Cymbidium edaphic orchids seed aseptic seeding growing seedlings method

A technology for aseptic seeding and seeding, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of inability to germinate seeds, insufficient seed germination rate, etc., achieve a short seedling cycle, facilitate large-scale production, and improve efficiency effect

Inactive Publication Date: 2008-02-20
云南省农业科学院高山经济植物研究所
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AI Technical Summary

Problems solved by technology

The aseptic sowing technique of conventional Orchid epiphytic orchid seeds for ground species such as Lotus petal orchid, Douban orchid, Chunlan, and Chunjian can hardly germinate after sowing. Some improvements have been made, includin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Sowing: Take the lotus petal orchid capsule of 7-month fruit age, cut off the residual perianth, columella and excessively long fruit stalk, wipe the surface with 75% alcohol, transfer it to the ultra-clean workbench, and use 0.1 %HgCl 2 Soak and sterilize in medium for 15 minutes, wash with sterile water 5 times, then cut open the capsule and take out the seeds, and spread them on the sowing medium. The rest of the ingredients remain unchanged, the same below)+6-BA0.1mg·L -1 +NAA 3mg·L -1 +Activated carbon 2g·L -1 +Coconut milk 100mL·L -1 ;

[0012] (2) Pre-culture: after sowing, place the seeds together with the culture container under constant temperature and dark conditions at 4°C for 3 months;

[0013] (3) Sterile seedling cultivation: after the pre-cultivation is completed, the seeds are moved into the light culture room together with the culture container for cultivation. After 3 months, the seeds germinate one after another. Rapid proliferation of rhiz...

Embodiment 2

[0016] Take the watercress orchid capsules of 6-month fruit age, put 2~8 ℃ of naturally variable temperature and dark conditions after seed aseptic sowing and cultivate for 5 months, other steps are carried out by the method for embodiment 1, and the seed germination rate of sampling statistics is 25.5%.

Embodiment 3

[0018] Take the 6.5-month-old Chunlan×Chunjian hybrid capsules, and cultivate the seeds for 4 months under constant temperature and dark conditions at 7°C after sowing. Other steps are carried out according to the method in Example 1, and the seed germination rate is 21.4% according to sampling statistics.

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Abstract

The invention provides a method of the aseptic seeding and culturing of the cymbidium aseptic seed, comprising the steps of aseptic seeding, aseptic seedling culturing and seedling hardening, which is characterized in that after the aseptic seeding of the seed, a low temperature and darkness pre-culture step is added. The invention aims at enabling the cymbidium aseptic seed to complete the after ripening process and to promote germination by using the low temperature and dark condition and simulating the proterozonic environment of the cymbidium aseptic seed. Compared with the prior art, the invention improves the average bourgeon rate of the aseptic seeding of the cymbidium aseptic seed by over 10 times and has the advantages of simpleness and practicality, short seed culturing period and convenience for a large-scale production and so on, and the invention lays a firm basis for the novel variety selection of the cymbidium aseptic crossbreed.

Description

technical field [0001] The invention relates to a method for aseptic sowing and raising seedlings of ground orchid seeds of the genus Orchid. Background technique [0002] According to the book "Chinese Orchid Plants" edited by Liu Zhongjian, Chen Xinqi, etc., there are about 68 species of Cymbidium plants in the world, which are divided into three types: terrestrial, epiphytic, and saprophytic according to their living habits. Among them, there are not many studies on saprophytic species. The research on tissue culture technology of epiphytic species such as Jasperus jasperi, Tiger head orchid, Daxuelan, and Huangchanlan was earlier at home and abroad, and the technology of aseptic sowing seedlings is relatively mature. The aseptic sowing technique of conventional Orchid epiphytic orchid seeds for ground species such as Lotus petal orchid, Douban orchid, Chunlan, and Chunjian can hardly germinate after sowing. Some improvements have been made, including inoculation after u...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 薛润光和寿星李兆光和加卫杨少华杨泽李燕
Owner 云南省农业科学院高山经济植物研究所
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