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Method for preparing and identifying cotton crossbreed

A hybrid and cotton technology, applied in the application field of agricultural biotechnology, can solve the problems of reducing production level, difficult to distinguish, harming the interests of cotton farmers, etc., and achieves the effects of strong practicability, accurate identification and reduction of economic losses.

Inactive Publication Date: 2009-10-28
王留明 +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many quality problems of cotton hybrids in the market, mainly because the purity of hybrid cotton seeds is difficult to be effectively controlled
Because cotton plants have the habit of unlimited growth, they are subshrubs and broad-leaved in form, and it is difficult to distinguish between different varieties in the group state. 1 Substitute Blend F 2 Generations of counterfeiting behaviors occur from time to time, which not only directly damages the interests of the majority of cotton farmers, but also prevents the seed properties of hybrid cotton from being fully expressed in large-scale production, reduces its due production level, and affects the health of the hybrid cotton industry. develop

Method used

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  • Method for preparing and identifying cotton crossbreed
  • Method for preparing and identifying cotton crossbreed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Using the offspring material HB321 obtained from the distant hybridization of Gossypium hirsutum L. and Gossypium bickii with the safflower trait of HB (Hirsutum-Bickii) from Gossypium bickii as the donor of the HB safflower trait , using cotton varieties Lumianyan No. 16, No. 17, No. 22, No. 28, and insect-resistant cotton lines 5A58, 118, and 20R37 with high yield, high quality, disease resistance, and insect resistance as recurrent parents, and carried out reintroduction Cross-breeding genetic improvement, to obtain a new germplasm material with HB safflower traits, that is, HB safflower upland cotton pure line varieties.

[0020] The hybrid produced by crossing the HB safflower variety as the male parent and the conventional white-flowered upland cotton variety Lu 05H9 as the female parent, because the HB safflower trait is a single-gene dominant inheritance, F 1 The generation plants should show red flowers and purple-red base spots, so F can be quickly determined ...

Embodiment 2

[0022] Using the offspring material HB321 obtained from the distant hybridization of Gossypium hirsutum L. and Gossypium bickii with the safflower trait of HB (Hirsutum-Bickii) from Gossypium bickii as the donor of the HB safflower trait Cotton varieties Lumianyan No. 16, No. 17, No. 22, No. 28, and insect-resistant cotton lines 5A58, 118 and 20R37 with high yield, high quality, disease resistance and insect resistance are used as recurrent parents. Since 2000 In 2006, a new germplasm material with HB safflower traits was obtained, that is, a pure line of HB safflower upland cotton.

[0023] A hybrid produced by crossing HB safflower variety as female parent and white flower variety Lu HB Biaoza-1 as male parent, hybrid F 1 There are a large number of selfing bolls or mixed with safflower conventional species. Will F 1 F 2 Planting observation, the isolated white-flowered strain rate in the population is significantly lower than 25%; the F 1 The BC of random testcross betw...

Embodiment 3

[0025] Using the offspring material HB321 obtained from the distant hybridization of Gossypium hirsutum L. and Gossypium bickii with the safflower trait of HB (Hirsutum-Bickii) from Gossypium bickii as the donor of the HB safflower trait Cotton varieties Lumianyan No. 16, No. 17, No. 22, No. 28, and insect-resistant cotton lines 5A58, 118 and 20R37 with high yield, high quality, disease resistance and insect resistance are used as recurrent parents. Since 2000 In 2006, a new germplasm material with HB safflower traits was obtained, that is, a pure line of HB safflower upland cotton.

[0026] Using HB safflower pure line conventional variety HB118 as safflower to mark F 1 generation hybrids. Will F 1F of self-crossing or random testcrossing with conventional white cotton varieties (female and male parents can be used) 2 and BC 1 f 1 Planting observation, the plant is full of safflower, and the normal safflower is marked F 1 F 2 BC with 3:1 segregation of safflower and wh...

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Abstract

The invention relates to a method for producing a cotton crossbreed, which uses a pure line variety of an HB red flower upland cotton as a male parent or a female parent to perform hybridization with a normal white flower parent material so as to generate a crossbreed and belongs to the field of agricultural biotechnology. The red flower upland cotton has stable characteristic heredity, and the expression of the red flower upland cotton is not influenced by climate, cultivation conditions and the like; through field planting observations of F1, F2 or BC1F1, the purity and the truth of an F1 generation crossbreed intuitively, quickly and easily according to flower color separation, and the identification result is reliable, quick and accurate. A false crossbreed can be pulled out during the bud stage in production to reduce the economic loss caused by problems of crossbreed purity, and the method provides an effective technical support for the protection of a cotton new variety and the seed quality detection control for cotton seed enterprises and majority of cotton farmers, and has important application value and social meaning.

Description

technical field [0001] The invention belongs to the application field of agricultural biotechnology, and relates to a method for producing a hybrid by using a pure HB safflower upland cotton variety as a male parent or a female parent, and a normal white-flowered parent material, and identifying the purity and authenticity of the seeds Methods. Background technique [0002] Cotton is an important economic crop in my country, which is of great significance to the safety of raw material supply and agricultural development of my country's textile industry. Like most crops, cotton F 1 The first generation of hybrids also has obvious heterosis, and the development of hybrid cotton has become an important technical approach to further improve cotton yield and benefit. Since the 1990s, the planting area of ​​hybrid cotton mainly in my country, India and other cotton-producing countries has been expanding. In the cotton area of ​​the Yangtze River Basin in my country, the coverage...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H5/02
Inventor 王留明王家宝赵军胜陈莹高明伟杨静王秀丽
Owner 王留明
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