Method and application method for obtaining novel germ plasm of brassica A genome vegetable

Abstract

The invention discloses a method and application method for obtaining a novel germ plasm of brassica A genome vegetable. In the method, free microspore culture is carried out on crossbreeds among vegetables of Chinese cabbage subspecies*pakchoi cabbage subspecies or variety in pakchoi cabbage subspecies*variety of the brassica A genome to obtain a regeneration plant and further to create the novel germ plasm which is used for breeding. The method mainly comprises the following steps of: hybridizing the vegetables of Chinese cabbage subspecies*pakchoi cabbage subspecies or variety in pakchoi cabbage subspecies*variety; inducing free microspore culture embryoids; germinating the embryoids to obtain the regeneration plant; carrying out ploidy identification on the regeneration plant obtainedby microspore culture; reserving seeds of double haploids and polyploids; planting an offspring of the regeneration plant to carry out morphological identification and nutritive index identification;and preparing, hybridizing and combining. The method has the advantages of short cycle and high efficiency, provides a material for efficiently breeding Chinese cabbage vegetables and has broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and haploid breeding, and in particular relates to a method for quickly obtaining new vegetable germplasm of Brassica A genome through a free microspore culture method and applying it to breeding. Background technique [0002] Brassica A genome (2n=2x=20) vegetable, divided into pakchoi subspecies and Chinese cabbage subspecies. , Leiyongcai, etc. This subspecies is characterized by open leaves, short plant type, and obvious petioles. Most of the leaves are smooth and hairless, without obvious leaf wings, and do not form obvious leaf balls. The subspecies of Chinese cabbage can be divided into loose-leaf varieties, semi-headed varieties, flower-centered varieties, and head-shaped varieties. They are characterized by larger plant types, obvious petioles and leaf wings, and many rough leaf surfaces. Form leaf balls. There are great differences in vegetable traits between subspecies and varie...

AI Technical Summary

Problems solved by technology

The advantages of different subspecies and varieties of vegetables can be combined through sexual hybridization. The hybrid offspring are highly heterogeneous in genotype, and the highly pure parents required in dominant hybrid breeding can be self-crossed and segregated by conventional methods. Continue self-breeding for several generations, labor-intensive and time-consuming

With the improvement of people's living standards, the traditional method of hybridization within varieties and between varieties is difficult to meet the requirements of high-yield, high-quality, and novel-shaped vegetables required by the market, and the hybrids between subspecies and within subspecies are free Microspore culture can quickly obtain double haploid pure lines, and the differences between the pure lines are very large, which provides a larger selection range, solves this problem, and meets the needs of breeding work

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