Thin layer chromatographic detection method for traditional Chinese medicine composition for improving immunity of animals

Abstract

The invention discloses a thin layer chromatographic detection method for a traditional Chinese medicine composition. The thin layer chromatographic detection method comprises the following steps: a, respectively preparing a solution for test products; b, preparing a reference solution; c, applying a sample of each of the solution for test products, the reference solution of calycosin-7-glucoside and the reference solution of specnuezhenide on a same GF254 thin layer plate, developing by using chloroform-methanol-water as a developing solvent, taking out and then air drying; d, placing under an ultraviolet lamp (254nm) for inspection. According to the thin layer chromatographic detection method for the traditional Chinese medicine composition for improving immunity of animals, provided by the invention, astragalus membranaceus and fructus ligustri lucidi in the traditional Chinese medicine composition are both detected simultaneously, the detection result is accurate and reliable, and at the same time, the thin layer chromatographic detection method has the advantages of less process, high convenience in operation, short detection time and low cost and the like, and is particularly suitable for industrial application.

Description

technical field

[0001] The invention relates to a thin-layer chromatographic detection method of a traditional Chinese medicine composition for improving animal immunity. Background technique

[0002] Thin-layer chromatography (method) is a commonly used identification method for traditional Chinese medicinal materials, which has the advantages of simple equipment, easy operation, fast separation speed, high sensitivity and resolution.

[0003] Astragalus, also known as Astragalus, is the dried root of the leguminous plant Astragalus mongolica. It has the functions of nourishing qi and strengthening the surface, diuretic and swelling, detoxification and pus drainage, and muscle regeneration. It is a traditional Chinese medicine. The chemical composition of Radix Astragali is complex, mainly including saponins, flavonoids and their glycosides, polysaccharides, amino acids and so on. Ligustrum lucidum is the dry and mature fruit of Ligustrum lucidum, a plant belonging to the ...

Examples

Embodiment 1

[0027] Embodiment 1, the TLC detection method of Chinese medicine composition of the present invention

[0028] 1. Preparation of traditional Chinese medicine composition

[0029] In parts by weight: take 4 parts of Astragalus membranaceus and 1 part of Ligustrum lucidum, mix them, and grind them to 80 mesh with a universal pulverizer to obtain a coarse powder of the medicinal material, and then use a superfine pulverizer to grind the coarse powder of the medicinal material at a temperature of -10° C. It is obtained by pulverizing at 20°C until the drug particle size is 100-800 mesh. Chinese medicine composition.

[0030] 2. Detection method

[0031] Accurately weigh 2.0 g of the powder of the above-mentioned Chinese medicine composition, place it in a conical flask, add 40 ml of methanol, ultrasonically treat it for 60 min, filter, and get the supernatant; the supernatant is filtered with a 0.45 μm microporous membrane (PTFE), placed in Evaporate to dryness in an evaporati...

technical field

[0001] The invention relates to a thin-layer chromatographic detection method of a traditional Chinese medicine composition for improving animal immunity. Background technique

[0002] Thin-layer chromatography (method) is a commonly used identification method for traditional Chinese medicinal materials, which has the advantages of simple equipment, easy operation, fast separation speed, high sensitivity and resolution.

[0003] Astragalus, also known as Astragalus, is the dried root of the leguminous plant Astragalus mongolica. It has the functions of nourishing qi and strengthening the surface, diuretic and swelling, detoxification and pus drainage, and muscle regeneration. It is a traditional Chinese medicine. The chemical composition of Radix Astragali is complex, mainly including saponins, flavonoids and their glycosides, polysaccharides, amino acids and so on. Ligustrum lucidum is the dry and mature fruit of Ligustrum lucidum, a plant belonging to the ...

Examples

Embodiment 1

[0027] Embodiment 1, the TLC detection method of Chinese medicine composition of the present invention

[0028] 1. Preparation of traditional Chinese medicine composition

[0029] In parts by weight: take 4 parts of Astragalus membranaceus and 1 part of Ligustrum lucidum, mix them, and grind them to 80 mesh with a universal pulverizer to obtain a coarse powder of the medicinal material, and then use a superfine pulverizer to grind the coarse powder of the medicinal material at a temperature of -10° C. It is obtained by pulverizing at 20°C until the drug particle size is 100-800 mesh. Chinese medicine composition.

[0030] 2. Detection method

[0031] Accurately weigh 2.0 g of the powder of the above-mentioned Chinese medicine composition, place it in a conical flask, add 40 ml of methanol, ultrasonically treat it for 60 min, filter, and get the supernatant; the supernatant is filtered with a 0.45 μm microporous membrane (PTFE), placed in Evaporate to dryness in an evaporati...

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