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Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof

A kind of technology of single nucleotide polymorphism, detection method

Inactive Publication Date: 2010-05-12
NORTHWEST A & F UNIV
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Problems solved by technology

Due to the lack of research in the field of genetic variation of the SCD gene in Chinese dairy goats, the research on the function of the gene locus and the relationship between the genetic variation of the gene and economic traits (such as: lactation, growth and development, etc.) have become blank

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  • Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof
  • Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof
  • Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof

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Embodiment Construction

[0034]The invention uses the conservative sequence of the SCD gene to design primers to amplify the 5th exon and the 362bp fragment of the flanking region of the SCD gene, respectively use the genomes of two dairy goat varieties as templates to perform PCR amplification, and purify the PCR products. After sequencing Find the single nucleotide polymorphism of the amplified fragment; perform a trait correlation analysis on the found single nucleotide polymorphism, and provide its detection method, making the nucleotide polymorphism of the SCD gene a fast, Molecular genetic markers for convenient detection provide a basis for accelerating the establishment of dairy goat populations with high-quality economic traits.

[0035] a. Cloning of partial DNA sequence of dairy goat SCD gene and detection of its polymorphism

[0036] 1. Collection and processing of dairy goat blood samples

[0037] Take 10 mL of milk goat blood sample, add 500 μL of 0.5 mol / L EDTA for anticoagulation, slo...

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Abstract

The invention discloses single nucleotide polymorphism of SCD genes in dairy goat and a detection method thereof. The single nucleotide polymorphism of the genes comprises G / A single nucleotide polymorphism which is the 5001st in the SCD genes in the dairy goat. The detection method comprises the following steps: taking the DNA of the whole genome of the dairy goat to be detected containing the SCD genes as a template and a primer pair P as a primer to carry out PCR amplification on the SCD genes in the dairy goat; after using restriction enzyme NdeI to digest the PCR amplified product, carrying out agarose gel electrophoresis on amplified fragments after restriction enzyme; and identifying the 5001bp base polymorphism of the SCD genes in the dairy goat according to the electrophoresis results. The method screens and detects the molecular genetic markers closely related to the milk production traits of the dairy goat on the DNA level to be used for assisted selection and molecular breeding of the dairy goat and speed up find breeding of the dairy goat.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a single nucleotide polymorphism (SNP) of a functional gene of a dairy goat as a molecular genetic marker, in particular to a single nucleotide polymorphism of the SCD gene of a dairy goat and its detection method. Background technique [0002] Dairy goat is one of the main animal husbandry in my country, but now it is facing the pressure of insufficient provenance of excellent dairy goat breeds and poor genetic quality of the population. Traditional breeding methods apply the phenotypes of test animals and the genetic information of their parents, grandparents, and other relatives in small animal models, assuming that traits are affected by genetic differences in many genes, each of which contributes relatively little to the trait , so there is inevitably some bias in the estimation of traits. Molecular genetic markers are one of the fastest-growing fields in modern genetics in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏陈忠琦蓝贤勇李转见屈玉娇刘艳丽雷初朝
Owner NORTHWEST A & F UNIV
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