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Novel fermentation production method of saccharomycopsis fibuligera

A technology of buttoning capsule-coating and fermentation methods, which is applied in the field of fermentation and production of new button-coating-coating yeast, to achieve the effects of reducing the inactivation of live bacteria, low operation difficulty, and a large number of viable bacteria

Inactive Publication Date: 2017-06-06
广东瑞丰生态环境科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the researches on Saccharomyces spp. are only in the auxiliary optimization of the brewing industry, and there is no research on its large-scale production. With the deepening of the research on the physiological characteristics of S. The role of yeast Fumembrane is gradually known by people, and the demand for Yeast Fumembrane is increasing. Therefore, it is necessary to find a production method that can carry out fermentation of Yeast Fumembrane in batches

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of liquid seed medium: Through medium optimization experiments, the optimal liquid seed medium for cultivating denitrifying bacteria was obtained. Starch 20g, water 1L;

[0026] (2) Seed preparation: first conduct conventional strain screening and activation, then inoculate in the optimized liquid seed medium, and cultivate in the incubator for 40 hours to obtain liquid seeds in the logarithmic phase;

[0027] (3) Preparation of solid plate: Add 24g of agar to each 1L of yeast liquid seed medium, sterilize it and pour it into the plate, and after cooling, it can be made into a solid plate medium containing liquid medium components ;

[0028] (4) Scale-up fermentation: Spray the liquid seeds obtained in step (2) evenly on the surface of the solid plate medium, and then place it in a constant temperature fermentation room at 35°C for closed fermentation and culture for 30 hours;

[0029] (5) Collecting the sludge: After the fermentation is over, take the...

Embodiment 2

[0032] (1) Preparation of liquid seed medium: Through medium optimization experiments, the optimal liquid seed medium for cultivating denitrifying bacteria was obtained. The proportion of the medium was: yeast extract 8g, bacteriological peptone 22g, malt powder 22g, Starch 18g, water 1L;

[0033] (2) Seed preparation: first conduct conventional strain screening and activation, then inoculate in the optimized liquid seed medium, and cultivate in the incubator for 36 hours to obtain liquid seeds in the logarithmic phase;

[0034] (3) Preparation of solid plate: Add 18g of agar to every 1L of yeast liquid seed medium, sterilize it and pour it into the plate. After cooling, it can be made into a solid plate medium containing liquid medium components. ;

[0035] (4) Enlarged fermentation: Spray the liquid seeds obtained in step (2) evenly on the surface of the solid plate medium, and then place it in a constant temperature fermentation room at 35°C for closed fermentation and cul...

Embodiment 3

[0039] (1) Preparation of liquid seed medium: Through medium optimization experiments, the optimal liquid seed medium for cultivating denitrifying bacteria was obtained. The proportion of the medium was: yeast extract 12g, bacteriological peptone 18g, malt powder 18g, Starch 22g, water 1L;

[0040] (2) Seed preparation: perform conventional strain screening and activation first, then inoculate in the optimized liquid seed medium, and cultivate in the incubator for 48 hours to obtain liquid seeds in the logarithmic phase;

[0041] (3) Preparation of solid plate: Add 32g of agar to each 1L of liquid seed culture medium of Saccharomyces fumigatus, pour it into the plate after sterilization, and make a solid plate medium containing liquid medium components after cooling ;

[0042] (4) Scale-up fermentation: Spray the liquid seeds obtained in step (2) evenly on the surface of the solid plate medium, and then place it in a constant temperature fermentation room at 35°C for closed f...

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Abstract

The invention discloses a production method of saccharomycopsis fibuligera. The method comprises the following steps: (1) preparing a liquid seed culture medium: obtaining the optimal liquid seed culture medium for cultivating denitrifying bacteria through a culture medium optimizing experiment; (2) preparing seeds: performing conventional bacteria screening and activation, and performing cultivation in the liquid seed culture medium to obtain liquid seeds in a logarithmic phase; (3) preparing a solid flat plate: performing a solid flat plate culture medium containing liquid culture medium components; (4) performing amplification fermentation: spraying the liquid seeds obtained in the step (2) on the surface of the solid flat plate and performing closed fermentation cultivation in a fermentation chamber; (5) collecting bacterial sludge; (6) preparing bacterial powder. By the production method, the saccharomycopsis fibuligera can be produced on a large scale, the saccharomycopsis fibuligera has a large number of viable bacteria, the production process is low in operation difficulty, the whole utilization rate of raw materials is high, the production cost is low and the production efficiency is high.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular, the invention relates to a fermentation production method of a novel vesicular complex membrane yeast. Background technique [0002] Industrial biotechnology overcomes the energy and environmental crisis caused by traditional chemical processing methods, and is attracting increasing attention. Starch is the main raw material of industrial biotechnology, and it is a renewable natural resource with abundant sources and low price. Saccharomycopsis fibuligera, also known as Endomycopsis fibuligera, can express raw starch liquefaction enzyme and glucoamylase, and is considered to be one of the best ascomycete yeasts for producing amylolytic enzymes. The biofermentation by the yeast Fumembrane can directly process starch into various products that people need without liquefaction and saccharification, which can save energy and reduce costs, which is of great significance ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/20C12P19/14C12R1/645
CPCC12N1/16C12P19/14C12P19/20
Inventor 朱爱军张子曦洪嘉琦陈秋雄
Owner 广东瑞丰生态环境科技股份有限公司
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