, pass through a 30-mesh sieve, add 60% ethanol aqueous solution at a weight ratio of 1:1, seal and swell for 1 hour, and put the fully swelled croissant melon coarse powder into reflux In the extractor, add 5 times the amount of 60% ethanol aqueous solution as a solvent to carry out reflux extraction for 1 hour, concentrate the extract, settle, put it into a centrifuge at 1000rpm for centrifugation, and dry the obtained centrifugate to obtain total cardiac glycosides of crocus melon. Through ultraviolet-visible spectrophotometry detection, the mass content of the total cardiac glycosides in the product is 3%, and the recovery rate of the total cardiac glycosides in the product is calculated to be 57.53%.
[0029] S2: After concentrating the extract obtained in step S1, it is absorbed by D101 macroporous resin, and then eluted with 40% ethanol aqueous solution at a flow rate of 2 BV / h for 30 minutes. Aft...
Embodiment 2
[0033] S1: Crush the stems and leaves of croissant melon into coarse powder, pass through a 40-mesh sieve, add 70% ethanol aqueous solution at a weight ratio of 1:1, seal and swell for 1 hour, and put the fully swelled croissant melon coarse powder into reflux In the extractor, add 5 times the amount of 70% ethanol aqueous solution as a solvent to carry out reflux extraction for 1.5h, the number of extractions is twice, the combined extracts are concentrated, settled, put into a centrifuge at 1000rpm for centrifugation, and the obtained centrifuge is dried to produce To obtain the total cardiac glycosides of croissanthus. Detection by ultraviolet-visible spectrophotometry shows that the content of total cardiac glycosides in the product is 4%, and the recovery rate of total cardiac glycosides in the product is calculated to be 76.71%.
[0034] S2: After concentrating the extract obtained in step S1, it is absorbed by D101 macroporous resin, and then eluted with 50% ethanol aqu...
Embodiment 3
[0038] S1: Crush the stems and leaves of croissant melon into coarse powder, pass through a 30-mesh sieve, add 80% ethanol aqueous solution at a weight ratio of 1:1, seal and swell for 1 hour, and put the fully expanded croissant melon coarse powder into reflux In the extractor, add 5 times the amount of 80% ethanol aqueous solution as a solvent and carry out reflux extraction for 2 hours. The number of extractions is three times. After combining the extracts, they are concentrated, settled, put into a centrifuge at 1000rpm for centrifugation, and the obtained centrifuge is dried to obtain ox horns. Total cardiac glycosides of melons. Detection by ultraviolet-visible spectrophotometry shows that the content of total cardiac glycosides in the product is 5%, and the recovery rate of total cardiac glycosides in the product is calculated to be 95.89%.
[0039]S2: After concentrating the extract obtained in step S1, it is absorbed by D101 macroporous resin, and then eluted with 60%...
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