Culture method capable of improving enzymatic activity of laccase

A culture method and technology of laccase enzyme activity, applied in the field of microorganisms, can solve the problems such as unsuitable acquisition of laccase fermentation culture bacteria

Inactive Publication Date: 2017-04-26
CHANGSHU JINDA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of low laccase enzyme activity and unfavorable acquisition of laccase ferme...

Method used

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  • Culture method capable of improving enzymatic activity of laccase
  • Culture method capable of improving enzymatic activity of laccase
  • Culture method capable of improving enzymatic activity of laccase

Examples

Experimental program
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Effect test

Embodiment 1

[0020] In this example, simultaneous inoculation was adopted for culturing for 3 days, and then the enzyme activity of laccase was determined according to the above method. And adopt oyster mushroom, Pleurotus osmanthus or Coprinus comatus to cultivate separately as a control, and the culture mode of simultaneous inoculation is cultivated according to the volume ratio in the following table 1, and the results of laccase activity are shown in table 1:

[0021] Table 1 laccase activity assay results

[0022]

Embodiment 2

[0024] Sequential inoculation (3 days after the inoculation of Pleurotus ostreatus, add Pleurotus osmanthus or Coprinus comatus for 3 days. The sequential inoculation culture method is carried out according to the volume ratio in Table 2 below. The results of laccase bacteria activity are shown in Table 2.

[0025] Table 2 laccase activity assay results

[0026]

Embodiment 3

[0028] Sequential inoculation (1-3 days after inoculation with Pleurotus ostreatus, inoculation with 10% Pleurotus osmanthus or Coprinus comatus for 3 days), the results of laccase activity are shown in Table 3.

[0029] Table 3 laccase activity assay results

[0030]

[0031] The above examples show that the present invention adopts Pleurotus and Pleurotus or Pleurotus and Agaricaceae Coprinus to carry out mixed culture, compared with a single bacterial species, the laccase activity is greatly improved. After cultivation, the activity of laccase was greatly improved, and unexpected technical effects were obtained.

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Abstract

The invention belongs to the field of microbial technology, and in particular relates to a culture method capable of improving enzymatic activity of laccase. Specifically, the method capable of improving the enzymatic activity of the laccase provided by the invention is achieved through mixed culture of pleurotus of pleurotaceae family and pleurotus or coprinus of agaricaceae family. According to the culture method provided by the invention, by conducting the mixed culture on the two bacteria and through simultaneous inoculation or ordered inoculation, the enzymatic activity of the laccase can be improved by 20-600%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a cultivation method for improving laccase enzyme activity. Background technique [0002] Laccase has a wide range of applications. It can not only catalyze and oxidize a variety of aromatic compounds, but also degrade lignin, remove the toxicity of many toxic phenolic substances, and decolorize a variety of dyes and remove the toxicity of petroleum industry wastewater. Therefore, It has great application value in textile printing and dyeing, paper making, lawn restoration, energy regeneration, feed, food, wastewater treatment and other fields. [0003] A variety of laccase-producing Pleurotus fungi have been found, such as Pleurotus pectinosa, Pleurotus erythrae, Pleurotus rotundum, etc., but the laccase activity is generally not high. At present, the improvement of laccase activity is mostly through the selection of strains with high laccase activity, adding...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/02
CPCC12N1/14C12N9/0061C12Y110/03002
Inventor 杨智钧王锦锦刘丹丹张友源卞兆祥吕爱平
Owner CHANGSHU JINDA TECH CO LTD
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