Method for breeding high-temperature-resistant Paralichthys olivaceus new varieties
A high-temperature-resistant brown flounder technology is applied in the field of breeding of new high-temperature-resistant brown flounder varieties, which can solve the problems of short breeding cycle, growth, feeding and other physiological and ecological behavior effects, so as to avoid inbreeding and accelerate Breeding progress, effect of clear genetic background
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[0006] The implementation of the present invention operates according to the following steps:
[0007] 1. Select 200 large, healthy and disease-free 4-5-year-old wild domesticated flounder as backup parents, and carry out PIT electronic labeling; after labeling, intensive culture is carried out to promote gonad maturation;
[0008] 2. For broodstock with good selective gland maturity and healthy individuals, use artificial insemination to establish 50 full-sib families of flounder, including 20 maternal and half-sib families. Before the identification of the F1 generation of dye markers, separate the breeding of different families, and the breeding environment should be kept consistent;
[0009] 3. Mark the fry of each family with a total length of about 10cm, and distinguish the families by the injection site of the dye and the color of the dye. Take 50 fish from each family and breed them in the same pond until they are 4 months old. ;
[0010] 4. Heat the polyculture tank...
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