Detection method of giant knotweed rhizome and glossy privet gout capsules

A Huzhen gout capsule and detection method technology, applied in the field of medicine, can solve the problems of long detection time, cumbersome operation, high detection cost, etc., and achieve the effects of high sensitivity, high quality and simplified operation steps

Active Publication Date: 2013-06-05
广东天键医药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the current quality standard of the drug is to use different chromatographic conditions to determine the content of one of the components, which has the disadvantages of cumbersome operation, long detec...

Method used

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  • Detection method of giant knotweed rhizome and glossy privet gout capsules
  • Detection method of giant knotweed rhizome and glossy privet gout capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Detection method of Huzhen gout capsule

[0028] Determination with high performance liquid chromatography, specifically comprises the following steps:

[0029] 1) Chromatographic conditions: use octadecylsilane bonded silica gel as filler, acetonitrile as mobile phase A, 0.1% formic acid solution as mobile phase B, carry out gradient elution as specified in the table below; detection wavelength is 280nm , the flow rate is 1.0ml / min, and the number of theoretical plates should not be less than 3000 according to the peaks of polydatin, psyllogenin, privetin and resveratrol;

[0030] time (minutes) Mobile phase A (%) mobile phase (B) 0 16 84 25 30 70 30 30 70

[0031] 2) Preparation of the reference substance solution: Take appropriate amounts of the polydatin reference substance, the psyllium reference substance, the privetin reference substance, and the resveratrol glycoside reference substance respectively, accurately wei...

Embodiment 2

[0054] Example 2 Determination of the content of different batches of Huzhen Tongfeng Capsules

[0055] Get the Huzhen Tongfeng Capsules of different batch numbers, measure according to the content determination method of embodiment 1, measure its result and see Table 1:

[0056] Table 1 The content test results of the main components of 3 batches of Huzhen Tongfeng Capsules

[0057] Huzhen Gout Capsules Polydatin content (%) Great psylloid content (%) The content of privetin (%) Resveratrol content (%) 120301 1.27 0.19 2.46 0.12% 120302 1.08 0.22 1.85 0.15 120501 1.12 0.17 2.14 0.12

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Abstract

The invention discloses a detection method of giant knotweed rhizome and glossy privet gout capsules. According to the invention, contents of polydatin, plantamajoside, specnuezhenide, and polydatin are determined by using a high performance liquid chromatography method. The method comprises the steps of sample solution preparation, control solution preparation, and determination. With the detection method provided by the invention, the contents of the four components of polydatin, plantamajoside, specnuezhenide, and polydatin can be simultaneously detected under a same chromatographic condition, such that operation steps of content determinations of the four components can be effectively simplified, determination time can be reduced, working efficiency can be improved, organic solvent application can be reduced, and effective control over the quality of giant knotweed rhizome and glossy privet gout capsules can be realized. The detection method has high sensitivity, good separation degree, and specificity. Accuracy, reproducibility, linear relationship, and stability of the method reach requirements of scientific researches and productions, such that the method can be popularized.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a detection method of Huzhen gout capsule. Background technique [0002] Huzhen gout capsule is a kind of medicine for treating gouty arthritis. The medicine has the effects of clearing away heat and promoting dampness, removing blood stasis and removing turbidity, and nourishing the liver and kidney. It is mainly used for primary acute gouty arthritis caused by damp-heat accumulation. The preparation method of Huzhen gout capsule is to use ethanol as a solvent, extract and concentrate, add appropriate amount of auxiliary materials to dry and then fill. Polydatin and resveratrol glycosides in Polygonum cuspidatum, privetin in Ligustrum lucidum, and psyllogenin in plantain are the main contained indicators. At present, the current quality standard of the drug is to use different chromatographic conditions to measure the content of one of the components respectively,...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 王一飞王治平李久香樊化杨珂
Owner 广东天键医药股份有限公司
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