Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala

A technology of distant hybridization and bream, applied in fish farming, application, climate change adaptation, etc., to achieve the effect of good traits, strong stress resistance and fast growth of offspring

Active Publication Date: 2015-05-06
湖南岳麓山水产育种科技有限公司
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Problems solved by technology

So far, there have been no reports of successful distant hybridization between carp and roundhead bream at home and abroad. It is of great applicatio

Method used

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  • Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala
  • Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala
  • Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala

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Embodiment

[0034] A method for distant hybridization between carp and bream subfamily of the present invention, comprising the following steps: firstly select female carp and male bream with obvious sexual maturity characteristics and good physical signs as broodstock special ponds for cultivation, and then in the breeding season Carry out artificial oxytocin to the parent fish, select the parent fish with good oxytocin effect to carry out artificial dry insemination, the embryos after insemination are hatched in running water in a clean water hatching device, and the fish fry are raised after hatching is completed, and then the reared fish fry are hatched. Detection and screening, through flow cytometry DNA content determination and kidney tissue chromosomal ploidy detection, the hybrid offspring of carp and roundhead bream were obtained. The hybrid offspring include diploid natural gynogenetic carp, diploid natural gynogenetic mirror carp, Diploid fish and tetraploid hybrid fish like cr...

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Abstract

A method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala includes the following steps that firstly, female cyprinus carpio and male megalobrama amblycephala which have obvious sexual maturity characteristics and good somatic features are selected as parent fishes for special pool breeding, then, artificial spawning induction is conducted on the parent finishes in a breeding season, the parent fishes with a good spawning induction effect are selected to be subjected to artificial dry-method fertilization, running water incubation is conducted on fertilized embryos in a pure water incubation device, fish fry are raised after being incubated, the raised fish fry are detected and screened, hybrid offspring of the cyprinus carpio and the megalobrama amblycephala are obtained through flow cytometry DNA content determination and renal tissue chromosome ploidy detection, and the hybrid offspring comprise diploid natural gynogenesis cyprinus carpio, diploid natural gynogenesis mirror carps, diploid fish like the cyprinus carpio and tetraploid hybrid fish. The method has the advantages that the rate of fertilization is high, the incubating rate is high, the offspring are excellent in character, and the method has great significance in genetic breeding.

Description

technical field [0001] The invention relates to a fish hybridization method, in particular to a distant hybridization method among subfamilies of carps. Background technique [0002] Content Determination The hybridization of genetic relationship between species or above is called distant hybridization, which is a commonly used method for fish trait improvement. As an important method of fish genetic breeding and trait improvement, distant hybridization can integrate a whole set of exogenous genes, thereby changing the expression regulation of hybrid progeny genes, so that hybrid progeny may improve in growth speed, stress resistance, disease resistance and Meat quality and other aspects showed heterosis. Distant hybridization can form different types of fish with different phenotypes and genotypes according to different combinations of parents. For example, F 1 -F 2 For diploid hybrid fish (2n=100), F 3 -F 24 It is an allotetraploid fish (4n=200); using allotetraploid...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 刘少军肖军王石赵如榕胡方舟陶敏张纯刘筠罗凯坤
Owner 湖南岳麓山水产育种科技有限公司
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