Method for breeding male sterility restoring line of yellow sprout pepper cytoplasm

A technology of male sterile lines and capsicum cytoplasm, applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the difficulty of screening large fruit type restorer line materials, consume manpower, material and financial resources, and have low efficiency, etc. problem, to achieve the effect of controllable breeding target traits and procedures, simple breeding procedures, and improved breeding efficiency

Active Publication Date: 2015-11-25
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Application Information

AI Technical Summary

Problems solved by technology

However, since the distribution of restorer line materials in different types of peppers is different, and the identification is mainly through a large number of paired side crosses, the workload is large and the efficiency is not high. In small-fruited peppers such as croissant and threa

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0021] Breeding of Restorer Line of Yahuang Pepper Cytoplasmic Male Sterile Line 140-6A

[0022] Step 1. Pollen collection: In the spring of 2011, the female parent was the cytoplasmic male sterile line 140-6A of bud yellow pepper. The fruit was cone-shaped and sweet. Taking 100 inbred peppers as the male parent as a group, there are 3 groups in total, and 300 male parent materials are divided into groups A, B and C, in which groups A and B are peppers, and group C is sweet peppers; For the collection of pollen, pollen is collected directly from the flower of the male parent that bloomed on the same day. Each male parent collects 2 flower pollen, and the pollen of 100 male parents is collected as a group, divided into 3 groups, and the collected male parent pollen Use a shaker to mix evenly, after numbering, directly pollinate;

[0023] Step 2, pollination: pollinate the sterile line 140-6A with mixed pollen of 3 groups of numbers, pollinate the flowers that are in full bloom...

Embodiment 2

[0029] Breeding of Restorer Line of Yahuang Pepper Cytoplasmic Male Sterile Line 140-2A

[0030]Step 1. Pollen collection: in the spring of 2011, the male parent of bud yellow pepper cytoplasmic male sterile line 140-2A (its genotype is S(rfrf)) was used as the female parent. Taking 50 inbred peppers as male parents (unknown genotype materials) as one group, there are 3 groups in total, 150 male parent materials, divided into groups A, B and C, wherein groups A and B are peppers, C The group is sweet pepper; the pollen collection of the male parent, the pollen is directly taken from the flower of the male parent in full bloom on the same day, and each male parent collects 2 flower pollens, and the pollen of 50 male parents is collected as a group, divided into 3 groups, The pollen collected from the male parent was mixed evenly with a vibrator, numbered, and directly pollinated;

[0031] Step 2. Pollination: pollinate the sterile line 140-2A with mixed pollen of three groups ...

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Abstract

The invention discloses a method for breeding the male sterility restoring line of yellow sprout pepper cytoplasm and belongs to the technical field of plant breeding. The method includes the specific steps that mixed pollination is conducted on the male sterility restoring line of the yellow sprout pepper cytoplasm and selfing line pepper male parent pollen, harvested hybrid seeds are sowed, the fertile plant line and fertile plants are screened many times, strict selfing is conducted, and the male sterility restoring line of the yellow sprout pepper cytoplasm with the restoring rate being 100% is obtained finally; compared with an existing breeding technology of the pepper male sterility restoring line, the method has the advantages that the method is simple in breeding procedure, convenient to implement and controllable in target trait and procedure of breeding; the breeding efficiency is improved greatly, the breeding restoring line type and number are increased greatly, and a new purpose is developed for advantage utilization of pepper crossbreed, leaf color marking sterility restoring line breeding and the like.

Description

technical field [0001] The invention relates to a method for breeding capsicum, in particular to a method for breeding a cytoplasmic male sterile restorer line of bud yellow capsicum, and belongs to the technical field of plant breeding. Background technique [0002] Studies have shown that there is obvious heterosis in hybrids among pepper varieties. The traditional ways to utilize the heterosis of pepper include interspecific hybridization with artificial castration, double cross, triple cross, male sterile line seed production, chemical castration seed production and so on. Breeding new varieties using male sterile dual-purpose lines and cytoplasmic male sterile lines has become the focus of pepper breeding. Since the capsicum cytoplasmic male sterile line can produce 100% sterile plants, it can greatly improve the purity of the first-generation hybrid seeds, making it of high production significance. However, since the distribution of restorer line materials in differe...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 马志虎孙国胜孙春青潘跃平
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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