Cymbidium edaphic orchids seed aseptic seeding growing seedlings method

Abstract

The invention provides a method of the aseptic seeding and culturing of the cymbidium aseptic seed, comprising the steps of aseptic seeding, aseptic seedling culturing and seedling hardening, which is characterized in that after the aseptic seeding of the seed, a low temperature and darkness pre-culture step is added. The invention aims at enabling the cymbidium aseptic seed to complete the after ripening process and to promote germination by using the low temperature and dark condition and simulating the proterozonic environment of the cymbidium aseptic seed. Compared with the prior art, the invention improves the average bourgeon rate of the aseptic seeding of the cymbidium aseptic seed by over 10 times and has the advantages of simpleness and practicality, short seed culturing period and convenience for a large-scale production and so on, and the invention lays a firm basis for the novel variety selection of the cymbidium aseptic crossbreed.

Description

technical field [0001] The invention relates to a method for aseptic sowing and raising seedlings of ground orchid seeds of the genus Orchid. Background technique [0002] According to the book "Chinese Orchid Plants" edited by Liu Zhongjian, Chen Xinqi, etc., there are about 68 species of Cymbidium plants in the world, which are divided into three types: terrestrial, epiphytic, and saprophytic according to their living habits. Among them, there are not many studies on saprophytic species. The research on tissue culture technology of epiphytic species such as Jasperus jasperi, Tiger head orchid, Daxuelan, and Huangchanlan was earlier at home and abroad, and the technology of aseptic sowing seedlings is relatively mature. The aseptic sowing technique of conventional Orchid epiphytic orchid seeds for ground species such as Lotus petal orchid, Douban orchid, Chunlan, and Chunjian can hardly germinate after sowing. Some improvements have been made, including inoculation after u...

AI Technical Summary

Problems solved by technology

The aseptic sowing technique of conventional Orchid epiphytic orchid seeds for ground species such as Lotus petal orchid, Douban orchid, Chunlan, and Chunjian can hardly germinate after sowing. Some improvements have been made, including ultrasonic vibration, NaOH or KOH soaking, cutting the seed coat, etc. before inoculation, improving the medium formula, optimizing the culture conditions, etc., but the germination rate of the seeds is often less than 1% or even lower.