Method for preventing and treating verticillium wilt of cotton verticillium wilt seriously diseased fields

A technology for cotton verticillium wilt and verticillium wilt, applied in the field of agricultural biology, can solve problems such as difficult to reach plants, cotton field destruction, and ineffective effects, so as to reduce farmland pollution, improve soil structure and microbial flora, and reduce yield loss effect

Active Publication Date: 2019-05-07
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, crop rotation with non-host crops can effectively control soil-borne diseases, but at present, the phenomenon of land competition for grain and cotton in my country is serious, and it is difficult to implement crop rotation measures
Planting disease-resistant varieties is the most economical and effective measure to prevent and control soil-borne vascular diseases. However, at present, my country's cotton breeding for Verticillium wilt resistance has not made significant progress. Most varieties are disease-resistant varieties, and the disease will still occur in severe years of Verticillium wilt popular, inevitable
Spraying fungicides has little effect because it is difficult to reach the vascular part of the plant
Other measures, such as microbial preparations, inducers, etc., are environmentally friendly, but the control effect is not good, and it is difficult to effectively curb the occurrence and harm of Verticillium wilt
Especially for the fields where Verticillium wilt occurs seriously, such as Xinjiang cotton area, most of the cotton fields are old cotton fields that have been cultivated for many years, coupled with cultivation methods such as drip irrigation and straw returning to the field, resulting in serious occurrence of Verticillium wilt year after year, and some cotton fields even destroy species

Method used

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  • Method for preventing and treating verticillium wilt of cotton verticillium wilt seriously diseased fields
  • Method for preventing and treating verticillium wilt of cotton verticillium wilt seriously diseased fields

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of microbial fertilizer

[0034] Preparation of seed solution: activate the cotton endophytic fungus CEF08111 (Gibellulopsis nigrescens) on a PDA plate, pick a small amount of hyphae and inoculate it in Czapek liquid medium after 1 week, culture at 25°C, 150r / min, for 5 days, and divide Spore concentration greater than 1×10 7 Individual / mL, at this time the suspended spore liquid is the fermentation broth;

[0035] Preparation of microbial strains: Add 0.2Kg of boiling water to 1.0Kg of corn flour and mix well, then soak for 30min, add 0.3Kg of sand, mix well, put into polypropylene strain bags (15×55cm), each bag contains 0.5Kg, Seal with double sponge rings, autoclave for 30 minutes to obtain corn sand medium; after cooling to room temperature, the concentration of each bag is 1×10 7 15 mL of suspended spore liquid per mL, mixed evenly and placed in a culture room at 25°C for fermentation for 7 days, so that the yield of conidia reached 10 8 pcs / g. ...

Embodiment 2

[0041] 1. Preparation of microbial fertilizer

[0042] Preparation of seed solution: activate the cotton endophytic fungus CEF08111 on a PDA plate, pick a small amount of hyphae and inoculate it in Czapek liquid medium after 1 week, culture at 25°C, 150r / min, for 5 days, and the conidia concentration is greater than 1×10 7 per mL, the suspended spore liquid is the seed liquid at this time;

[0043] Preparation of microbial strains: add 0.25Kg of boiled water to 1.0Kg of corn flour and mix well, soak for 30min, add 0.35Kg of sand, mix well, put into polypropylene strain bags (15×55cm), each bag contains 0.5Kg, Seal with double sponge rings, autoclave for 30 minutes to obtain corn sand medium; after cooling to room temperature, the concentration of each bag is 1×10 7 15mL of suspended spore liquid per mL, mixed evenly and placed in a 25°C culture room for fermentation for 7 to 9 days, so that the yield of conidia reached 10 8 pcs / g.

[0044] Preparation of microbial fertiliz...

Embodiment 3

[0048] Verticillium wilt control of embodiment 3 cotton verticillium wilt serious disease field

[0049] 1. Selection of cotton fields

[0050] In the present invention, the disease-resistant variety Zhongmiansuo 49 is used as the test variety, and the cotton seeds are selected after delinting with sulfuric acid, and then coated with 2.5% fludioxonil suspension seed coating agent, and the amount of seed coating agent used is 30ml of active ingredients / 100 kg of seeds.

[0051] In Aksu, Xinjiang, one cotton field was selected for continuous cropping of cotton for more than 20 years, 10 years, and 5 years, and they were named Aksu A, Aksu B, and Aksu C respectively. Five plots were selected for each plot, and the disease-resistant variety Zhongmiansuo 49 was planted. The planting area of ​​each plot is 100m 2 , sown in mid to late April.

[0052] The selected fields are routinely applied chemical fertilizers, N, P 2 o 5 、K 2 The total amount of O is 25, 10, 10 kg / mu respe...

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Abstract

The invention belongs to the field of agricultural biotechnologies, and particularly relates to a method for preventing and treating verticillium wilt of cotton verticillium wilt seriously diseased fields. The method includes that disease-tolerant varieties, microbial organic fertilizers and resistance inducers are jointly used, national or provincial-level authorized cotton varieties with verticillium wilt disease indexes lower than 30 are adopted, bacterial strains CEF08111 are fermented to obtain the microbial bacterial fertilizers, the application amounts are 4 m<3>, and basal applicationis carried out; the resistance inducers are a type of exogenous biological or non-biological substances capable of inducing cotton plants to develop resistance to target diseases. Application processes include carrying out leaf surface spraying in pathogenic initial phases. The method has the advantages that the method is green, environmentally friendly and sustainable and is high in efficiency, synergistic control effects can be realized for the cotton verticillium wilt, and yield loss due to diseases can be remarkable abated.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for preventing and treating Verticillium wilt in cotton fields seriously ill with Verticillium wilt. Background technique [0002] Verticillium dahliae caused by Verticillium dahliae is a devastating disease in cotton production. Since the 1990s, the disease has caused disasters in major cotton areas in my country, with an annual occurrence area of ​​about 3 million hectares and a yield loss of about 100 million kilograms of lint, and the fiber quality of cotton is seriously affected. Therefore, Verticillium wilt is one of the important limiting factors for the development of cotton production in my country. Verticillium dahliae is the pathogen of Verticillium dahliae in cotton. It survives in the soil for a long time in the form of microsclerotia or mycelium. It invades from the root of the plant and spreads along the vascular bundle. It is a typical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01C1/06C05G3/00C05F17/00C12N1/14C12R1/645
CPCY02W30/40
Inventor 朱荷琴冯自力冯鸿杰魏锋师勇强赵丽红周京龙张一豪
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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