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Preparation and application of efficient immunoregulation micro-ecological preparation

A technology of micro-ecological preparations and immune regulation, applied in the direction of microorganism-based methods, applications, microorganisms, etc., can solve the problems of ineffective use, low bacterial survival rate, and low total number of viable bacteria, so as to increase non-specific immunity and improve The total number of live bacteria and the effect of improving immunity

Pending Publication Date: 2022-01-28
重庆优宝生物技术股份有限公司
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  • Abstract
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Problems solved by technology

[0004] In order to make up for the deficiencies of the prior art and solve the problems that the total number of viable bacteria in the culture medium is not high, the survival rate of bacteria is low, and the use effect is not obvious in the microecological preparations prepared by the existing preparation methods, a method proposed by the present invention Preparation and application of high-efficiency immunomodulatory microecological preparations

Method used

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  • Preparation and application of efficient immunoregulation micro-ecological preparation
  • Preparation and application of efficient immunoregulation micro-ecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of preparation of high-efficiency immunomodulatory probiotics, the preparation method comprises the following steps:

[0023] S1: The ratio of raw materials is: glucose 0.5-1.0%, isomaltose oligosaccharide 0.1-0.5%, soybean peptone 0.1-0.5%, diammonium hydrogen citrate 0.1%, sodium acetate 0.1%, K 2 HPO 4 0.1%, MgSO 4 0.015%, MnSO 4 0.02%, L-cysteine ​​hydrochloride 0.025% and water to prepare Lactobacillus acidophilus fermentation medium;

[0024] S2: Add lactic acid or NaOH to the Lactobacillus acidophilus fermentation medium, so that the pH range of the Lactobacillus acidophilus fermentation medium is 6.0-6.4, prepare the fermentation medium, and finally sterilize it by high-pressure steam at 121°C for 30 minutes;

[0025] S3: cooling the fermentation medium obtained through S2 to 37° C. with iced brine;

[0026] S4: inoculate the Lactobacillus acidophilus strain cultivated by MRS (biochemical reagent) into the fermentation medium, the inoculum amount is 0...

Embodiment 2

[0029] S1: The ratio of raw materials is: glucose 0.5-1.0%, isomaltose oligosaccharide 0.1-0.5%, soybean peptone 0.1-0.5%, diammonium hydrogen citrate 0.1%, sodium acetate 0.1%, K 2 HPO 4 0.1%, MgSO 4 0.015%, MnSO 4 0.02%, L-cysteine ​​hydrochloride 0.025%, Yunzhi glycopeptide 0.01-0.05%, astragalus polysaccharide 0.01-0.05%, triterpene saponin 0.01-0.05% and water to prepare Lactobacillus acidophilus fermentation medium ;

[0030] S2: Add lactic acid or NaOH to the Lactobacillus acidophilus fermentation medium, so that the pH range of the Lactobacillus acidophilus fermentation medium is 6.0-6.4, prepare the fermentation medium, and finally sterilize it by high-pressure steam at 121°C for 30 minutes;

[0031] S3: cooling the fermentation medium obtained through S2 to 37° C. with iced brine;

[0032] S4: inoculate the Lactobacillus acidophilus strain cultivated by MRS (biochemical reagent) into the fermentation medium, the inoculum amount is 0.05-0.1%;

[0033] S5: Infuse ...

Embodiment 3

[0039] Application of probiotics in broilers:

[0040] A total of 90 1-day-old newborn chickens from the same batch were selected and divided into 3 groups with 30 chickens in each group.

[0041] The control group used chicken farm well water without addition as drinking water.

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Abstract

The invention belongs to the field of microecological preparations, and particularly relates to preparation and application of an efficient immunoregulation micro-ecological preparation. The preparation method comprises the following steps of: S1, preparing a lactobacillus acidophilus fermentation culture medium from the following raw materials in percentage by weight: 0.5-1.0% of glucose, 0.1-0.5% of isomaltooligosaccharide, 0.1-0.5% of soy peptone, 0.1% of diammonium hydrogen citrate, 0.1% of sodium acetate, 0.1% of K2HPO4, 0.015% of MgSO4, 0.02% of MnSO4, 0.025% of L-cysteine hydrochloride, 0.01-0.05% of polysaccharopeptide, 0.01-0.05% of astragalus polysaccharide, 0.01-0.05% of triterpenoid saponin and water; S2, adding lactic acid or NaOH into the lactobacillus acidophilus fermentation culture medium to enable the pH range of the lactobacillus acidophilus fermentation culture medium to be 6.0-6.4, preparing a fermentation culture medium, and finally, conducting sterilization for 30 min through high-pressure steam at the temperature of 121 DEG C; and S3, cooling the fermentation culture medium obtained in S2 to 37 DEG C by using brine ice. The total number of viable bacteria of lactobacillus acidophilus can be remarkably increased, and meanwhile, the inhibition effect on harmful bacteria (escherichia coli and salmonella) is very remarkable.

Description

technical field [0001] The invention belongs to the field of microecological preparations, in particular to the preparation and application of a high-efficiency immune-regulating microecological preparation. Background technique [0002] Lactobacillus acidophilus is one of the 34 kinds of probiotics approved by the Ministry of Agriculture in 2013 to be used as feed additives. Belonging to the genus Lactobacillus, it is an important microorganism in the intestinal tract. As one of the beneficial flora of the body and one of the commonly used strains for preparing microecological preparations, when it reaches a certain amount, it can regulate the intestinal flora, inhibit pathogenic bacteria, and enhance immunity. Strength, blood fat lowering and other effects, play a health-promoting effect. Lactobacillus acidophilus has become one of the probiotics that attaches great importance to research and development in the current lactic acid bacteria family. [0003] The microecolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75C12R1/23
CPCC12N1/20A23K10/18A23K50/75
Inventor 钟世才孟怀旺任丽
Owner 重庆优宝生物技术股份有限公司
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