Phellinus igniarius bacterial strain liquid fermenting culture medium and method for fermenting and producing phellinus linteus polysaccharides

A liquid fermentation and culture medium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of scarce supply, high price, difficult to find, etc., and achieve the effect of improving immunity

Inactive Publication Date: 2013-01-16
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the extremely high requirements on the growth environment, temperature, and humidity of Phellinus, and the long growth cycle, it is extremely difficult to find, so the natural Phellinus is very rare, the supply is sc...

Method used

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  • Phellinus igniarius bacterial strain liquid fermenting culture medium and method for fermenting and producing phellinus linteus polysaccharides
  • Phellinus igniarius bacterial strain liquid fermenting culture medium and method for fermenting and producing phellinus linteus polysaccharides
  • Phellinus igniarius bacterial strain liquid fermenting culture medium and method for fermenting and producing phellinus linteus polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Embodiment one: select ph.l 0003+1 Phellinus strains were tested. Different nitrogen sources (beef extract 4 g / L, peptone 4 g / L, yeast powder 4 g / L, soybean powder 50 g / L, corn flour 50 g / L, bran 50 g / L, ammonium tartrate 0.32 g / L, ammonium sulfate 0.32 g / L, sodium nitrate 0.32 g / L), carbon source (glucose 20 g / L, maltose 20 g / L, fructose 20 g / L, sucrose 20 g / L, lactose 20 g / L L, corn flour 20 g / L, rice flour 20 g / L, soluble starch 20 g / L), carbon-nitrogen ratio (1:1, 3:1, 5:1, 8:1, 10:1, 12: 1, 20:1), inorganic salt (KH 2 PO 4 1.0 g / L, MgSO 4 ·7H 2 O 0.5 g / L, FeSO 4 0.1 g / L, ZnSO 4 0.1 g / L, MnSO 4 0 . 1 g / L, CaCl 2 0.5 g / L) and vitamins (thiamine 10 mg / L, riboflavin 10 mg / L, nicotine acid 10 mg / L, folic acid 10 mg / L, ascorbic acid 10 mg / L, biotin 10 mg / L) Through single factor screening and orthogonal test (Table 5), the optimal liquid fermentation medium formula that is beneficial to mycelium growth and significantly increases the content of intracel...

Embodiment 2

[0094] Example 2: Different temperature (22.5 ℃, 25 ℃, 28 ℃, 30 ℃, 32 ℃, 37 ℃), shaking speed (100 rpm, 120 rpm, 140 rpm, 160 rpm, 180 rpm, 200 rpm), inoculation amount (5%, 10%, 15%, 20%, 25%, 30%), fermentation days (5 d, 6 d, 7 d, 8 d, 9 d, 10 d, 11 d, 12 d) and pH Values ​​(pH 4.5, pH 5.5, pH6.5, pH 7.5, pH 8.5, pH 9.5) were obtained through single factor screening and orthogonal test (Table 6), which are beneficial to mycelium growth and significantly increase the content of intracellular and exopolysaccharides Optimum liquid fermentation conditions for: temperature 28 ℃, shaking speed 160 rpm, inoculum size 25%, culture days 10 days, pH value natural. The mycelium yield reached 9.36 g / L, which was 275.90 % higher than that of the general fungal culture medium, the content of intracellular polysaccharide was 101.18% higher, and the content of extracellular polysaccharide was 23.13% higher.

[0095] Table 6 Orthogonal test factors and levels

[0096]

Embodiment 3

[0097] Example 3: Through the hot water leaching method orthogonal test (Table 7), the best process for extracting Phellinus polysaccharides was obtained: material-to-water ratio 1:30, leaching temperature 90 °C, leaching time 0.5 h, leaching The number of times is 3 times. The obtained Phellinus polysaccharide content is 230.47mg / g, which is 6.34% higher than the polysaccharide extracted by general hot water extraction method

[0098] Table 7 Orthogonal test factors and levels

[0099]

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Abstract

The invention relates to a phellinus igniarius bacterial strain liquid fermenting culture medium and a method for fermenting and producing phellinus linteus polysaccharides. The culture medium has a prescription shown as follows: 20 to 30g/L of glucose, 2 to 6g/L of yeast powder, 1 to 3g/L of KH2PO4, 0.3 to 0.7g/L of MgSO4.7H2O, 110mg/L of VB, and 1L of water; a pH is under a natural condition; a fermenting condition is that a temperature is at 28 to 32 DEG C, a rocking speed is at 140 to 180rpm, inoculation amount is 20 to 30%, cultivation is carried out for 9 to 11 days; the method comprises the following steps of: centrifuging, collecting fermenting liquid, washing via distilled water, precipitating to obtain a mycelium, then extracting polysaccharide inside or outside cells. With the adoption of the phellinus igniarius bacterial strain liquid fermenting culture medium and the method for fermenting and producing the phellinus linteus polysaccharides provided by the invention, yield of the mycelium of phellinus igniarius and content of the polysaccharide inside or outside the cells can be obviously increased; moreover, yields of the phellinus linteus polysaccharides is 6.34% higher than that of polysaccharide extracted by a common hot water extracting method. An animal test proves that extracted phellinus linteus polysaccharides have obvious biological activity such as tumour resistance, oxidization resistance, and improvement of immunity.

Description

technical field [0001] The invention specifically relates to a liquid fermentation medium of a Phellinus japonica strain and a method for fermenting and producing Phellinus japonica polysaccharide. Background technique [0002] Phellinus ( Phellinus igniarius ), the name of a traditional Chinese medicine, which is named for the fact that it usually grows on Morus L. plants and the fruiting body is yellowish brown. It has the reputation of "forest gold". Phellinus is a large and precious perennial medicinal fungus, which uses fruiting bodies as medicine. In my country, Phellinus has been used to treat diseases since the Ming Dynasty and has a history of more than 2,000 years. In ancient times, there was a saying among the folks that "the mulberry tree grows yellow, fortunately, all diseases can be cured" and the legend that "if you get the yellow pimple (Panghuang) attached to the mulberry tree, the dead can also be resurrected". Traditional Chinese medicine believes that P...

Claims

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Application Information

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IPC IPC(8): C05G3/00C12P19/04C12R1/645
Inventor 郑金贵黄缘缘黄志伟谢宝贵
Owner FUJIAN AGRI & FORESTRY UNIV
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