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Equol-producing bacterium and use thereof

A technology of equol and bacteria, applied in the field of equol-producing bacteria, can solve problems such as low transformation ability, and achieve the effect of high-efficiency production

Active Publication Date: 2012-01-11
YAKULT HONSHA KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since the microorganisms reported above have a low conversion ability from daidzein as a substrate to equol, they cannot be used for the production of equol in the human body using microorganisms and the industrial production of equol using microorganisms.

Method used

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  • Equol-producing bacterium and use thereof
  • Equol-producing bacterium and use thereof
  • Equol-producing bacterium and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1 Production of equol

[0105] In 10L sorbose-PY medium containing daidzein (daidzein 0.025%, sorbose 1%, peptone 0.5%, tryptone 0.5%, yeast extract 1%, hemin 0.00005%, vitamin K1 0.0001 %, L-cysteine ​​hydrochloride 0.05%, KH 2 PO 4 0.0006%, K 2 HPO 4 0.0006%, NaCl 0.0012%, (NH 4 ) 2 SO 4 0.0006%, CaCl 2 0.00006%, MgSO 4 0.00006%), add 10 8 Pieces / medium·mL Slackia sp.YIT 11861, at 37℃, 96h, mixed gas (N 2 : H 2 : CO 2 =88:7:5) Incubate in the presence of. The culture solution was centrifuged (8000×g, 15 minutes), and the same amount of ether was added to the supernatant. After sufficiently stirring, it was separated into two layers by centrifugal separation (1000×g, 2 minutes), and only the diethyl ether layer was separated. The diethyl ether layer was dried at 40°C under a nitrogen stream to obtain 2.4 g of equol.

Embodiment 2

[0106] Example 2 Manufacture of tablets

[0107] The various ingredients were mixed with the formula in the following Table 4, granulated, dried, and sized, and then beaten to produce tablets.

[0108] [Table 4]

[0109]

[0110] 1) Manufactured by freeze-drying the live cells of Slackia sp.YIT 11861 (containing 10 live cells 10 Pcs / g).

Embodiment 3

[0111] Example 3 Manufacture of soft drinks

[0112] According to the formula in Table 5 below, the ingredients were blended in a conventional method and homogenized to obtain a refreshing drink. After the obtained soft drink was filled in a brown bottle, it was sealed with an aluminum cap, and heat treatment was performed.

[0113] [table 5]

[0114]

[0115] 1) Manufactured by freeze-drying the live cells of Slackia sp.YIT 11861 (containing 10 live cells 10 Pcs / g).

[0116]

[0117]

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PUM

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Abstract

Disclosed is microbe with the capacity to convert 50% or more of daidzein to equol in 24 hours, a food or medical composition that contains said microbe, a method of manufacturing equol using said microbe, and a nucleic acid fragment that can specifically detect said microbe.

Description

Technical field [0001] The invention relates to equol-producing bacteria and its utilization. Background technique [0002] The isoflavones contained in soy foods are effective in improving menopausal symptoms such as unidentified complaints (indeterminate complaints), prevention of osteoporosis, prevention of hyperlipidemia and arteriosclerosis, prevention of breast and prostate cancer, etc. The functional ingredients are widely known. According to recent studies, it is known that Daidzein, one of the isoflavones, is metabolized by the intestinal bacteria in the body into Equol, which has a stronger estrogen effect or a stronger antioxidant effect. Equol acts as a function in the body. One of the main effective ingredients of the above effects has attracted much attention. [0003] It has been reported that the production of daidzein to equol in the body is not the same in all people, and there are individual differences in its production ability. 30-50% of people have the abili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/30A61K31/7048A61K35/74A61K47/10A61K47/20C12N15/09C12P17/06C12R1/01
CPCC12P17/06A61K35/74C12R1/01A61K31/7048A23L1/3002A23L33/105A61P3/06A61P9/10A61P15/00A61P19/10A61P35/00C12N1/205C12R2001/01C12N1/20C12N15/11
Inventor 辻浩和野本康二森山熏赤座英之
Owner YAKULT HONSHA KK
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