Breeding method for kumquat triploid
A technology of triploid and kumquat, which is applied in the field of kumquat triploid breeding, can solve the problems of high technical requirements, difficulty in obtaining triploid, poor growth of regenerated plants, etc., and achieve short breeding period, elimination of interference, and simple operation Effect
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[0033] The present invention uses kumquat fresh-eating cultivar "Yangshuo kumquat" as the mutagenesis material. Specific steps are as follows:
[0034] (1) Tetraploid induction: Select the plump seeds of the mature fruit of Yangshuo kumquat, remove the outer seed coat, peel off the inner seed coat after soaking for 5 hours, separate the seed embryo, and then soak it in a 0.2% concentration of colchicine solution 36h;
[0035] (2) Germination and cultivation of mutagenized seeds: take out the soaked seeds, and after cleaning, germinate and germinate roots in a light incubator, keep moisture, and observe the germination situation regularly. After rooting, 1 cm of root tips were cut and placed in Carnot's fixative solution (95% ethanol: glacial acetic acid = 3:1) to fix, numbered sequentially, and used for chromosome ploidy detection;
[0036] (3) Chromosomal ploidy check;
[0037] (4) Cultivation of tetraploid plants: the number of the seed embryos whose inspection result is ...
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