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Preparation method for microecological preparation used for preventing and treating reproductive tract infection of dairy cow

A micro-ecological preparation and reproductive tract infection technology, which is applied in the direction of anti-infective drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems that have not been reported, and achieve obvious antagonism effects, simple and reasonable steps, and suitable scale The effect of chemical production

Active Publication Date: 2013-05-01
内蒙古双奇药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there is a certain understanding of the composition of the vaginal flora of dairy cows, there are no reports on its application in the prevention and treatment of reproductive tract infections in dairy cows

Method used

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  • Preparation method for microecological preparation used for preventing and treating reproductive tract infection of dairy cow
  • Preparation method for microecological preparation used for preventing and treating reproductive tract infection of dairy cow
  • Preparation method for microecological preparation used for preventing and treating reproductive tract infection of dairy cow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Isolation and identification of dominant lactic acid bacteria in the vagina of dairy cows

[0027] Firstly, through material collection, colony counting, bacteria isolation and data analysis, the distribution of bacteria in the vagina of dairy cows under healthy and diseased conditions was preliminarily determined. Fifty-five strains of lactic acid bacteria were isolated through the differential medium, and the isolated lactic acid bacteria were screened again, and 8 strains of better lactic acid bacteria were selected for physiological, biochemical and molecular biology identification. The best two strains of lactic acid bacteria, the biological identification results of these two strains of lactic acid bacteria are as follows.

[0028] For the convenience of the public, the two strains of lactic acid bacteria can be purchased through domestic commercial channels, namely SQ00048 and SQ00054 lactic acid bacteria of Shuangqi Company.

[0029] (1) Biological i...

Embodiment 2

[0066] Embodiment 2. Detection of antagonistic effect of SQ00048 and SQ00054 lactic acid bacteria

[0067] The safety experiments of these two strains were carried out in mice to detect their non-toxicity. At the same time, the antagonism of SQ00048 and SQ00054 lactic acid bacteria to common pathogenic bacteria in reproductive tract infections was tested, and the lactic acid bacteria and pathogenic bacteria were mixed and cultivated according to a certain ratio. The number of viable bacteria in the -8h growth curve is used to determine the inhibitory effect of probiotics on pathogenic bacteria based on the results of the number of viable bacteria in the two.

[0068] When SQ00048 is mixed with Escherichia coli and Staphylococcus aureus, which are common in reproductive tract infections, in different proportions, Escherichia coli and Staphylococcus aureus are significantly inhibited, see Figure 1-2 ; When SQ00054 was mixed with common Escherichia coli and Staphylococcus aureus...

Embodiment 3

[0072] Embodiment 3. the production technology of bacterial strain bacterial powder

[0073] Lactic acid bacteria SQ00048 and SQ00054 from Shuangqi Company were selected as the main strains of the preparation.

[0074] 1, the preparation method of SQ00048 strain bacterial powder

[0075] The optimal fermentation time of SQ00048 is 8 hours, the fermentation temperature is 40°C, and anaerobic culture;

[0076] The optimal fermentation medium for SQ00048: 1.2% lactose, 0.8% beef extract, 0.4% triamine citrate, 0.4% potassium acetate, 0.04% magnesium sulfate, 0.05% L-cysteine ​​hydrochloride, 0.08% Tween -80.

[0077] Centrifuge the obtained fermented liquid at 15000g for 30 minutes, discard the supernatant to obtain a wet bacterial sludge, add a protective agent, stir evenly, pour it into a freeze dryer and freeze-dry to obtain a white powdery bacterial powder.

[0078] Protective agent composition and addition ratio are:

[0079] Name Concentration (%) Addition amount ( / 300m...

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Abstract

The invention aims to provide a microecological preparation capable of preventing and treating reproductive tract infection of a dairy cow. Dominating lactic acid bacteria in the vagina of a healthy dairy cow are separated and identified; then two optimal lactic acid bacteria are selected; the two lactic acid bacteria are non-toxic according to results of safety detection on mice and have striking antagonistic effects according to results of detection on their antagonism on pathogenic bacteria. The two lactic acid bacteria are used as raw materials and fermented to prepare bacterial powders, then the bacterial powders of the two lactic acid bacteria are mixed together, and an obtained mixture is returned to the body of an ill animal; colonization of bacterial strains is benefited, an inhibitory effect is exerted on pathogenic bacteria, damage of chemical medicines to a body is avoided, the problem of residual medicines in the body is overcome, and the dairy cow is benefited in production of a dam.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to a microecological preparation that can prevent and treat reproductive tract infections in dairy cows. It has the characteristics of antibacterial, non-toxic, no side effects, no residue, and is easy to colonize the reproductive tract. The probability of livestock, can be widely used in the prevention and treatment of reproductive tract infection in dairy cows. Background technique [0002] Clinically, due to the physiological and pathological damage of the cow's vagina, the infection of the reproductive tract of dairy cows and the secondary genital tract lesions are very common. To prevent and treat reproductive tract diseases, the current domestic veterinary drug market uses chemically synthesized drugs. Although the therapeutic effect is remarkable, it has led to drug resistance in animals, and drug residues in animal products have caused serious damage to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P15/02A61P31/00A61K35/744
Inventor 黄少磊刘彦民龚虹付艳茹刘斌冯谦夏少明张春霞邰海欧
Owner 内蒙古双奇药业股份有限公司
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