Preparation method of microecological agent capable of reducing human body serum cholesterol content

A technology of probiotics and cholesterol, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, metabolic diseases, etc., can solve the problems of complex composition of protective agents and unsatisfactory effects, and achieve suitable large-scale production, low cost, Practical effect

Active Publication Date: 2013-04-03
内蒙古双奇药业股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] These existing technologies are all improved on the protective agent. The composition of the protective agent is complex, and its final effect is

Method used

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  • Preparation method of microecological agent capable of reducing human body serum cholesterol content
  • Preparation method of microecological agent capable of reducing human body serum cholesterol content
  • Preparation method of microecological agent capable of reducing human body serum cholesterol content

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1. Production process of Bifidobacterium longum NQ-1501 strain powder

[0033] The Bifidobacterium longum NQ-1501 strain of Shuangqi Company was selected as the only strain of the preparation. The best fermentation time of Bifidobacterium longum NQ-1501 strain is 8h, the fermentation temperature is 40℃, and the culture is anaerobic;

[0034] The best fermentation medium for Bifidobacterium longum strain NQ-1501: 1.2% lactose, 0.8% beef extract, 0.4% triamine citrate, 0.4% potassium acetate, 0.04% magnesium sulfate, 0.05% L-cysteine ​​salt Salt, 0.08% Tween-80.

[0035] Centrifuge the obtained fermentation broth at 15,000 g for 30 minutes, discard the supernatant to obtain a wet bacterial sludge, add a protective agent, stir evenly, and pour into a freeze dryer to freeze-dry to obtain a white powdery bacterial powder.

[0036] The protective agent composition and addition ratio are:

[0037] Name Concentration (%) Addition amount ( / 300ml)

[0038] Skimmed milk powder 50% ...

Example Embodiment

[0040] Example 2. Preparation of a microecological preparation that can be stored at room temperature to reduce human serum cholesterol content

[0041] The Bifidobacterium longum NQ-1501 powder and the auxiliary material starch are thoroughly mixed according to the ratio of 1:9 to make tablets, capsules and powders.

[0042] a. Mix Bifidobacterium longum NQ-1501 powder and auxiliary material starch in a ratio of 1:9, and use a tablet press to form flat tablets on both sides. The average tablet weight is 0.4g, and the tablet weighs 1g. Live bacteria The number is about 20 billion, and the dosage for adults is 2 tablets a day, and a course of treatment is 30 days.

[0043] b. Mix Bifidobacterium longum NQ-1501 powder and auxiliary material starch in a ratio of 1:9, and put them in a capsule in a cylindrical shell made of gelatin. The volume of the capsule is 0.48ml. , The number of viable bacteria per capsule is about 20 billion, and the dosage for adults is 2 capsules per day, and 3...

Example Embodiment

[0047] Example 3. In vitro verification test of cholesterol-lowering function of Bifidobacterium longum NQ-1501 strain

[0048] 1. Experimental materials

[0049] 1. Test strain: Bifidobacterium longum NQ-1501 strain owned by Shuangqi Pharmaceutical.

[0050] 2. Main reagents

[0051] Including bovine bile salt, cholesterol, o-phthalaldehyde, n-hexane, glacial acetic acid, potassium hydroxide

[0052] 3. Preparation of Cholesterol Reducing Screening Medium

[0053] Cholesterol liquid medium for suitable strains: 0.3% (W / V) bovine bile salt was added to TPY liquid medium, and after sterilization at 121°C for 20 minutes, 2% (1.0g / 100ml) sterile cholesterol solution was added. (Cholesterol dissolving solution preparation: accurately weigh 1.0g cholesterol and dissolve it in absolute ethanol and use a 100ml volumetric flask to make the volume constant, filter with a bacteria filter to obtain 1.0g / 100ml cholesterol solution)

[0054] 4. Preparation of o-phthalaldehyde solution

[0055] Accurat...

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Abstract

The invention discloses a preparation method of a microecological agent capable of reducing human body serum cholesterol content. The microecological preparation can be stored for 12 months in an ambient temperature condition. The method comprises the steps of preparing bifidobacterium longum NQ-1501 bacterial strain into bacterial powder, and then mixing the bacterial powder and auxiliary material into the preparation. The preparation can achieve the purposes that only cholesterol is reduced and the absorption of other nutritious substance is not affected, and overcomes the disadvantage that microecological preparation can not be stored in low temperature condition.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to a probiotic preparation that can reduce the cholesterol content in human serum, which has the characteristics of non-toxicity, no side effects, no residue, and easy intestinal colonization. The preparation can also be stored at room temperature for 12 days months. Background technique [0002] High serum cholesterol levels are one of the main causes of cardiovascular diseases that lead to high human mortality in the world. Usually, traditional drug therapy has a single effect and many side effects. However, reducing or reducing the intake of exogenous cholesterol can At present, the measures commonly used to regulate the high cholesterol level in the body; the probiotics can achieve the effect of simply reducing cholesterol without affecting the absorption of other nutrients while exerting many functions such as regulating the intestinal tract, so it has att...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P3/06A61K35/745
Inventor 黄少磊刘彦民张春霞刘斌王春颖李康宁程超冯谦龚虹马晓红李晓军李云旭韩志邰海欧
Owner 内蒙古双奇药业股份有限公司
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