Preparation method of olive leaf extract

An olive leaf extract and olive leaf technology, applied in the field of preparation of olive leaf extract, can solve the problems of high cost and difficulty in realizing large-scale production, and achieve the effects of low cost, reduced energy consumption and high yield

Active Publication Date: 2015-11-18
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although a higher oleuropein content is obtained in this method, the cost is relatively high, it is more suitable for scientific research, and it is difficult to achieve large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: a kind of olive leaf extract, it is made by following method, comprises the steps:

[0039] (1) After soaking the olive leaves in salt water for 1 hour, dry them and grind them into 40-60 mesh fine powder;

[0040] (2) Add 5 times of water to the raw material, add cellulase:alkaline protease 1:3 compound enzyme, the amount of compound enzyme is 1% by weight of the raw material, keep enzymolysis at 30-50°C for 0.5h, filter, Obtain enzymatic solution;

[0041] (3) The enzymatic hydrolysis solution is directly passed through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:4~1:15, and it is eluted with 1-3 times the column volume of water and 1-2 times the column volume of 95% ethanol solution respectively. Remove impurities, elute with 0.2-0.5% glacial acetic acid-50% ethanol (1:1) solution of 6 times the column volume, and collect the eluate;

[0042] (4) After evaporating glacial acetic acid and ethanol in the eluent, m...

Embodiment 2

[0043] Embodiment 2: a kind of olive leaf extract, it is made by following method, comprises the steps:

[0044] (1) After soaking the olive leaves in salt water for 2 hours, dry them and grind them into fine powder;

[0045] (2) Add 3 times water to the raw material, add cellulase:alkaline protease compound enzyme, the amount of compound enzyme is 2% by weight of the raw material, keep enzymolysis at 30-50°C for 0.5h, filter to obtain enzymolysis liquid;

[0046] (3) The enzymatic hydrolysis solution passes directly through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:4 to 1:15, and is eluted with 1-3 column volumes of water and 1-2 column volumes of 95% ethanol solution respectively Remove impurities, elute with 0.2-0.5% glacial acetic acid-50% ethanol (1:1) solution of 10 times the column volume, and collect the eluate;

[0047] (4) After evaporating glacial acetic acid and ethanol in the eluent, microwave vacuum drying, temperatur...

Embodiment 3

[0048] Embodiment 3: a kind of olive leaf extract, it is made by following method, comprises the steps:

[0049] (1) After soaking the olive leaves in salt water for 1 hour, dry them and grind them into 50-60 mesh fine powder;

[0050] (2) Add 7 times of water to the raw material, add cellulase:alkaline protease 1:3 compound enzyme, the amount of compound enzyme is 1% by weight of the raw material, keep enzymolysis at 30-50°C for 0.5h, filter, Obtain enzymatic solution;

[0051] (3) The enzymatic hydrolysis solution passes directly through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:4 to 1:15, and is eluted with 1-3 column volumes of water and 1-2 column volumes of 95% ethanol solution respectively Remove impurities, elute with 0.2-0.5% glacial acetic acid-50% ethanol (1:1) solution of 8 times the column volume, and collect the eluate;

[0052] (4) After evaporating glacial acetic acid and ethanol in the eluent, microwave vacuum dryi...

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PUM

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Abstract

The invention discloses a preparation method of an olive leaf extract and the olive leaf extract prepared by the preparation method. An olive leaf extract product of which the oleuropein content is greater than 90% is obtained through the processes of infiltration, enzymatic extraction, separation and drying on olive leaves. The whole method only comprises four steps, the adopted reagents are common non-toxic ingredients, the yield is high, the cost is low, the obtained product can be directly used for preparing medicines, health products and foods, and the method is very suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of olive leaf extract, which belongs to the field of natural plant extracts. Background technique [0002] Olive (Oleaeuropaea L.), an evergreen tree belonging to the genus Olea in the family Oleaceae, is a world-renowned woody oil tree species with a cultivation history of more than 4,000 years. Olives are mainly distributed between 45° north latitude and 37° south latitude. The central distribution area is the Mediterranean countries. Greece, Italy, Tunisia, and Spain are the concentrated production areas. Now all countries in the world including China have introduced and cultivated them. Since the introduction of olives in my country in 1964, in the past ten years, with the support of the western development policy, the cultivation of olives has developed rapidly. The construction of bases in Gansu and Sichuan provinces has begun to take shape, and olive oil processing plants have been established. Olive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/63
Inventor 赵永强高瑞马亚琼张成亮
Owner 内蒙古昶辉生物科技股份有限公司
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