10 results about "Kit gene" patented technology

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention relates to the technical field of biomedical detection, in particular to a urinary tract epithelium cancer detection marker composition based on ONECUT2 and DMRTA2 gene methylation, which comprises a CpG island region of an ONECUT2 gene, a CpG island region of a DMRTA2 gene and a kit for detecting urinary tract epithelium cancer. The CpG island region of the ONECUT2 gene is located in chr18: 57441548-57441674, and the CpG island region of the ONECUT2 gene is located in The CpG island region of the DMRTA2 gene is located in chr1: 50418714-50418823, and the CpG island region of the DMRTA2 gene is located in chr1: The kit for detecting urothelial carcinoma comprises a first primer pair and a first probe which are used for specifically detecting the methylation state of the CpG island region of the ONECUT2 gene in the claim 1, and a second primer pair and a second probe which are used for specifically detecting the methylation state of the CpG island region of the DMRTA2 gene. The urinary tract epithelium cancer detection marker composition based on ONECUT2 and DMRTA2 gene methylation has high sensitivity and high specificity, the sensitivity and the specificity are both 90% or above, early-stage and low-grade urinary tract epithelium cancer can be effectively detected, comprehensive coverage is achieved, and the urinary tract epithelium cancer detection marker composition is not only suitable for bladder cancer, but also suitable for upper urinary tract epithelium cancer such as renal pelvis cancer and ureter cancer.

The invention belongs to the technical field of biology, and particularly relates to a CFTSR gene, a CFTSR gene simulant and application of the CFTSR gene simulant. The nucleic acid sequence of the CFTSR gene is as shown in SEO ID NO. 1. The nucleic acid sequence of the CFTSR gene simulant is as shown in SEO ID NO. 2. The CFTSR gene provided by the invention can be used as a marker for fibroblast activation, and is used for a kit for detecting cardiac fibroblast activation. The CFTSR gene simulant can be used as a therapeutic drug for myocardial infarction injury, and the overexpression of the CFTSR gene can significantly inhibit the proliferation of fibroblasts and activate the repair of damaged myocardial tissues. Based on CFTSR mimics, a simulant of the CFTSR gene is constructed, and the simulant can be conveyed to the position of a heart lesion and can be used as a medicine for treating myocardial infarction injury, so that effective treatment of the lesion is realized.

The application provides a method for breeding a pure white color Wuzhishan pig. The method adopts a strategy of phenotype breeding as the main part and molecular marker monitoring as the auxiliary part, simultaneously uses KIT gene detection to confirm the genotype background of each generation, uses SSR genetic diversity monitoring to guide scientific mating and external blood introduction, avoids inbreeding recession, and through breeding, a new strain of Wuzhishan pig with pure white color, stable heredity and rich blood is obtained.

The invention provides a c-kit gene and application of an overexpression vector of the c-kit gene in preparation of drugs for preventing and treating development retardation / morphological deformity of odontogenic organs including enamel. The nucleotide sequence of the c-kit gene is shown as SEQ ID No.1. Compared with the prior art, the application has the following advantages: the application provides the c-kit gene and the application of the overexpression vector of the c-kit gene in the preparation of the medicine for preventing and treating the development retardation / morphological deformity of the odontogenic organ, and records that the c-Kit-PI3K-Creb3l2 signal axis which is not recognized previously is crucial to the development of normal teeth; the pathway maintains the progenitor cell characteristics and proliferation ability of the dental epithelial cells, and ensures normal growth and mode; the technique promotes the understanding of the tooth formation signal network and contributes to a wider field of regenerative dentistry, where manipulation of key signal pathways such as c-Kit and PI3K may provide information for tooth bioengineering or repair strategies.

The present invention provides a sgRNA that specifically recognizes the porcine KIT gene, its encoding DNA, a kit, and applications, relating to the field of genetic engineering technology. The nucleotide sequence responsible for recognizing the target fragment region in the sgRNA is the sequence shown in SEQ ID NO. 1, SEQ ID NO. 2, or SEQ ID NO. 3. This sgRNA enables the CRISPR / Cas9 gene editing system to delete redundant mutant copies of the KIT gene in the porcine genome, allowing the KIT gene to normally express the biologically active KIT protein, thereby changing the pig's coat color.

The invention relates to a molecular marker primer related to duck black matter characters and application of the molecular marker primer, and belongs to the technical field of biology. The KIT gene SNP molecular marker is located at the 51648174 basic group of chromosome 4 of a duck reference genome GCF047663525.1 version, the basic group is mutated into T or C, and the genotypes are T / T, T / C and C / C. The ducks of the C / C and T / C genotype individuals show a ubiquitous phenotype, and the ducks of the T / T genotype individuals show a non-ubiquitous phenotype. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing and genetic typing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used for efficiently and accurately identifying the duck black matter character genotype, the accuracy and efficiency of duck black matter character identification are improved, and the molecular marker has important value for protection and utilization of excellent germplasm resources of black-bone ducks.

Provided herein are pharmaceutical compositions comprising antibodies that immunospecifically bind to KIT, a receptor tyrosine kinase, and uses thereof. Also provided are kits and methods for producing such pharmaceutical compositions. KIT (or c-Kit) is a type III receptor tyrosine kinase encoded by the c-kit gene. KIT comprises five extracellular immunoglobulin (ig)-like domains, a single transmembrane region, an inhibitory cytoplasmic juxtamembrane domain, and a split cytoplasmic kinase domain separated by a kinase insert segment.

The present application relates to the technical field of molecular biology, and discloses a 74-core molecular marker set for cold tolerance identification of Pennisetum, a primer group, a detection reagent, a kit, a gene chip and applications thereof.The present application collects core germplasm resources of Pennisetum systematically, and constructs a GWAS population by using multi-environment (different altitudes) conditions, fully covers extensive genetic and phenotypic variations, and lays a foundation of representative materials for genetic analysis of cold tolerance.In the aspect of phenotype evaluation, the present application innovatively proposes a method of "single root double stem node oblique planting" combined with the determination of the number of root cutting seedlings after natural overwintering, establishes a scientific, stable and standardizable evaluation system for cold tolerance and regeneration, and significantly improves the reliability and efficiency of phenotype identification.The present application is not affected by human and climate environment, realizes efficient and accurate early cold tolerance identification of Pennisetum, greatly improves the efficiency and scale of breeding screening, and accelerates the breeding process.

The application provides application of a c-kit gene and an overexpression vector thereof in preparation of a medicine for preventing and treating tooth-derived organs, including enamel development retardation / morphological deformity, and a nucleotide sequence of the c-kit gene is shown as SEQ ID No. 1. Compared with the prior art, the application has the following advantages: the application provides application of the c-kit gene and the overexpression vector thereof in preparation of the medicine for preventing and treating tooth-derived organ development retardation / morphological deformity, and a previously unidentified c-Kit-PI3K-Creb3l2 signal axis is recorded, which is crucial for normal tooth development; the path maintains the progenitor cell characteristics and proliferation capacity of dental epithelial cells, and ensures normal growth and pattern; the technology promotes the understanding of a tooth formation signal network, and is helpful for a wider field of regenerative dentistry, wherein manipulation of key signal pathways such as c-Kit and PI3K can provide information for tooth bioengineering or repair strategies.