Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing specnuezhenide

A technology of privet privet and privet privet, which is applied in the field of high-purity privet privet, and can solve problems such as complex processes and operations, and unsuitability for large-scale preparation of pure privet privet

Inactive Publication Date: 2012-03-14
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can separate and purify high-purity privetin, but the process and operation are relatively complicated, and it is not suitable for mass production of high-purity privetin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing specnuezhenide
  • Method for preparing specnuezhenide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 10kg of Ligustrum lucidum medicinal material, pulverize it, add 10 times, 8 times, and 8 times the amount of medicinal material to decoct 3 times, decoct for 2 hours each time, filter each time and combine the filtrate, and the filtrate is properly concentrated; the concentrated solution is sampled in On the D-101 macroporous resin column, first elute with water, discard the washing liquid, then elute with 20% ethanol, collect the eluate, concentrate to a liquid extract; add methanol to stir fully, place the precipitate, filter, The filtrate was evaporated to dryness; finally separated and purified with a normal phase silica gel column and a reverse silica gel column to obtain about 28g of a compound with a purity of 98%, combined with the NMR identification results and existing literature research spectrum data, it was determined that the compound was Privet privet Glycosides.

Embodiment 2

[0023] Take Ligustrum lucidum medicinal material, crush it; weigh 203g, add 400ml of water to soak for 30min, then add 1200ml of water to extract for 2h, filter, then add 1600ml of water to extract twice, filter, combine the three filtrates, and concentrate the filtrate to about 400ml; load the sample on D- 101 macroporous resin column, first eluted with water, discarded the water eluent, then eluted with 40% ethanol, and the eluate was concentrated to a liquid extract (about 40ml); add industrial alcohol (95% ethanol) 200ml, Fully stirred and shaken, left to precipitate for 2 hours, filtered, and the filtrate was evaporated to dryness; then purified with a normal phase silica gel chromatography column to obtain 3.375 g of the crude product of teruci, with a purity of 81.18%, and the yield at this time was 63.61%. Purified by reverse silica gel column chromatography, 1.440 g of pure privetin was obtained, with a purity of 98.13%, and a yield of 21.55%.

Embodiment 3

[0025] According to the method for preparing privetin according to the patent CN101704857A, take 227g of the pulverized privet privet medicinal material, purify it to obtain 1.853g of pure privet, and its content is 98.40% as measured by HPLC. At this time, the yield of privet was 20.03%. By comparison, it is found that the yield of the invention patent CN101704857A for preparing terucitoside is slightly lower than that of the patented method. Example 2 produced 1.440g of pure privetin, with a purity of 98.13%, and its cost was calculated at 799.3 yuan / g with reference to the prices of various reagents and materials purchased in the laboratory; the patent CN101704857A preparation method produced pure privetin Product 1.853g, content is 98.40%, and the calculation cost with the cost calculation method of embodiment two is 925.0 yuan / g. When the dosage is increased to 100kg, the cost is further reduced, combined with further reasonable calculation and calculation of the market ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for extracting specnuezhenide crude products (higher than 80 percent) and high-purity specnuezhenide (higher than 98 percent) from fructus ligustri lucidi, which belongs to the field of natural medicine chemistry. The fructus ligustri lucidi is used as a raw material, medicine materials are firstly crushed, a water decoction extraction method is selected, extracting solution is rarefied by macroporous resin columns after being slightly concentrated, then, the alcohol deposition treatment is carried out, and finally, the positive silica gel column purification and the opposite silica gel column purification are selected for preparing the specnuezhenide with different content requirements. The method can be used for preparing the specnuezhenide with lower content in a scale way, the yield is high, the operation is simple, and the cost is low; and the specnuezhenide with higher content can also be prepared, the yield is lower, the cost is higher, the selection can be carried out according to the content requirement, raw material parts and reagent parts during the preparation by the two method can be repeatedly utilized, and the preparation cost is lower than that of the existing preparation method.

Description

technical field [0001] The invention belongs to the field of natural medicine chemistry, and relates to a method for extracting crude privetin (>80%) and high-purity privetin (>98%) from Ligustrum lucidum. Background technique [0002] Ligustrum lucidum Ait. is the dry ripe fruit of Ligustrum lucidum Ait. Ligustrum lucidum mainly contains chemical components such as triterpenoids, iridoids, phenethyl alcohols, and flavonoids. Specnuezhenide, the English name is Specnuezhenide, a split ring iridoid glycoside in Ligustrum lucidum, as a component with strong specificity, it was first recorded as the medicinal material of Ligustrum lucidum in the 2010 edition of Chinese Pharmacopoeia The index component of content determination, its molecular formula is C 31 h 42 o 17 , with a molecular weight of 686.5, which is a relatively high content component in Ligustrum lucidum, and its pharmacological research is less, and studies have found that it has pharmacological effects ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
CPCY02P20/582
Inventor 王飞章从恩陈前锋张国林
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com