Breeding method for kumquat triploid

A technology of triploid and kumquat, which is applied in the field of kumquat triploid breeding, can solve the problems of high technical requirements, difficulty in obtaining triploid, poor growth of regenerated plants, etc., and achieve short breeding period, elimination of interference, and simple operation Effect

Active Publication Date: 2015-04-08
GUANGXI UNIV
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Problems solved by technology

[0006] The way to obtain triploids in the prior art usually adopts sexual hybridization and endosperm cultivation of tetraploids and diploids. Among them, it is difficult to obtain triploids for polyembryonic fruit trees due to the interference of n

Method used

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Embodiment

[0033] The present invention uses kumquat fresh-eating cultivar "Yangshuo kumquat" as the mutagenesis material. Specific steps are as follows:

[0034] (1) Tetraploid induction: Select the plump seeds of the mature fruit of Yangshuo kumquat, remove the outer seed coat, peel off the inner seed coat after soaking for 5 hours, separate the seed embryo, and then soak it in a 0.2% concentration of colchicine solution 36h;

[0035] (2) Germination and cultivation of mutagenized seeds: take out the soaked seeds, and after cleaning, germinate and germinate roots in a light incubator, keep moisture, and observe the germination situation regularly. After rooting, 1 cm of root tips were cut and placed in Carnot's fixative solution (95% ethanol: glacial acetic acid = 3:1) to fix, numbered sequentially, and used for chromosome ploidy detection;

[0036] (3) Chromosomal ploidy check;

[0037] (4) Cultivation of tetraploid plants: the number of the seed embryos whose inspection result is ...

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Abstract

The invention discloses a breeding method for kumquat triploid. The method comprises the following steps: tetraploid mutagenesis, sprouting cultivation of seeds after mutagenesis, chromosome ploidy inspection, cultivation of tetraploid plants, tetraploid fruit harvesting and seed extraction, tetraploid embryo separation and cultivation, embryo chromosome detection, and cultivation of triploid seedlings. According to the method, a sexual hybridization process is avoided; the interference of nucellar embryo is eliminated; the triploid is directly separated and cultivated from polyembryonic tetraploid seeds; and the method has the advantages of being simple to operate, and short in breeding period.

Description

technical field [0001] The invention belongs to the technical field of fruit tree genetics and breeding, and in particular relates to a breeding method of kumquat triploid. Background technique [0002] Kumquat is a plant of the genus Fortunella Swingle in the subfamily Aurantioideae of the family Rutaceae. There are 6 species and 1 variety in the genus Kumquat. Kumquat is a general term for cultivated species. It can bloom and bear fruit many times a year. The fruit is beautiful in color and shape, rich in nutrition, and has the meaning of auspicious and festive. It is a multi-purpose fruit tree for fresh eating, processing and viewing. All species of kumquat are native to my country. However, there are few varieties of kumquat cultivated at present, and there is only one type of fresh food. Yangshuo kumquat is a representative fresh food variety with a large cultivation area and good economic benefits. [0003] Polyploid breeding is an important method for fruit tree bre...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/08
Inventor 黄桂香张树伟
Owner GUANGXI UNIV
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