A Molecular Marker Method for Selecting Growth Traits of Goats Using Nucleolin Gene

Abstract

The invention discloses a molecular marking method selecting goat growth traits using nucleophosmin gene. According to the method, a goat gene group DNA sequence is used as a template, under the condition of existence of Taq DNA polymerase, buffer environment, Mg <2+>, and dNTPs, four pairs of primer are used for amplifying a Nucleophosmin (NPM) gene 5'UTR region and exon 1 under the condition of PCR condition, and the size of a target segment of an amplified product is determined according to agarose gel electrophoresis; an amplified site of primer P3 is found lacking of 18bp basic group through detection of PCR amplification products of primers P1-P4 by use of polyacrylamide gel electrophoresis, then genotyping and gene frequency analysis are performed on the amplification product of the primer P3, and associated analysis on the amplification production of the primer P3 and growth traits of a milk goat and a Boer goat in the central Shaanxi plain is performed. Results indicate that the SNPs site of the Nucleophosmin gene exon 1 detected by the primer P3 can be used as a molecular mark for goat growth trait selection.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and specifically relates to a molecular marker method for selecting growth traits of goats by using the nucleophosmin gene. The results showed that the SNPs detected by primer P3 in Exon 1 of Nucleophosmin gene can be used as molecular markers for the selection of growth traits in goats. Background technique [0002] With the rapid development of molecular biology technology, especially the advent of Polymerase Chain Reaction (Polymerase Chain Reaction) technology and the improvement of electrophoresis technology, various molecular genetic marker technologies have emerged. The cultivation of provides new ways and methods. In particular, the combination of molecular marker technology and conventional breeding technology has given birth to a brand-new selection method, that is, marker-assisted selection (Marker assistant selection). It can overcome the environmental deviation and sampling error ...

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Problems solved by technology

Among these SNP detection techniques, DNA sequence determination is the most accurate SNP detection method, but its detection cost is extremely expensive, and large-scale instruments such as DNA sequencers are required. At the same time, very skilled technicians and Experience, so DNA sequence determination is not an ideal SNP detection method applied in actual production; of course, the combination of PCR-SSCP and DNA sequencing can be used to detect SNPs can appropriately reduce the detection cost, but the experimental process of PCR-SSCP is relatively long, The operation is cumbersome, and there are false negative problems in the experimental process, so it is not an ideal SNP detection method; AS-PCR method, as a new type of SNP detection method, has very broad prospects in the future application field. Special primers need to be designed, and can only target specific gene loci. At the same time, there is a probability of false detection in the detection process. Detection of SNP sites requires detection platforms such as plate reader, gene chip, microsphere array technology and mass spectrometer, which is not very practical for general molecular laboratories

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