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Preparation method for lignocellulose decomposition composite flora and application thereof

A technology of complex flora and wood fiber, applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low total degradation rate of cellulose, long fermentation cycle, weak decomposition ability, etc. To achieve the effect of effective utilization, short cycle and high degradation rate

Inactive Publication Date: 2011-02-16
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method and application of a lignofiber-decomposing composite flora, in order to solve the problem of single type of microbial agent used in the straw degradation process, weak decomposition ability, low total degradation rate of cellulose and Long fermentation cycle and other issues, providing a high-efficiency straw decomposing compound flora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 The preparation method of lignocellulosic decomposition composite flora of the present invention:

[0016] Prepare according to the following specific steps:

[0017] (1) Preparation of cow dung medium: Weigh 150 g of cow dung and add it to a 1000 mL beaker, pour 400 mL of water into it, stir for 30 minutes, filter to remove the slag, take 100 mL and add it to a 250 mL Erlenmeyer flask for later use;

[0018] (2) Shake flask culture inoculum preparation: get 40g of cow manure compost, sieve, then weigh 20g and add it to a 1000mL beaker containing 50mL of water, fully stir for 30min, and set aside;

[0019] (3) Take 10mL shake flask culture inoculum and add it to a 250mL Erlenmeyer flask with 100mL liquid cow dung medium, and place it on a shaker with a rotation speed of 120r / min and a temperature of 45°C for 3d to 5d;

[0020] (4) Cultivation of Phanerochaete chrysosporium and Trichoderma sp, Rhizopus sp, Panaeolus sp, Coprinus sp isolated from cow dung by...

Embodiment 2

[0022] The fermented liquid that the fermentation of embodiment 2 cow dung culture medium obtains and the trichoderma (Trichoderma sp), rhizopus sp (Rhizopus sp), rhizopus sp. The spore suspension mixture of Panaeolus sp and Coprinus sp was prepared as in Example 1.

[0023] The composite microbial agent is suitable for composting with straw and manure as main raw materials.

Embodiment 3

[0024] The fermented liquid obtained by the fermentation of embodiment 3 cow dung culture medium and Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the Trichoderma sp (Trichoderma sp) and Rhizopus sp (Rhizopus sp) isolated from cow dung using the conventional method of microorganism isolation The preparation of the spore suspension mixture was as in Example 1.

[0025] The fermented liquid obtained by the fermentation of the cow dung medium is mixed with the spore suspension of Phanerochaete chrysosporium (Phanerochaete chrysosporium), Trichoderma (Trichoderma) and Rhizopus sp. The volume ratio of the three bacterial solutions is 1:2:1. The composite bacterial agent is suitable for composting with straw (the volume ratio of the bacterial liquid and the crushed straw of about 1 cm is 1:100-1:200, the two are fully mixed, and the humidity is controlled at 75-80%) as the main raw material for composting.

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Abstract

The invention relates to a preparation method for a lignocellulose decomposition composite flora and application thereof, discloses a method which can prepare a high-efficiency lignocellulose decomposition composite flora rapidly, simply and conveniently, in particular provides a microbial inoculum for the composting of cow muck and agricultural straw, and solves the problem that the cow muck and the agricultural straw are difficult to compost. The composite flora is formed by mixing fermentation liquor obtained by fermenting a cow muck culture medium and spore suspension of phanerochete chrysosporium and trichoderma sp, rhizopus sp, panaeolus sp and coprinus sp which are separated from the cow muck, and can completely degrade the cow muck and the agricultural straw of lignocellulose. By using the microbial inoculum in the composting of the cow muck and the straw, the fermentation period is as short as only 25 days. The preparation method for the flora is simple and is easy to implement; the flora content in each unit of raw materials is high; and the activity is high.

Description

technical field [0001] The invention relates to a preparation method and application of a lignofiber-decomposing composite flora, and is a biological technology for treating agricultural waste straws by using microorganisms. technical background [0002] my country is a big country for raising cattle. In recent years, with the development of the cattle raising industry in the direction of scale and intensification, the pollution of cow dung to the environment has become more and more serious. The 1999 China Agricultural Statistical Yearbook shows that the number of cattle raised is 35.0694 million heads per year, and the manure produced by each cow is 10.6 tons per year, and the urine production is 4.9 tons per year; the total manure produced is 136.8952 million tons per year, and the total output The urine output is 45.0066 million tons per year. The composting method takes three to four months or even half a year for cold cow dung, which takes a long time and has low proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/885C12N1/00C12N1/14C05F11/08C12R1/66C05F17/00C12R1/845C12R1/645
CPCY02W30/40
Inventor 宁平周成刘张黄兆林瞿广非熊向峰邓志华黄凯贾丽娟
Owner KUNMING UNIV OF SCI & TECH
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