The present application relates to the technical field of
molecular biology, and discloses a 74-core
molecular marker set for
cold tolerance identification of
Pennisetum, a primer group, a detection
reagent, a kit, a
gene chip and applications thereof.The present application collects core
germplasm resources of
Pennisetum systematically, and constructs a GWAS
population by using multi-environment (different altitudes) conditions, fully covers extensive genetic and phenotypic variations, and lays a foundation of representative materials for
genetic analysis of
cold tolerance.In the aspect of
phenotype evaluation, the present application innovatively proposes a method of "single root double stem node oblique planting" combined with the determination of the number of root
cutting seedlings after natural
overwintering, establishes a scientific, stable and standardizable
evaluation system for
cold tolerance and regeneration, and significantly improves the reliability and efficiency of
phenotype identification.The present application is not affected by human and climate environment, realizes efficient and accurate early cold tolerance identification of
Pennisetum, greatly improves the efficiency and scale of breeding screening, and accelerates the breeding process.