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SNP molecular marker located on pig chromosome 9 and related to healthy piglet number and healthy piglet rate and application of SNP molecular marker

A technology of molecular marker and number of healthy piglets, applied in the field of molecular biotechnology and molecular markers, to achieve the effect of increasing the number of healthy children and the rate of healthy children, increasing core competitiveness and increasing frequency

Active Publication Date: 2022-02-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, it is necessary to clearly realize that due to the constraints of genetic factors, breeding technology, production management, etc., there is still a large gap between the reproductive performance of sows in my country and developed countries (such as Denmark), and there is still a long way to go in improving the reproductive performance of sows. Larger breeding space

Method used

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  • SNP molecular marker located on pig chromosome 9 and related to healthy piglet number and healthy piglet rate and application of SNP molecular marker
  • SNP molecular marker located on pig chromosome 9 and related to healthy piglet number and healthy piglet rate and application of SNP molecular marker
  • SNP molecular marker located on pig chromosome 9 and related to healthy piglet number and healthy piglet rate and application of SNP molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Experimental animals

[0030] The experimental pig group used in the present invention is 536 purebred Duroc pigs from the Breeding Pig Branch of Wen's Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.

[0031] In this experiment, the Duroc pigs in this resource group were selected, and the pigs had free access to food and water, and the entire feeding method and feeding conditions were always consistent, which was a conventional method.

[0032] (2) Sample collection

[0033] The above-mentioned tail or ear tissues of piglets were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0034] (3) 50K SNP typing in the whole pig genome

[0035] Ear tissue or tail tissue was collected from each of the 536 Duroc pigs in the above-mentioned resource group, and the whole genome DNA was extracted by the standard phenol-chloroform method, and the DNA content of each sample was ac...

Embodiment 2

[0045] Example 2 Target DNA sequence amplification and sequencing

[0046] (1) Primer design

[0047] The DNA sequence of SEQ ID NO: 1 on pig chromosome 9 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html), and primers were designed using the primer design software Oligo 7. The DNA sequences of the designed primers are as follows:

[0048] P001-F: 5'-TCCATATTCTCACAGACACCAGGT-3',

[0049] P002-R: 5'-TGTTGGGCAGTCTTAGGAATGACT-3';

[0050] (2) PCR amplification

[0051] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 57.6°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0052] (3) DNA sequence determination

[0053] DNA sequence sequencing identification: carried out ...

Embodiment 3

[0057] Example 3 SNP site g.141C>T effect analysis of molecular markers

[0058] According to Table 1, compared with the dominant allele type CC of the SNP site g.141C>T, compared with the inferior allele TT type, it can increase 1.71 healthy calves and improve the healthy calves rate by 13.28%. Therefore, through molecular marker-assisted selection or genomic selection, gradually selecting and retaining CC-type pigs in the population can significantly increase the frequency of the dominant allele C allele, which is beneficial to the healthy piglet size and health of sows. The improvement of litter rate can speed up the breeding and improvement process of pigs, and finally effectively improve the economic benefits of pig breeding.

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Abstract

The invention provides an SNP molecular marker located on pig chromosome 9 and related to the healthy piglet number and the healthy piglet rate. The site of the SNP molecular marker corresponds to C > T mutation at the 33597148 site on a chromosome 9 of a reference sequence of an international pig genome version 11.1. By optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the number and rate of healthy piglets of core group sows are improved, and the improvement progress of related reproductive traits of the sows is accelerated, so that the economic benefit of breeding of boars is effectively improved.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and specifically relates to a SNP molecular marker located on chromosome 9 of pigs and related to the number of healthy piglets and the healthy piglet rate and its application. Background technique [0002] In modern pig production, reproductive traits of sows, as an important economic trait, are the key factors affecting the economic benefits of pig farms. Thanks to the continuous improvement of the reproductive performance of sows, my country's pig industry has become a large pig-raising country with the largest number of pigs in the world. The reproductive performance of sows has been significantly improved and great genetic progress has been made through the introduction of foreign excellent Duroc varieties and breeding and selection. But at the same time, it is necessary to clearly realize that due to the constraints of genetic factors, breeding techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 吴珍芳杨杰吴杰丁荣荣李紫聪郑恩琴蔡更元顾婷徐铮洪林君杨化强黄思秀
Owner SOUTH CHINA AGRI UNIV
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