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Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon

A technology of male sterility and dual-purpose lines, applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problems of reduced heterosis, difficulty in controlling the scale of seed production, high cost, etc., and increase the maintenance time of heterosis , The effect of easy control of seed production scale, yield and quality assurance

Inactive Publication Date: 2008-02-20
四川省农业科学院经济作物育种栽培研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing GMS two-line method and the GMS secondary method for seed production all need to use artificial pollination, which requires a large amount of labor and high costs. The workload of artificial pollination is relatively large, which limits the production of cotton hybrid seeds and the promotion and utilization of hybrids. This is a difficult problem that needs to be overcome
In addition, there are the following problems in the hybrid seed production of the two-line male sterile method: 1. When preparing hybrids every year, it is necessary to sow the male sterile line and the restorer line parents, and it is not possible to sow seeds for multiple harvests at one time. To remove sterile plants and miscellaneous plants, seed production is labor-intensive and time-consuming, and the seed production scale is not easy to control due to the difference in the survival rate of sowing and the purity of the parents in each year
2. CMS lines and restorer lines also need to be bred every year and provided for hybrid preparation. The annual breeding of the parents themselves is labor-intensive and time-consuming, which further increases the cost and expense of seed production
3. The parents will inevitably produce gene variation and degeneration during their own reproduction process, which will change the special combining ability of the hybrid combination, reduce the heterosis, and the yield and quality of hybrid cotton cannot be guaranteed. Generally, it will be popularized for about ten years. species are severely degraded and must be replaced by new hybrids
The utilization time of heterosis is short, the benefit of hybrid breeding is low, and the cost of hybrid cotton production is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The first embodiment of the present invention is a method for insect pollination and seed production of a cotton nuclear male sterile dual-purpose line under perennial root conditions, the steps of which are as follows:

[0022] A. In the first year, in the seed production area where cotton plants can survive the winter, that is, the area with the lowest winter temperature ≥ 10°C, plant the insect-resistant cotton male sterile line as the female parent and the restorer line as the male parent. Pull out 50% of the fertile plants in the female parent; at the flowering and boll stage, use insects for pollination, after the pollination, all the male parents are pulled out, and the hybrid seeds are harvested after the cotton bolls of the female parent are mature for large-scale production.

[0023] B. After the harvest of the female parent is finished, one row of plants in every 3-5 rows is pulled out, and the remaining plants survive the winter naturally.

[0024] C, prunin...

Embodiment 2

[0032] The way of this example is basically the same as embodiment one, and the difference is only: in the first year flowering and boll setting stage in A step, artificial pollination is carried out, rather than insect pollination.

[0033] In this embodiment, artificial pollination is carried out at the flowering and bolling stage of the first year of planting without insect pollination, so that pesticides can be applied at the flowering and bolling stage to thoroughly avoid damage to cotton plants by diseases and insect pests; so that the cotton plants of the first year The growth and development are more robust, laying a good foundation for the growth of the perennial cotton in the following years, so that the cotton grown from the perennial roots in the second year and subsequent years is well developed, and the hybrids have high yield and good quality.

[0034] When the present invention is implemented, only five steps of A-E can be carried out, that is, seeds are harvest...

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PUM

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Abstract

The invention provides an insect pollination seed producing method for the cotton nuclear sterile male lines in the rhizocarpous condition, the steps of the invention are that: in the region where the cotton is able to live through the winter, the cotton worm-resistant nuclear sterile male lines are planted as a female parent and the restorer line are planted as a male parent; during the bud period of the first year, the viable plants in the female parent are pulled out, and in the flowering and boll setting period, the production of the crossbreed is performed by use of the artificial assistant pollination or insect pollination; in winter, the female parent is rhizocarpous, the male parent is sleeved and planted into the rhizocarpous cotton of the second year of which the female plant is 100 percent sterile plant; in the flowering period, the cotton crossbreed seeds by means of insects pollination are adopted. In the third year and later, the insect pollination is performed in the flowering period by utilizing the female parent and the male parent of the rhizocarpous cotton to produce the crossbreed seeds. The advantage of the crossbreed can be kept for a long time and the planting output and the quality can be assured, the method promises planting one year and gaining crossbreed seeds continuously for many years; and by utilizing the insect pollination, a large amount of labor forces are saved and the seed production cost is low, which is convenient for the commercialized application and has extremely important significance in the popularization of the utilization technology of the cotton crossbreed advantages.

Description

technical field [0001] The invention relates to a method for preparing hybrids of cotton. Background technique [0002] The research and utilization of cotton heterosis is at the leading level in the world, and among them, nuclear male sterility is the most widely used. However, the existing GMS two-line method and the GMS secondary method for seed production all need to use artificial pollination, which requires a large amount of labor and high costs. The workload of artificial pollination is large, which limits the production of cotton hybrid seeds and the promotion and utilization of hybrids. This is a difficult problem that needs to be overcome. In addition, there are the following problems in the hybrid seed production of the two-line male sterile method: 1. When preparing hybrids every year, it is necessary to sow the male sterile line and the restorer line parents, and it is not possible to sow seeds for multiple harvests at one time. To remove sterile plants and mi...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 张小军牟方生张相琼毛正轩岳福良张超张小红龚一耘李文君罗征平胡吉远
Owner 四川省农业科学院经济作物育种栽培研究所
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