Construction method of portunus trituberculatus miers disease-resistant variety

A technique for Portunus trituberculatus and its construction method, which is applied in the field of constructing disease-resistant strains of Portunus trituberculatus, can solve the problems of long time-consuming breeding, slow results, and large number of offspring, and achieve the purpose of speeding up the breeding process, alleviating inbreeding depression, genetic clear background effect

Active Publication Date: 2010-01-06
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection is very cumbersome and difficult to operate.
Group breeding takes a long time and yields slow results
In recent y

Method used

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  • Construction method of portunus trituberculatus miers disease-resistant variety

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Embodiment 1

[0016] The information of the mitochondrial control region sequence of the breeding material is used as a molecular marker, and the breeding material is a population of crabs from various regions. Using specific primers (upstream primer sequence 5'-AGGAATTAAGAAACAATCAAACTTT-3', downstream primer sequence 5'-GCCGTTGAGTGAAGAGTGTTCAGATAG-3'), the template is the genomic DNA of the selection material for PCR in vitro amplification. The total PCR reaction volume is 25uL, which contains 0.5ul of dNTP (10mM), 5uM 1ul of each primer, 0.4U Taq enzyme, 10×PCR Buffer2.5ul, 25mM Mgcl 2 1.5ul. The PCR reaction was performed on a Bio-rad MyCycler PCR instrument. PCR reaction program: 3 minutes at 95 degrees; then 30 seconds at 95 degrees, 30 seconds at 55 degrees, 1 minute at 72 degrees, 29 cycles; finally 7 minutes at 72 degrees. PCR products were purified by 1% agarose gel electrophoresis and gel recovery kit. Use ABI Prism 3100 DNAAnalyzer to perform one-way sequencing to obtain the ...

Embodiment 2

[0020] Location: Ninghai County, Zhejiang Province.

[0021] In August 2005, 50 female and male swimming crabs with mature gonads were imported into the Donghai sea area of ​​Ningbo Xiangshan Port in the Shuangpantu aquatic seedling farm of Ninghai County, Ningbo City, Zhejiang Province, and 1.0×10 10 Soak the swimming crab parent body in the vibrio alginolyticus bacteria solution of high concentration, wash the surviving individuals with clear water after 72 hours, and put them back into the holding pond for holding temporarily. The offspring produced by random mating were bred to adults.

[0022] In October 2006, the disease-resistant candidate populations that had been bred to adults in the previous year were subjected to the same infection treatment at the Deshui Aquatic Fry Farm in Ninghai County, Ningbo City, Zhejiang Province. At the same time, the Laizhou Bay population in Shandong Province and the Two genetically independent populations of Guangxi Beihai population w...

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Abstract

The invention relates to the aquatic product heredity and breeding field, in particular to a construction method of a portunus trituberculatus miers disease-resistant variety. The method comprises the following concrete steps: information of mitochondrial control zone sequence of breeding materials serves as a molecular marker, group with rich heredity mutation serves as base group of group breeding according to the molecular marker, and groups which are genetically independent serve as the supplementary materials of family breeding; first, group breeding is carried out on the base group to obtain group, family breeding is carried out on the obtained group and the supplementary materials of family breeding, then, interfamily hybridization combination is carried out on groups with stable disease-resistant character obtained from family breeding, and the family with stable disease-resistant character is the disease-resistant variety; wherein breeding materials in each step are individuals which survive with half lethal concentration after being soaked and infected by source of infection. The invention shortens the breeding time while making the most use of species genetic resources simultaneously, combines the adaptability of local crabs, and effectively avoids the inbreeding depression of small groups, thus facilitating scientifically cultivation of the portunus trituberculatus miers disease-resistant variety suitable for local aquaculture.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a method for constructing a disease-resistant strain of Portunus trituberculatus. Background technique [0002] The genetic breeding of aquatic animals is traditionally carried out by group selection or crossbreeding. At present, there are also attempts to learn from the method of family selection used in poultry storage. Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection and breeding are very cumbersome and difficult to operate. Group selection takes a long time and has slow results. In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but in practical applications, especially in the genetic breeding of aquatic animals, crabs have not yet achieved results. [0003] The present invention successfully develops a breeding method suitable for...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12N15/12
CPCY02A40/81
Inventor 崔朝霞李喜莲刘媛王鸿霞
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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