The method and application of detection of single nucleotide polymorphism of sheep pcnp gene by pcr-rflp
A single nucleotide polymorphism, sheep technology, applied in the field of molecular genetics, can solve problems such as lack of research
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[0033] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are intended to explain rather than limit the present invention.
[0034] The present invention uses PCNP gene conserved sequences to design primers to amplify the 686bp fragment of the second exon of PCNP gene, uses Hu sheep and small-tailed Han sheep genomes as templates, and conducts PCR amplification, and the amplified product is sequenced to find the single-point sequence of the amplified fragment. Nucleotide polymorphisms; carry out trait association analysis for discovered single nucleotide polymorphisms and provide detection methods, making the nucleotide polymorphism of PCNP gene a molecular genetic marker that can be quickly and easily detected, To provide a basis for accelerating the establishment of sheep populations with high-quality economic traits such as Hu sheep and Small-tailed Han sheep.
[0035] a. Detecti...
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