Preparation method of specnuezhenide, and specnuezhenide and application of preparation method

Abstract

The invention discloses a preparation method of specnuezhenide, and specnuezhenide and an application of the preparation method. The preparation method comprises the following steps of extracting thespecnuezhenide from osmanthus fragrans seeds by a percolating extraction method, so as to obtain an osmanthus fragrans seed extract; sequentially leaching specnuezhenide in the osmanthus fragrans seedextract through petroleum ether, dichloromethane and n-butanol so as to obtain an n-butanol layer leaching substance; and performing two-step normal-phase column chromatography on the n-butanol layerleaching substance so as to obtain specnuezhenide. According to the preparation method disclosed by the invention, the osmanthus fragrans seeds are used as raw materials, through operations of percolating extraction, fractional leaching, two-step normal-phase column chromatography and the like, the specnuezhenide of which the purity is as high as 95% or above is prepared. When the specnuezhenideis used for preparing cardiac obstruction resisting medicines or health-care products, the aggregation effect of platelet of rabbits can be significantly restrained, the metabolic rate of cardiac muscle cells can be increased, antiinflammatory activity is improved, the formulation of thrombosis is restrained, and the specnuezhenide has important significance in preventing and treating cardiac obstruction diseases and developing new medicines.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a preparation method of privetin, privetin and applications thereof. Background technique [0002] Xinbi, Tongxinguan two characters, that is, the meaning of blockage. Cardiorrhagia refers to the blockage of the heart arteries in the "Internal Classics", which is a disease with heartache as the main symptom caused by Qi and blood obstruction. Heart pain and heart pain are related to rheumatic heart disease and coronary heart disease angina pectoris described in modern medicine. Out of balance. The clinical manifestations are angina pectoris and myocardial infarction. [0003] Coronary heart disease angina pectoris is caused by an imbalance between myocardial oxygen demand and oxygen supply due to coronary artery sclerosis stenosis of more than 60% of the lumen, resulting in myocardial ischemia. Myocardial infarction is caused by severe stenosis or even com...

AI Technical Summary

Problems solved by technology

Although high-purity privetin has been obtained in the above-mentioned related method for obtaining privetin from Ligustrum lucidum, there are more or less problems such as more operation steps, long preparation process, and expensive preparation equipment; at the same time, although The patent (CN106632544A) is to obtain the tercitrus glycoside monomer through two steps, but in the first step of the medium-pressure preparation step, a medium-pressure preparation chromatograph is required, and the price of this instrument is relatively expensive, and it is not easy to scale up the pilot production

In addition, the method involving the separation and preparation of privetin from osmanthus seeds also has the following problems: the extraction methods such as heating and reflux are easy to destroy the active ingredient privetin, which is not conducive to improving the purity and yield of privetin; In the extraction method, the extractant contains ethyl acetate, and the use of ethyl acetate is not conducive to the removal of low-polarity impurities, which is not conducive to improving the extraction quality of n-butanol extracts; the separation technology used includes MCI column, normal phase silica gel column and reversed-phase silica gel column in three steps, the operation is relatively complicated, and it is not easy to be used industrially. At the same time, the eluent in the separation technology used contains dichloromethane, and dichloromethane has problems such as high toxicity and high cost.

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