Thin layer chromatographic detection method for traditional Chinese medicine composition for improving immunity of animals

Abstract

The invention discloses a thin layer chromatographic detection method for a traditional Chinese medicine composition. The thin layer chromatographic detection method comprises the following steps: a, respectively preparing a solution for test products; b, preparing a reference solution; c, applying a sample of each of the solution for test products, the reference solution of calycosin-7-glucoside and the reference solution of specnuezhenide on a same GF254 thin layer plate, developing by using chloroform-methanol-water as a developing solvent, taking out and then air drying; d, placing under an ultraviolet lamp (254nm) for inspection. According to the thin layer chromatographic detection method for the traditional Chinese medicine composition for improving immunity of animals, provided by the invention, astragalus membranaceus and fructus ligustri lucidi in the traditional Chinese medicine composition are both detected simultaneously, the detection result is accurate and reliable, and at the same time, the thin layer chromatographic detection method has the advantages of less process, high convenience in operation, short detection time and low cost and the like, and is particularly suitable for industrial application.

Description

technical field [0001] The invention relates to a thin-layer chromatographic detection method of a traditional Chinese medicine composition for improving animal immunity. Background technique [0002] Thin-layer chromatography (method) is a commonly used identification method for traditional Chinese medicinal materials, which has the advantages of simple equipment, easy operation, fast separation speed, high sensitivity and resolution. [0003] Astragalus, also known as Astragalus, is the dried root of the leguminous plant Astragalus mongolica. It has the functions of nourishing qi and strengthening the surface, diuretic and swelling, detoxification and pus drainage, and muscle regeneration. It is a traditional Chinese medicine. The chemical composition of Radix Astragali is complex, mainly including saponins, flavonoids and their glycosides, polysaccharides, amino acids and so on. Ligustrum lucidum is the dry and mature fruit of Ligustrum lucidum, a plant belonging to the ...

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Problems solved by technology

[0004] In the prior art, medicinal materials such as Radix Astragali, Ligustrum lucidum and their compositions are usually identified by thin-layer chromatography. However, the existing identification methods can only qualitatively identify one medicinal material in Astragalus and Ligustrum lucidum, such as: Liu Xiaoqing et al. (Liu Xiaoqing, Li Jun, Hu Jinglian, Li Xiaoyan, Qiming Kang Tingguo. Rapid identification of calycosin glucoside in Astragalus medicinal materials by TLC. Journal of Liaoning University of Traditional Chinese Medicine, July 2013, Volume 15, Issue 7.) through specific previous Treat and prepare the need testing solution, and select specific chloroform-methanol-water (7.5:2.5:0.2) as developing agent, just can obtain the detection result that thin-layer spot is clear, separation degree is better; Zhou Yang etc. (Zhou Yangliu Li Xu Desheng. Thin-layer chromatographic identification of Ligustrum lucidum bitter in decoction pieces of Ligustrum lucidum. China Pharmaceutical Industry, October 5, 2013, Volume 22, Issue 19.) The test solution was prepared by specific pretreatment, And choose a specific ethyl acetate-acetone-formic acid-water (6:3:1:1) as the developer, in order to obtain the test results with clear thin-layer spots and good resolution

[0005] Chinese literature (Zhai Shaoqin, Cao Guowen, Li Chengjun. TLC identification of compound golden granules. Journal of Chinese Veterinary Medicine, 2010, No. 6.) discloses the thin-layer identification method of compound golden granules, which requires the identification of astragalus, Ligustrum lucidum, etc. Different pretreatments were used to prepare the test solution, different developing agents were used, and thin-layer chromatography was carried out respectively. This method cannot carry out pretreatment and thin-layer chromatography on components such as Astragalus membranaceus and Ligustrum lucidum in compound granules at the same time. Inspection, there are many defects such as many procedures, complicated operation, long inspection time and high cost

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