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Quality detection method of ligusticum wallichii shampoo extract

A quality detection method and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult separation of active ingredients, high difficulty in qualitative and quantitative detection, and low quality, so as to achieve product safety and effectiveness guarantee , good application prospects, good repeatability

Inactive Publication Date: 2019-10-01
SICHUAN GREEN HERB TECH DEV CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the Ligusticum chuanxiong shampoo extract developed by the research institute affiliated to Sichuan Green Materia Medica Technology Development Co., Ltd. is made from traditional Chinese medicines such as Ligustrum lucidum, Eclipta, Polygonum multiflorum, Galla gall, and Ligusticum chuanxiong, which are commonly used in the treatment of hair loss. The effect of hair loss and growth is also very helpful for patients with psoriasis. However, the effective material basis of traditional Chinese medicine compound is complex, the active ingredients are difficult to separate, and the qualitative and quantitative detection is difficult. Therefore, there is still a lack of quality detection methods, which leads to The quality of the products is uncontrollable and unstable, and it is difficult to distinguish the counterfeit and low-quality Chuanxiong shampoo produced from traditional Chinese medicines such as Ligustrum lucidum, Eclipta, Polygonum multiflorum, Galla gall, and Chuanxiong.

Method used

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  • Quality detection method of ligusticum wallichii shampoo extract
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  • Quality detection method of ligusticum wallichii shampoo extract

Examples

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Embodiment 1

[0047] The identification of Ligustrum lucidum in embodiment 1 Chuanxiong shampoo extract

[0048] 1) Preparation of reference substance solution: take oleanolic acid reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference substance solution;

[0049] 2) Preparation of the test solution: take 10 g of the shampoo extract powder, add 100 ml of chloroform, ultrasonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 0.5 ml of chloroform to the residue to dissolve it, and use it as the test solution;

[0050] 3) Draw up 4 and 10 μl of the reference substance solution and the test solution, respectively, and place them on the same silica gel G thin-layer plate, use chloroform-methanol-formic acid (40:1:1) as the developer, develop, take out, Dry it in the air, spray it with 10% sulfuric acid ethanol solution, heat it at 105°C until the spots are clear in color, take it out, and inspect it under an ultraviolet lamp (366nm). TLC ...

Embodiment 2

[0053] The identification of Eclipta chinensis in embodiment 2 Chuanxiong shampoo extract

[0054] ①Preparation of the reference substance solution: Take an appropriate amount of Eclipta glucoside A reference substance, add methanol to make a solution containing 0.5mg per 1ml, and use it as the reference substance solution;

[0055] ②Preparation of the reference medicinal material solution: take 1 g of Eclipta as reference medicinal material, add 10 ml of 70% methanol, sonicate for 45 minutes, filter, evaporate the filtrate to dryness, add 0.5 ml of methanol to the residue to dissolve, and use it as the reference medicinal material solution;

[0056] ③Preparation of the test solution: Take 4g of shampoo extract powder, add 40ml of 70% methanol, sonicate for 45min, filter, evaporate the filtrate to dryness, add 0.5ml of methanol to dissolve the residue, and use it as the test solution.

[0057] ④ Take 10 μl each of the test solution and the reference medicinal material solution...

Embodiment 3

[0059] The identification of Galla gall in the extract of Rhizoma Chuanxiong shampoo in embodiment 3

[0060] a, preparation of reference substance solution: take gallic acid reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference substance solution;

[0061] b. Preparation of the reference medicinal material solution: take 5 g of the Galla gall control medicinal material, add 50 ml of 4mol / L hydrochloric acid solution, sonicate for 60 min, filter, extract twice with an equal volume of ethyl acetate, combine the ethyl acetate extracts, evaporate to dryness, Dissolve with 5ml of methanol as the reference medicinal solution;

[0062] c. Preparation of test solution: Take 5g of shampoo extract powder, add 50ml of 4mol / L hydrochloric acid solution, ultrasonically treat for 60min, filter, extract twice with equal volume of ethyl acetate, combine the ethyl acetate extract, evaporate After drying, dissolve with 5ml of methanol as the test soluti...

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Abstract

The invention provides a quality control method of a ligusticum wallichii shampoo extract. The method adopts thin layer chromatography to identify glossy privet fruit, eclipta and gallnut therein, andmeasures the specnuezhenide by high performance liquid chromatography. The quality detection method of the ligusticum wallichii shampoo extract can be used for identifying glossy privet fruit, eclipta and gallnut and measuring the content of specnuezhenide, and can also be used for qualitatively identifying the ligusticum wallichii shampoo water extract and measuring the content of main components. The method is simple and convenient, good in repeatability and reliable in result, provides a method for quality control of the ligusticum wallichii shampoo, guarantees safety and effectiveness ofproducts, and has good application prospects.

Description

technical field [0001] The invention specifically relates to a quality detection method for the extract of Rhizoma Chuanxiong shampoo. Background technique [0002] With the improvement of people's living standards, the pace of life has accelerated, and the pressure of work and study has also increased. In a state of mental stress, more and more people will experience severe hair loss and hair loss, resulting in an unsightly image and impaired confidence. The hair that falls out normally is the hair in the catagen and telogen phase. Since the hair entering the catagen and the newly entering the growth phase are constantly in a dynamic balance, it can maintain a normal amount of hair. Pathological alopecia is the abnormal or excessive loss of hair, which has many causes. Traditional Chinese medicine believes that the main causes of hair loss are insufficient essence and blood, stagnation of blood stagnation, accumulation of phlegm dampness, blood heat and wind dryness, and ...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
CPCG01N30/02G01N30/90
Inventor 周厚成胡昌江周巧敏段鑫吴永超余钡杨煜
Owner SICHUAN GREEN HERB TECH DEV CO LTD
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