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Establishment method for near-infrared quantitative correction model of Zhenqi Fuzheng Preparations and detection method for Zhenqi Fuzheng Preparations

A technology for straightening and calibrating models of Zhenqi is applied in the field of infrared detection, which can solve the problems of time-consuming and labor-intensive detection, complex sample processing, and high cost, and achieve the effects of reducing detection costs, efficient detection methods, and high accuracy.

Inactive Publication Date: 2019-02-15
JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT
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  • Application Information

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Problems solved by technology

However, the traditional HPLC method is time-consuming, labor-intensive, complex sample processing, and high cost.

Method used

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  • Establishment method for near-infrared quantitative correction model of Zhenqi Fuzheng Preparations and detection method for Zhenqi Fuzheng Preparations
  • Establishment method for near-infrared quantitative correction model of Zhenqi Fuzheng Preparations and detection method for Zhenqi Fuzheng Preparations
  • Establishment method for near-infrared quantitative correction model of Zhenqi Fuzheng Preparations and detection method for Zhenqi Fuzheng Preparations

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preparation example Construction

[0059] (3) Preparation of the reference substance solution: each take an appropriate amount of the reference substance of privetin, salidroside, and calycosin glucoside, accurately weighed, and add methanol to make concentrations of 0.2mg / ml, 0.1mg / ml, 0.05mg / ml mixed reference substance solution, filtered, to obtain; take an appropriate amount of astragaloside IV reference substance, accurately weighed, add methanol to make a reference substance solution with a concentration of 0.1mg / ml, filter, to obtain.

[0060] Preparation of the test solution: take the finished product powder (through No. 3 sieve), accurately weigh (1.0-3.0 g for the sugar-containing type; 0.3-1.0 g for the sugar-free type), put it in a stoppered conical flask, and accurately add 50- 10-50mL of 100% methanol, weighed, ultrasonically treated (200W, 40kHz) for 20-60 minutes, let cool, weighed again, made up the lost weight with 50-100% methanol, shake well, filter, take Continue the filtrate, that is.

[...

Embodiment 1

[0066] Embodiment 1 near-infrared detection index

[0067] 1.1 Near-infrared modeling indicators of salidroside, teruzhenin, calycosin glucoside, and astragaloside IV

[0068] 1.1.1 Modeling method

[0069] Collect 56 batches of finished products of Zhenqi Fuzheng Granules, and use HPLC to determine the content of salidroside, teruci, calycosin glucoside, and astragaloside IV. Multivariate regression method was used to establish the correlation model between the NIR spectrum of Zhenqi Fuzheng Granules and the measured values ​​of salidroside, prigulin, calycosin glucoside, and astragaloside IV in liquid phase spectra.

[0070] 1.1.2 Determination of the content of salidroside, privetin, calycosin glucoside and astragaloside IV in Zhenqi Fuzheng Granules by HPLC

[0071] Column Kromasil C 18 (4.6mm×250mm, 5μm), column temperature 30°C, flow rate: 1.0mL.min -1 .

[0072] Chromatography system Ⅰ: The mobile phase consists of methanol (A) and water (B). The gradient conditio...

Embodiment 2

[0095] 1.2 Moisture near-infrared indicators

[0096] 1.2.1 Modeling method

[0097] Collect 56 batches of finished products of Zhenqi Fuzheng Granules (sugar type), and use the standard method of "Chinese Pharmacopoeia" to measure their water content. Correlation model between the NIR spectrum of Fuzheng granules (with sugar) and the measured value of water content.

[0098] 1.2.2 Determination of moisture content in Zhenqi Fuzheng Granules by moisture method

[0099] Referring to the 2015 edition of "Chinese Pharmacopoeia", the fourth general rule 0832, the second method of drying, the specific method is as follows: take 2 to 5 g of the test product, spread it in a flat weighing bottle that has been dried to constant weight, and the thickness does not exceed 5mm. The test sample is no more than 10mm, accurately weighed, open the bottle cap and dry at 100-105°C for 5 hours, cover the bottle cap, move it to a desiccator, let it cool for 30 minutes, weigh it accurately, and t...

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Abstract

The invention relates to the field of near-infrared detection, in particular to an establishment method for a near-infrared quantitative correction model of Zhenqi Fuzheng Preparations and a detectionmethod for Zhenqi Fuzheng Preparations. The invention establishes NIR models of salidroside, calycosin-7-glucoside, Specnuezhenide, astragaloside A, and moisture index of Zhenqi Fuzheng Preparation finished products, which can simultaneously and quickly predict contents of multiple indexes of the Zhenqi Fuzheng Preparations without cumbersome sample processing, and prediction results are accurate, thereby satisfying detection requirements for Zhenqi Fuzheng Granule samples in the production. The method established in the invention not only shortens a detection period, reduces the use of consumables, and reduce detection costs of the Zhenqi Fuzheng Granules, but also enable on-site analysis. In the follow-up, the sample number will be further increased, the coverage of the samples will beexpanded, and a more stable model can be established to provide a fast and efficient detection method for the quality control of Zhenqi Fuzheng Granules.

Description

technical field [0001] The invention relates to the field of infrared detection, in particular to a method for constructing a near-infrared quantitative correction model of Zhenqi Fuzheng preparation and a detection method for Zhenqi Fuzheng preparation. Background technique [0002] Zhenqi Fuzheng is composed of Ligustrum lucidum and Astragalus membranaceus. It is recorded in "Prescriptions of Traditional Chinese Medicine" (Volume 20). It has the functions of improving human immunity and protecting bone marrow and adrenal cortex. A large number of pharmaceutical research and clinical applications have proved that it can be used as a "star" drug for cancer adjuvant radiotherapy and chemotherapy. [0003] As a fast and non-destructive green analysis technology, near-infrared (NIR) spectroscopy has the characteristics of fast analysis, simple sample processing, and no consumption of reagents. In recent years, near-infrared spectroscopy, as an indirect analysis technique, has ...

Claims

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Application Information

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IPC IPC(8): G01N21/359G01N21/3563G01N30/02
CPCG01N21/3563G01N21/359G01N30/02
Inventor 姜文月边雨王美慧徐杰曲佳乐姜国栋陈泓高陆胡铭
Owner JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT
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