Animal selenium-enriched health-care nutritional liquid, preparation method and application

A health-care nutrient solution and selenium-enriched technology, which is applied in applications, animal feed, animal feed, etc., to reduce the occurrence of diseases, improve immunity and resistance, and reduce the harm caused by the environment and human body

Inactive Publication Date: 2017-08-29
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this has not completely broken the chain of antibiotics threatening health, because the abuse of antibiotics in animals is threatening human health

Method used

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  • Animal selenium-enriched health-care nutritional liquid, preparation method and application
  • Animal selenium-enriched health-care nutritional liquid, preparation method and application
  • Animal selenium-enriched health-care nutritional liquid, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The concrete components of the selenium-enriched animal health care nutrient solution of the present embodiment are as follows:

[0040]

[0041]

[0042] The preparation method of the present embodiment is as follows:

[0043] (1) Breeding of selenium-enriched yeast strains

[0044] Candida utilis was cultured on a YEPD culture plate for 24 hours, and a single colony was picked into a 250ml Erlenmeyer flask containing 100ml YEPD liquid medium, 180rpm, shaking culture for 36 hours, and the bacteria liquid was centrifuged and washed 3 times to make a single cell suspension. solution, so that the cell concentration is 10 8 ~10 9individual / mL. Take 0.1mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it on a clean bench to air dry to form a bacterial film. in N + Ion implantation is carried out in the implanter with an energy of 18KeV. Six gradient doses were made for each strain, with two plates for each dose. N + The inj...

Embodiment 2

[0065] The specific components of the selenium-enriched animal health care nutrient solution of the present embodiment are as follows:

[0066]

[0067]

[0068] The preparation method of the present embodiment is as follows:

[0069] (1) Breeding of selenium-enriched yeast strains is the same as in Example 1.

[0070] (2) Yeast hydrolyzate obtained

[0071] The above-mentioned Candida utilis cas15 was inoculated in 500 mL of fermentation medium, and cultured with shaking at 28° C. and 170 rpm for 60 h to obtain a selenium-enriched yeast culture. The composition of the fermentation medium is: malt extract 15g / L, peptone 12g / L, yeast extract 8g / L, sodium selenite 150mg / L.

[0072] Collect the selenium-enriched yeast culture, centrifuge at 9000rpm, centrifuge for 10 minutes, wash, add a mixed enzyme preparation of wall-breaking enzyme and papain in a mass ratio of 1:1, and enzymatically hydrolyze for 20 hours to obtain organic selenium and amino acids. of yeast hydrolyz...

Embodiment 3

[0090] The specific components of the selenium-enriched animal health care nutrient solution of the present embodiment are as follows:

[0091]

[0092] The preparation method of the present embodiment is as follows:

[0093] (1) Breeding of selenium-enriched yeast strains is the same as in Example 1.

[0094] (2) Yeast hydrolyzate obtained

[0095] The above-mentioned Candida utilis cas15 was inoculated in 500 mL of fermentation medium, and cultured with shaking at 28° C. and 180 rpm for 72 hours to obtain a selenium-enriched yeast culture. The composition of the fermentation medium is: malt extract 20g / L, peptone 15g / L, yeast extract 10g / L, sodium selenite 180mg / L.

[0096] Collect the selenium-enriched yeast culture, centrifuge in a centrifuge, 10000rpm, centrifuge for 10min, wash, add a mixed enzyme preparation of wall-breaking enzyme and papain in a mass ratio of 1:1, and enzymatically hydrolyze for 24 hours to obtain organic selenium and amino acids of yeast hydrol...

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Abstract

The invention discloses an animal selenium-enriched health-care nutritional liquid, a preparation method and an application. The preparation method of the animal selenium-enriched health-care nutritional liquid comprises breeding of a selenium-enriched yeast strain, acquisition of a yeast hydrolysate, fermentation of a single probiotics strain, preparation of a probiotics mixed liquid, and compounding of the selenium-enriched yeast hydrolysate and probiotics. The preparation method specifically comprises the following steps of: adding selenium-enriched yeast hydrolysate dry powder into the probiotics mixed liquid in a proportion of 10-15g / L; and adding a fresh culture medium into the compound mixed liquid, adding potassium sorbate into the probiotics mixed liquid, the mass concentration of which is 0.08-0.1%, into the probiotics mixed liquid, and uniformly mixing the mixture to obtain the animal selenium-enriched health-care nutritional liquid. According to the animal selenium-enriched health-care nutritional liquid provided by the invention, five probiotics strains are separately fermented and are combined according to the optimum proportion, and the probiotics strains are synergistic, so that the animal selenium-enriched health-care nutritional liquid has the characteristics of novel strain combination, stable structure, high enzyme activity, great viable count and etc. Meanwhile, by adding the hydrolysate of bred highly selenium-enriched yeast into the nutritional liquid, and the hydrolysate and probiotics are organically combined, so that the growth-promoting and life-preserving effects of organic selenium and probiotics are exerted.

Description

technical field [0001] The invention relates to an animal feed additive, in particular to an animal selenium-enriched health care nutrient solution, a preparation method and application. Background technique [0002] With the development of my country's livestock and poultry breeding industry, the large-scale and intensive livestock and poultry breeding industry has risen rapidly. Drug resistance due to the abuse of antibiotics will make human beings fall into a hopeless nightmare. For this reason, the state has stipulated more than 400 antibiotics Medicines must be purchased with a prescription from the hospital. However, this has not completely cut off the chain of antibiotics threatening health, because the abuse of antibiotics in animals is threatening human health. [0003] At the same time, the concept of animal welfare breeding has gradually been recognized and accepted by the majority of farmers, especially the managers of large-scale farms. Welfare breeding refers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/16A23K10/18A23K20/20A23K50/30A23K50/60
CPCA23K10/16A23K10/18A23K20/30A23K50/30A23K50/60
Inventor 黄胜威吴丽芳王钰婷蔡冬清吴正岩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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