Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection primer, detection kit, detection method and application of chicken Columbia feather genotype

A detection kit and detection primer technology are applied in the field of animal genetics and breeding, which can solve the problem of lack of a better detection method for chickens, and achieve the effects of a fast molecular breeding method, rapid detection and accurate results.

Active Publication Date: 2020-10-30
HENAN AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chicken feather color is a quality trait, which is controlled by many genes, and its inheritance is also a complicated process. At present, there is no good method for detecting the genotype of chicken Columbia plumage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection primer, detection kit, detection method and application of chicken Columbia feather genotype
  • Detection primer, detection kit, detection method and application of chicken Columbia feather genotype
  • Detection primer, detection kit, detection method and application of chicken Columbia feather genotype

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The nucleotide sequence of the detection primer of the chicken Columbia feather genotype in the present embodiment is as follows:

[0041] Upstream primer P-F: 5'-CGTATACACAGCCTGGCCTAA-3';

[0042] Downstream primer P-R: 5'-ATTCCTGTGTGTGGTGGAGTG-3'.

[0043] Example 1 of the Detection Kit for Chicken Columbia Feather Genotype

[0044] In this embodiment, the detection kit for chicken Columbia feather genotype includes: detection primers, 2×Taq PCRmasterMix, ddH 2 O. Among them, 2×Taq PCR masterMix is ​​provided by Kangwei Century Biotechnology Co., Ltd., product number Lot01036 / 30146.

[0045] The nucleotide sequences of the detection primers are as follows:

[0046]Upstream primer P-F: 5'-CGTATACACAGCCTGGCCTAA-3';

[0047] Downstream primer P-R: 5'-ATTCCTGTGTGTGGTGGAGTG-3'.

[0048] Example 1 of the Detection Method of Chicken Columbia Feather Genotype

[0049] The detection method of chicken Columbia plumage genotype in the present embodiment may further compri...

Embodiment 2

[0061] The technical flow diagram of the detection method of the chicken Columbia plumage genotype of the present embodiment is as follows figure 1 As shown, it mainly includes the following steps:

[0062] 1) Preparation and storage of samples

[0063] 15 Jute Yu Gushi roosters and 150 H-series hens of Columbia Yuyufen No. figure 2 shown), including 39 F1 Colombian cocks and 38 jute hens; F2 chickens are the offspring of 2 F1 Colombian cocks and 20 F1 F1 jute hens, including 181 Columbian cocks 212 Colombian hens, 192 jute roosters and 186 jute hens; the F3 generation A group selected F2 Colombian roosters and Columbian hens to cross, and produced 36 Columbian roosters and Columbian hens 18 chickens and 22 hens with jute feathers; Group B selected F2 Colombian cocks and hens with jute feathers to cross, and produced 15 Columbia cocks, 16 Colombian hens, 15 jute cocks and 14 jute hens Only. Take the isolated tissues, organs or blood of the chicken to be tested, extract th...

Embodiment 3

[0086] The detection method of the chicken Columbia feather genotype of the present embodiment mainly comprises the following steps:

[0087] 1) Preparation and storage of samples

[0088] 96 Gushi Chickens (Jute Feather), 96 Lushi Chickens (Jute Feather), 96 Changshun Chicken Green Shell Layers (Yellow Feather), 96 F2 resource groups of Gushi Chicken and Anka Chicken (GxA F2, yellow feather), 96 recessive white-feather chickens (white feather), 96 Xichuan silky chickens (white feather), 96 AA chickens (white feather), 96 Dongxiang chicken green-shell layer hens (black feather). Take the isolated tissues, organs or blood of the chicken to be tested, extract the DNA with the proteinase K method, and store the DNA samples in a 4°C refrigerator for later use.

[0089] Step 2), step 3) and step 4) are the same as Example 2 of the detection method of chicken Columbia feather genotype.

[0090] 5) Test results

[0091] Test results such as Figure 4 shown in . The typing result...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a detection primer, a detection kit, a detection method and application of a chicken Columbia feather genotype, and belongs to the technical field of animal genetic breeding.According to the invention, the insertion of 63bp base sequences from the 33rd site to the 95th site in the upstream region sequence of the RAI14 gene is found to be closely related to the Columbia feather character; therefore, a PCR (Polymerase Chain Reaction) genotyping method aiming at the insertion is established by utilizing the characteristics of the upstream region of the RAI14 gene, so that the Columbia feather chicken gene can be typed. The method for detecting the chicken Columbia feather genotype is simple to operate, rapid in detection and accurate in result, is beneficial to moreeffectively and rapidly selecting the Columbia feather character in breeding practice, accelerates the breeding progress, saves the breeding cost, and provides a rapid molecular breeding method for breeding the Columbia feather chicken character.

Description

technical field [0001] The invention relates to a detection primer, a detection kit, a detection method and an application of chicken Columbia feather genotype, and belongs to the technical field of animal genetics and breeding. Background technique [0002] The feather color of chickens is very rich, which is one of the important breed characteristics of chickens, and is also an important selection index in chicken breeding work. The shallow reed feathers of chickens are also called Colombian feathers. The specific manifestations are: the main body of the feathers is basically white feathers, and the ends of the feathers on the neck, wings and tail have black horizontal spots in the shape of horizontal stripes and shallow reeds. It is sex-linked Colombian feathers. Sex-linked Columbia plumage has gained more attention and research because of its distinctive characteristics and sex-linked inheritance. [0003] As a trait, Colombian feathers are shown in the Yufen 1 H line, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 康相涛李转见田亚东刘丹丽韩瑞丽孙桂荣王鑫磊王永才李国喜闫峰宾蒋瑞瑞李红李文婷刘小军王彦彬
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com