Preparation method of high-efficient decay-promoting agent

A decay-promoting agent and high-efficiency technology, which is applied in the field of preparation of high-efficiency decay-promoting agents, can solve the problems of slow degradation of straw, low purity of strains, low activity of strains, etc., and achieve accelerated decomposition speed, high activity, and shortened cycle Effect

Inactive Publication Date: 2017-05-24
云南博隆生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is disclosed that the preparation of various bacterial strains is prepared by inoculating the bacterial strains into the prepared respective liquid medium for cultivation, the bacterial strains cultivated in these mediums are not highly active, and the preparation method is to directly inoculate the various bacterial strains The first-level culture seed liquid and the sub-inoculation liquid culture were mixed in proportion and carried out shaker

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0025] Experimental materials - preparation of culture medium:

[0026] Nutrient Agar medium: Pepton 5 g, Beef extract 30 g, NaCl 5g, Agar 15 g, add dH 2O to 1000 mL, pH: 7.0-7.2;

[0027] LB medium: Tryptone 10 g, Yeast extract 5 g, NaCl 10 g, add dH 2 O to 1000 mL, pH=7.0;

[0028] MS agar medium: 20 g of agar, 20 g of mannitol, 20 g of soybean powder, dilute to 1000 mL with deionized water, adjust the pH to 7.2 with 5 M NaOH, and sterilize twice under high temperature and high pressure;

[0029] PDA medium: 200g potato, add 1000ml water, boil for half an hour, filter with gauze, add 10-20g glucose and 17-20g agar, sterilize by autoclaving (121°C) for 20 minutes.

Embodiment 1

[0031] (1) Activation and purification of strains and expanded culture on a liquid shaker:

[0032] (1) Inoculate the fungi composed of Saccharomyces cerevisiae, Trichoderma viride, Aspergillus oryzae, and Rhizopus oryzae at a ratio of 25:15:25:15 to PDA medium, place them in a constant temperature incubator at 28°C, and cultivate Until the mycelium is abundant or spores are produced; pick the mycelia or spores of the fungus and inoculate them into the prepared fungal liquid medium for liquid shaker culture, the culture temperature is 28°C, and the shaker culture speed is 200 rpm;

[0033] (2) Inoculate actinomycetes composed of Streptomyces griseus and Streptomyces luteus at a ratio of 45:45 on MS agar medium for streak culture, place in a constant temperature incubator at 30°C, and cultivate to a single colony or spore production; pick single colonies or spores of actinomycetes and inoculate them into the prepared actinomycetes liquid medium for liquid shaker culture, the cu...

Embodiment 2

[0041] (1) Activation and purification of strains and expanded culture on a liquid shaker:

[0042] (1) Inoculate the fungi composed of Saccharomyces cerevisiae, Trichoderma viride, Aspergillus oryzae, and Rhizopus oryzae at a ratio of 30:20:30:20 on the PDA medium, place them in a constant temperature incubator at 28°C, and cultivate Until the mycelium is abundant or spores are produced; pick the mycelia or spores of the fungus and inoculate them into the prepared fungal liquid medium for liquid shaker culture, the culture temperature is 28°C, and the shaker culture speed is 200 rpm;

[0043] (2) Inoculate actinomycetes composed of Streptomyces griseus and Streptomyces luteus at a ratio of 50:50 on MS agar medium for streak culture, place in a constant temperature incubator at 30°C, and cultivate to a single colony or spore production; pick single colonies or spores of actinomycetes and inoculate them into the prepared actinomycetes liquid medium for liquid shaker culture, th...

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PUM

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Abstract

The invention discloses a preparation method of a high-efficient decay-promoting agent. The preparation method comprises the three steps of activation and purification of bacteria, enlarged culture of a liquid shaking table and preparation of solid bacterial powder, and comprises the concrete steps of inoculating fungi, actinomycetes and bacteria on respective culture media, and carrying out streak culture; picking single colonies of the bacteria and the actinomycetes or mycelia or spores of the fungi to inoculate into respective prepared liquid culture media, and culturing through the liquid shaking table; finally inoculating each bacterium of each liquid culture medium into a prepared culture material according to the proportion, dry-curing, mixing the dry-cured bacteria according to the proportion, and preparing the high-efficient decay-promoting agent with high fertility. The invention provides the preparation method of the high-efficient decay-promoting agent, which has the characteristics of capability of enabling the decomposing speed of straw or farmyard manures to be improved remarkably, high degradation ability, high decay promoting efficiency, and good effect.

Description

technical field [0001] The invention belongs to the technical field of corrosion accelerators, and in particular relates to a preparation method of high-efficiency corrosion accelerators. Background technique [0002] Using straw or farmyard manure to make organic fertilizer or directly composting and returning straw or farmyard manure to the field requires the addition of rot accelerators to make the raw materials decompose quickly. The process of straw or farmyard manure to promote decay mainly involves microorganisms and enzymatic degradation processes. The bacteria continuously decompose and transform straw and farmyard manure. The degradation products of straw or farmyard manure are rich in nutrients such as nitrogen, phosphorus and potassium, which can be used for crop growth. This means that the higher the activity of the beneficial bacteria in the rot accelerator, the faster it can promote the rot of straw and farmyard manure, and the better the effect of the rot acc...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20C05F3/00C05F11/00C05F17/00C12R1/645C12R1/885C12R1/69C12R1/845C12R1/125C12R1/25C12R1/225C12R1/12C12R1/07
CPCC05F3/00C05F11/00C05F17/00C12N1/14C12N1/20C05F11/08Y02W30/40Y02A40/20
Inventor 杨本寿李春代丽娟汪贵彬何兆禹
Owner 云南博隆生物科技开发有限公司
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