A Method for Individual Breeding Value Assessment of Random Populations of Turbot

A technology of breeding value and turbot, applied in the field of genetic breeding of seawater animals, can solve problems such as limited reconstruction, unclear genetic background, difficult breeding, etc., achieve accurate estimation of breeding value, accurate genetic relationship, and accelerate the process of breeding Effect

Active Publication Date: 2017-02-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the long sexual maturity cycle of turbot (2-3 years), most breeding farms currently have a limited number of broodstock, let alone complete physical pedigree records; at the same time, turbot is an introduced species, and the genetic background of the population It is not clear, which also caused great difficulties for the selection and breeding of improved turbot species
Although pedigree reconstruction can be realized by using molecular markers, this kind of reconstruction is very limited. For example, it can only be reconstructed to the parental generation, and only the genotype combination of the parents can be calculated, but the specific paternal and maternal gene types cannot be calculated. genotype

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  • A Method for Individual Breeding Value Assessment of Random Populations of Turbot
  • A Method for Individual Breeding Value Assessment of Random Populations of Turbot
  • A Method for Individual Breeding Value Assessment of Random Populations of Turbot

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Embodiment 1

[0027] 1) Construction of turbot family

[0028] At the beginning of 2013 (January), the reproductive regulation of candidate turbot broodstock was carried out by controlling temperature and light. When the water temperature is suitable at the end of April, select broodstock candidates with mature gonads, complete body shape, normal body color, active feeding, healthy and lively, no disabilities, and weighing more than 2 kg for artificial insemination to build a turbot family. Specifically: the fertilized eggs are placed in an 80×60×60cm hatching net cage for inflatable hatching, and the seawater temperature is maintained at 14-15°C. After 3 days of hatching, absorb the floating eggs and follow the 20ml / m 2 (350 grains / ml) standard, moved from the hatching pond into the circular glass fiber reinforced plastic cylinder (0.5m 2 , volume 0.5m 3 ) was slightly aerated, hatched in still water until emergence, and each family was cultivated separately; according to the convention...

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Abstract

The invention aims to provide a method for evaluating the individual breeding value of a turbot random population, so as to calculate an individual parentage coefficient under the condition of non-existence of physical pedigree, and further carry out genetic parameter evaluation. Pedigree recording is not needed in the method, but in traditional breeding, physical marking needs to be performed on a breeding population, and pedigree is recorded to evaluate an inter-individual genetic relationship, so that the workload is high. According to the method, the inter-individual genetic relationship can be directly evaluated by utilizing molecules, so as to eliminate the step of pedigree recording. Moreover, the inter-individual genetic relationship can be evaluated more accurately according to the method.

Description

technical field [0001] The invention belongs to the technical field of seawater animal genetics and breeding, and in particular relates to a method for evaluating the individual breeding value of turbot random populations. Background technique [0002] Turbot (Scophthalmus maximus L.) is a bottom-dwelling marine fish native to Europe. It has the advantages of rapid growth, low temperature resistance, unique flavor, docile personality, and is suitable for intensive farming. It is an excellent marine aquaculture in Europe and other places. One of the varieties. Since the breakthrough of productive nursery technology in 1999, the turbot breeding industry has developed rapidly in my country and has become an important fish breeding species in the northern coast of my country. Since my country is not the origin of turbot, the medium- and long-term aquaculture production depends on the country of origin of European turbot to provide and supplement provenance. At the same time, d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 王伟继吕丁胡玉龙栾生孔杰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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