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Preparation method of sweet-scented osmanthus seed extract, sweet-scented osmanthus seed extract and application of sweet-scented osmanthus seed extract

A technology for extracts and seeds, applied in the field of Osmanthus fragrans seed extract and application thereof, and the preparation field of Osmanthus fragrans seed extract, can solve the problem of easy-to-destroy active ingredient espressoside, unfavorable espressoside purity and yield, and preparation instruments Expensive and other problems, to achieve the effect of inhibiting platelet aggregation, preventing and treating heart palsy, and simple process

Inactive Publication Date: 2019-08-02
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-purity privetin has been obtained in the above-mentioned related method for obtaining privetin from Ligustrum lucidum, there are more or less problems such as more operation steps, long preparation process, and expensive preparation equipment; at the same time, although The patent (CN106632544A) is to obtain the tercitrus glycoside monomer through two steps, but in the first step of the medium-pressure preparation step, a medium-pressure preparation chromatograph is required, and the price of this instrument is relatively expensive, and it is not easy to scale up the pilot production
In addition, the method involving the separation and preparation of privetin from osmanthus seeds also has the following problems: the extraction methods such as heating and reflux are easy to destroy the active ingredient privetin, which is not conducive to improving the purity and yield of privetin

Method used

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  • Preparation method of sweet-scented osmanthus seed extract, sweet-scented osmanthus seed extract and application of sweet-scented osmanthus seed extract
  • Preparation method of sweet-scented osmanthus seed extract, sweet-scented osmanthus seed extract and application of sweet-scented osmanthus seed extract
  • Preparation method of sweet-scented osmanthus seed extract, sweet-scented osmanthus seed extract and application of sweet-scented osmanthus seed extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of osmanthus fragrans seed extract, comprises the following steps:

[0031] S1. Take 5 kg of sweet-scented osmanthus seeds, peel and pulverize, add 50 L of ethanol solution with a volume concentration of 95%, and mix evenly to obtain a mixture.

[0032] S2. Perform percolation extraction on the mixture obtained in step S1, specifically, continuously add ethanol solution with a fresh volume concentration of 95% from above the percolator, and collect percolation liquid at the outlet below the percolator at the same time. 25L. In this step, the number of times of percolation extraction is 3 times, and the time of single percolation extraction is 12h.

[0033] S3. After each percolation extraction is completed, use a rotary evaporator at 40° C. to recover ethanol in the extract under reduced pressure, and combine the obtained concentrates to obtain an osmanthus seed extract.

Embodiment 2

[0035] Evaluation of the Anti-cardiac Activity of Osmanthus fragrans Seed Extract Monomer

[0036] With the sweet-scented osmanthus seed extract that makes in embodiment 1 as tested substance, investigate following experiment:

[0037] Effects on ADP-induced Platelet Aggregation in Rabbits

[0038]Take healthy Japanese big-eared white rabbits weighing 2.5-3.0 kg, and take 3 mL of blood from the ear vein (anticoagulant: blood = 1:9), mix immediately, seal, and centrifuge at 500 rpm for 10 minutes to prepare platelet-rich plasma (PRP). After the PRP was separated, it was centrifuged again (3000rpm / min, 10min) to prepare platelet-poor plasma (PPP). Take the PRP and put it into a 300 μL test cup with magnetic beads, mix the remaining blood, and centrifuge at 3000 rpm for 10 minutes. Take PPP and put it into a 300 μL comparison test cup. After zeroing with PRP, take 200 μL PRP and add 5 μL equimolar concentration of the test substance DMSO (dimethyl sulfoxide) solution, solvent ...

Embodiment 3

[0054] With the sweet-scented osmanthus seed extract that makes in embodiment 1 as tested substance, investigate its effect on rat in vitro cardiomyocyte hypoxic injury protection

[0055] Culture of neonatal rat cardiomyocytes

[0056] Wistar suckling mice aged 1-3d were soaked and sterilized with 75% (unit: v / v) ethanol, and the apical tissue was taken, cut into pieces, placed in a centrifuge tube, and washed with 10 mL, 0.25% (unit: v / v) pancreatic The protease solution was digested in a constant temperature shaker at 37°C in a water bath for 10 minutes, and at the same time, the tissue was pipetted for 2 minutes to allow natural precipitation. After the precipitation is complete, take the supernatant to another tube, add 2mL cold medium to stop the digestion, then centrifuge for 10min, discard the supernatant, add 8mL D-Hanks solution to the precipitation, and finally use 20% (unit It is the DMEM medium of v / v) fetal bovine serum, and the cardiomyocyte pellet is mixed to ...

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Abstract

The invention discloses a preparation method of a sweet-scented osmanthus seed extract, the sweet-scented osmanthus seed extract and application of the sweet-scented osmanthus seed extract. Accordingto the preparation method of the sweet-scented osmanthus seed extract, specnuezhenide in sweet-scented osmanthus seeds is extracted in a percolation extracting mode; the sweet-scented osmanthus seed extract is obtained. According to the preparation method of the sweet-scented osmanthus seed extract, the percolation extracting mode is used, so that the damage to the effective ingredient of the specnuezhenide in the sweet-scented osmanthus seeds can be avoided; when the extract is used for preparing heart impediment resistant medicine or health care products, the domestic rabbit platelet aggregation effect can be obviously inhibited, the metabolic rate of myocardial cells is improved, the anti-inflammatory activity is improved, and the thrombus formation is inhibited; very important significance is realized for preventing and treating heart impediment diseases and developing new medicine. Meanwhile, the preparation method has the advantages that the process is simple; the operation is convenient; the cost is low; the requirements on instruments are low; the mass production is easy, and the like. The secondary utilization value of the sweet-scented osmanthus seeds can be developed andutilized to the greatest degree; relatively high use values and application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to a preparation method of an osmanthus seed extract, an osmanthus seed extract and applications thereof. Background technique [0002] Xinbi, Tongxinguan two characters, that is, the meaning of blockage. Cardiorrhagia refers to the blockage of the heart arteries in the "Internal Classics", which is a disease with heartache as the main symptom caused by Qi and blood obstruction. Heart pain and heart pain are related to rheumatic heart disease and coronary heart disease angina pectoris described in modern medicine. Out of balance. The clinical manifestations are angina pectoris and myocardial infarction. [0003] Coronary heart disease angina pectoris is caused by an imbalance between myocardial oxygen demand and oxygen supply due to coronary artery sclerosis stenosis of more than 60% of the lumen, resulting in myocardial ischemia. Myocardial infarction is caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/63A61K31/7048A61P29/00A61P9/00A61K131/00
CPCA61K31/7048A61K36/63A61K2236/333A61P9/00A61P29/00
Inventor 唐伟卓赵晨曦韩晗陈建荣
Owner CHANGSHA UNIVERSITY
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