Application method of beta-glucosidase in bean milk

A technology of glucosidase and application method, which is applied in the application field of β-glucosidase in soybean milk, can solve the problem of low bioavailability of isoflavones, improve bioavailability, facilitate digestion and absorption, and promote intestinal health Effect

Inactive Publication Date: 2017-11-03
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of isoflavone glucoside compounds in soybean is much higher than that of isoflavone aglycone compounds, so the bioavailability of isoflavones in natural soybean and its products is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Homemade soybean milk: Soak the soybeans in distilled water overnight; remove the soybean hulls and wash them with distilled water; take 200g of peeled soybeans and add 500mL of distilled water to grind them with a soybean milk machine; Filter to remove the insoluble residue; put the filtered soybean milk into the Erlenmeyer flask, sterilize at 121°C for 15 minutes; cool to room temperature, add filter-sterilized sucrose solution, and take samples to determine daidzin, genistin, and daidzin in the initial soybean milk element and genistein, and the rest were stored in a 4°C refrigerator for later use.

[0023] Strain cultivation: Take 300 μL of Lactobacillus plantarum cultured in a glycerol tube, inoculate it in MRS medium, cover the cap of the anaerobic bottle, and culture at 37°C for 20 hours. Fermentation culture: Inoculate the seed liquid of Lactobacillus plantarum into the prepared soybean milk medium, the inoculum amount is 4% (v / v), cultivate at 37°C for 12h, and...

Embodiment 2

[0029]Homemade soybean milk: Soak the soybeans in distilled water overnight; remove the soybean hulls and wash them with distilled water; take 200g of peeled soybeans and add 500mL of distilled water to grind them with a soybean milk machine; Filter to remove the insoluble residue; add the filtered soybean milk to the Erlenmeyer flask, sterilize under high pressure at 121°C for 15 minutes; cool to room temperature, add the filtered sterilized sucrose solution to make the concentration 2% (w / v), and take a sample for determination The content of daidzin, genistin, daidzein and genistein in the initial soybean milk, and the rest were stored in the refrigerator at 4°C for later use.

[0030] Strain cultivation: Take 300 μL of Lactobacillus plantarum LYT-3 bacterial solution stored in a glycerol tube, inoculate it in MRS medium, cover the anaerobic bottle cap, and culture at 37°C for 20 hours.

[0031] Fermentation culture: Inoculate the seed liquid of Lactobacillus plantarum LYT-...

Embodiment 3

[0037] Homemade soybean milk: Soak the soybeans in distilled water overnight; remove the soybean hulls and wash them with distilled water; take 200g of peeled soybeans and add 500mL of distilled water to grind them with a soybean milk machine; Filter to remove the insoluble residue; add the filtered soybean milk to the Erlenmeyer flask, sterilize under high pressure at 121°C for 15 minutes; cool to room temperature, add the filtered sterilized sucrose solution to make the concentration 2% (w / v), and take a sample for determination The content of daidzin, genistin, daidzein and genistein in the initial soybean milk, and the rest were stored in the refrigerator at 4°C for later use.

[0038] Strain cultivation: Take 300 μL of Lactobacillus plantarum LYT-3 bacterial solution stored in a glycerol tube, inoculate it in MRS medium, cover the anaerobic bottle cap, and culture at 37°C for 20 hours.

[0039] Fermentation culture: Inoculate the seed liquid of Lactobacillus plantarum LYT...

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PUM

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Abstract

The invention discloses an application method of beta-glucosidase in bean milk. Specifically, the bean milk is inoculated with cultured lactobacillus plantarum, and an isoflavone glucoside compound in the bean milk is directly converted into an isoflavone aglycon compound through the beta-glucosidase produced by the lactobacillus plantarum in the fermentation process of the bean milk, so that the biological availability of the bean milk is improved. After the application method disclosed by the invention is used, the highest activity of the beta-glucosidase is 9.24U / mL, the pH of the beta-glucosidase is 4.12, the content of lactic acid is 92.45mM, the content of acetic acid is 10.01mM, the content of soybean aglycone is 9.06mg / L and is increased by 1.9 times, and the content of genistein is 34.14mg / L and increased by 4.0 times. According to the application method disclosed by the invention, the biological availability of the bean milk is effectively improved, and the digestion and the absorption of human bodies are facilitated; the flavor of the bean milk is adjusted through the lactic acid and the acetic acid; and besides, through the addition of probiotics namely the lactobacillus plantarum, the health of the intestinal tracts of the human bodies is promoted.

Description

technical field [0001] The present invention relates to the application of β-glucosidase in soybean milk. Background technique [0002] Soymilk and soy flour are traditional soybean products with a long history of development. In the 1970s, it became popular in Asian countries such as China, Japan, and South Korea. Soy milk and soy flour are widely recognized because they are rich in soy protein and unsaturated fatty acids, and are free of lactose and cholesterol. Soybean is the main crop in our country, and the soybean processing industry is in the process of continuous development. At present, there are about 90 large and medium-sized processing enterprises producing soybean milk and soybean powder in China, with an annual output of 850,000 tons, 650,000 tons of processed soybeans, 350,000 tons of reconstituted soybean powder and soybean milk powder, and 500,000 tons of drinking soybean milk. It accounts for about 18% of soybean protein products. In 2013, the consumpti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C11/10A23L11/65
CPCA23C11/106
Inventor 李悦明梁荣荣李霞徐建春
Owner QINGDAO KEHAI BIOLOGICAL
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