Method for improving aquatic animal whole-genome selective breeding efficiency

Abstract

The invention discloses a whole-genome selective breeding method suitable for aquatic animals. Specifically, high-density SNP typing is carried out on individuals of a breeding basic group or a core group; the phenotypic value of the target character is determined; whole-genome association analysis (GWAS) is carried out by utilizing the SNP typing data and the phenotypic data to obtain a significance P value of each SNP marker; the marks are sorted from low to high according to P values; according to different traits, different marker number combinations sorted in the top are selected according to the P values, analysis is performed by adopting methods such as GBLUP, Bayes B and the like, the prediction accuracy of the different marker numbers selected according to the P values is evaluated through cross validation, and finally, the marker combination with the highest prediction accuracy is determined. SNP typing is conducted on the candidate population or the next-generation breedingpopulation through the screened optimal marker combination, breeding value prediction is conducted on the candidate population or the next-generation breeding population through adoption of GBLUP or BayesB or ssGBLUP or other methods, and therefore, the prediction accuracy can be remarkably improved.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and in particular relates to a whole-genome selective breeding method suitable for aquatic animals. Background technique [0002] Genome-wide selective breeding was first proposed by Meuwissen in 2001, which uses SNP markers covering the whole genome to type reference populations and candidate populations, and obtains the effect of each SNP marker based on the phenotype and genotype information of the reference population. SNP effects are predicted and evaluated for candidate populations. With the development of high-throughput SNP typing technology, this method has been applied to more important economic animals including dairy cows, pigs, and chickens. [0003] In order to improve the efficiency of genome-wide selection, researchers have carried out a series of work. On the one hand, they are developing high-density SNP typing methods. Currently, the commonly used SNP chip generally has about...

AI Technical Summary

Problems solved by technology

[0004] However, for aquatic animals, the value of a single individual is often low, and the application of genome-wide selection methods is still greatly limited, mainly because the relatively high cost of SNP typing limits the application of this method. In addition, the improvement of breeding efficiency is not enough. This obviously limits the wide application of this method

Images