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Fermentation method for lactobacillus with high cell density

A technology of lactic acid bacteria fermentation and lactic acid bacteria, which is applied in the field of lactic acid bacteria fermentation with high cell density, can solve the problems of high cost of lactic acid bacteria products, high cost of lactic acid bacteria, and long fermentation time, so as to improve product quality, increase the number of viable bacteria, and prepare simple effect

Active Publication Date: 2015-05-06
BIOFORTE BIOTECHNOLOGY (SHENZHEN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the lactic acid bacteria produced by the current traditional fermentation process have the disadvantages of low bacterial concentration, long fermentation time, complicated process, and low survival rate, which makes the cost of fermented lactic acid bacteria relatively high, and also makes the cost of lactic acid bacteria products remain high. Become a bottleneck problem in the lactic acid bacteria fermentation industry

Method used

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  • Fermentation method for lactobacillus with high cell density
  • Fermentation method for lactobacillus with high cell density

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] S1. Construct fiber bed reactor; figure 1 shown. attached figure 1 A structural schematic diagram of a fiber bed reactor is provided, including a reactor body 3, a heating device 9 is provided outside the reactor body 3, and a feed port 1, a sampling port 2, and an acid-base feed are provided at the upper end of the reactor body 3 System 6, the lower end of the reactor main body 3 is provided with a discharge port 11, a fiber material 10 is fixed inside the reactor main body 3, a stirring device 4, a pH electrode 7, a temperature electrode 8 are arranged inside the reactor main body 3, and an acid-base feeding system 6 , pH electrode 7 and temperature electrode 8 are connected with the control system 5, and the pH and temperature can be fed back to the control system 5 in real time, and the automatic control is realized through the acid-base feeding system 6 and the heating system 9.

[0031] The medium described in S2 is: 3% hydrolyzed whey protein (g / L) (food grade)...

Embodiment 2

[0037] S1. Build fiber bed reactor; Same as Example 1;

[0038] The medium described in S2 is: 3% hydrolyzed whey protein (g / L) (food grade), 2-2.5% glucose (g / L), 0.5-1% magnesium sulfate heptahydrate (g / L) , 10% corn steep liquor (ml / L), distilled water to a volume of 1L to prepare a culture medium, add it to the fiber bed reactor, and sterilize at 121°C for 15-20 minutes before use;

[0039] S3. Into the culture medium, insert Lactobacillus bulgaricus and Streptococcus thermophilus seed liquid activated by the MRS liquid medium, and the inoculation amount is 3% and 3% of the total volume of the culture medium respectively;

[0040] S4. Adjust the temperature to 37°C, adjust the pH value to 6.5, carry out fermentation culture, and regularly take samples to detect the number of bacteria;

[0041] S5. When the number of bacteria reaches 10 10 Stop fermentation when cfu / ml is above. All the fermentation broth is discharged from the outlet and collected for downstream process...

Embodiment 3

[0044] S1. Build fiber bed reactor; Same as Example 1;

[0045] The medium described in S2 is: 3% hydrolyzed whey protein (g / L) (food grade), 2-2.5% glucose (g / L), 0.5-1% magnesium sulfate heptahydrate (g / L) , 10% corn steep liquor (ml / L), distilled water to a volume of 1L to prepare a culture medium, add it to the fiber bed reactor, and sterilize at 121°C for 15-20 minutes before use;

[0046] S3. Insert the seed solution of Lactobacillus acidophilus, Lactobacillus bulgaricus and Streptococcus thermophilus activated by the MRS liquid medium into the culture medium, and the inoculum amount is 2%, 2% and 2% of the total volume of the culture medium respectively;

[0047] S4. Adjust the temperature to 37°C, adjust the pH value to 6.4, carry out fermentation culture, and regularly take samples to detect the number of bacteria;

[0048] S5. When the number of bacteria reaches 10 10 Stop fermentation when cfu / ml is above. All the fermentation broth is discharged from the dischar...

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Abstract

The invention relates to the technical field of biological products, and discloses a fermentation method for lactobacillus with high cell density. The fermentation method comprises the steps of constructing a fibrous bed reactor, preparing a culture medium, inoculating, culturing by fermenting, detecting microbial content, collecting fermentation liquor and the like. According to the fermentation method, fiber materials are successfully arranged inside the reactor as carriers of immobilized lactobacillus cells, and the number of the lactobacillus cells can be substantially enhanced in fermentation of lactobacillus with the fibrous bed and under specific fermentation conditions. The biological reactor can be separately used or be taken as a seed fermentation tank to be combined with a common reactor for carrying out sequence fermentation in batches or continuous fermentation, and has the characteristics of being high in production efficiency and stable in batch production, shortening a fermentation period, ensuring high cell density and the like; fiber materials used by the method are cheap; the device is simple and convenient to operate, the production cost is low, and the fermentation method can be well applied to industrialized production of cells of lactobacillus such as lactobacillus acidophilus, lactobacillus bulgaricus and bifidobacterium.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a method for fermenting lactic acid bacteria with high cell density. technical background [0002] Lactic acid bacteria are a common type of probiotics in the intestinal tract of animals. They can improve the micro-ecological balance in the intestinal tract of animals, inhibit the growth of pathogenic bacteria, promote digestion, and improve human immunity. At present, there are a wide variety of products using it as an active ingredient, which has also stimulated the rise of the lactic acid bacteria fermentation industry. However, the lactic acid bacteria produced by the current traditional fermentation process have the disadvantages of low bacterial concentration, long fermentation time, complicated process, and low survival rate, which makes the cost of fermented lactic acid bacteria relatively high, and also makes the cost of lactic acid bacteria products remain h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/23C12R1/225C12R1/46C12R1/01
Inventor 胡文锋庞旭韩笑刘小阳杨虹坤刘秉杰杨益衡
Owner BIOFORTE BIOTECHNOLOGY (SHENZHEN) CO LTD
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