Method and application method for obtaining novel germ plasm of brassica A genome vegetable

A technology of Brassica and genome, applied in the field of biotechnology and haploid breeding, breeding, can solve problems that are difficult to meet, have large differences, time-consuming, etc., and achieve the effect of increasing probability, short cycle and high efficiency

Inactive Publication Date: 2011-08-10
SHENYANG AGRI UNIV
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Problems solved by technology

The advantages of different subspecies and varieties of vegetables can be combined through sexual hybridization. The hybrid offspring are highly heterogeneous in genotype, and the highly pure parents required in dominant hybrid breeding can be self-crossed and segregated by conventional methods. Continue self-breeding for several generations, labor-intensive and time-consuming
With the improvement of people's living standards, the traditional method of...

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  • Method and application method for obtaining novel germ plasm of brassica A genome vegetable
  • Method and application method for obtaining novel germ plasm of brassica A genome vegetable
  • Method and application method for obtaining novel germ plasm of brassica A genome vegetable

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Embodiment Construction

[0023] The method provided by the present invention for obtaining new vegetable germplasm of the Brassica genus A genome and applying it to breeding includes: the hybridization process of 'subspecies of Chinese cabbage x subspecies of pakchoi', 'variety of subspecies of pakchoi x variety', free microspores During the cultivation process, the ploidy identification of regenerated plants obtained from microspore culture, the morphological identification and nutritional index identification process of the offspring of planted regenerated plants in the process of saving seeds for double haploid and hyperploid, and the process of preparing hybrid combinations.

[0024] The specific method steps are as follows:

[0025] A. Brassica 'Chinese cabbage subspecies×Pakchoi subspecies' or 'Pakchoi subspecies subspecies×variety' hybridization process: According to the breeding goal, determine the parents, hybridize at the flowering stage, and obtain F 1 Seeds, planted F 1 , selfing to obtai...

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Abstract

The invention discloses a method and application method for obtaining a novel germ plasm of brassica A genome vegetable. In the method, free microspore culture is carried out on crossbreeds among vegetables of Chinese cabbage subspecies*pakchoi cabbage subspecies or variety in pakchoi cabbage subspecies*variety of the brassica A genome to obtain a regeneration plant and further to create the novel germ plasm which is used for breeding. The method mainly comprises the following steps of: hybridizing the vegetables of Chinese cabbage subspecies*pakchoi cabbage subspecies or variety in pakchoi cabbage subspecies*variety; inducing free microspore culture embryoids; germinating the embryoids to obtain the regeneration plant; carrying out ploidy identification on the regeneration plant obtainedby microspore culture; reserving seeds of double haploids and polyploids; planting an offspring of the regeneration plant to carry out morphological identification and nutritive index identification;and preparing, hybridizing and combining. The method has the advantages of short cycle and high efficiency, provides a material for efficiently breeding Chinese cabbage vegetables and has broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and haploid breeding, and in particular relates to a method for quickly obtaining new vegetable germplasm of Brassica A genome through a free microspore culture method and applying it to breeding. Background technique [0002] Brassica A genome (2n=2x=20) vegetable, divided into pakchoi subspecies and Chinese cabbage subspecies. , Leiyongcai, etc. This subspecies is characterized by open leaves, short plant type, and obvious petioles. Most of the leaves are smooth and hairless, without obvious leaf wings, and do not form obvious leaf balls. The subspecies of Chinese cabbage can be divided into loose-leaf varieties, semi-headed varieties, flower-centered varieties, and head-shaped varieties. They are characterized by larger plant types, obvious petioles and leaf wings, and many rough leaf surfaces. Form leaf balls. There are great differences in vegetable traits between subspecies and varie...

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Application Information

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IPC IPC(8): A01H4/00A01H5/12
Inventor 冯辉章云王爱杰刘洋刘志勇李承彧冀瑞琴王玉刚
Owner SHENYANG AGRI UNIV
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