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Ethylparaben-producting fermentation medium of brevibacillus brevis strain and fermentation method

A technology of Bacillus brevis and fermentation medium, applied in the field of microbial engineering, can solve pollution and other problems, achieve the effect of adding research data and avoiding pollution

Inactive Publication Date: 2013-09-04
福建省农业科学院农业生物资源研究所
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Problems solved by technology

At present, most of the production and preparation of ethylparaben are industrialized by chemical synthesis, which causes serious pollution.

Method used

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  • Ethylparaben-producting fermentation medium of brevibacillus brevis strain and fermentation method

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Embodiment Construction

[0017] "Bacillus brevis strain" in the present invention refers to the Chinese invention application number CN201010296437.6 filed by the applicant on September 29, 2010, and the name is "a new strain of Bacillus brevis and its application" The "Bacillus brevis strain FJAT-0809-GLX" disclosed in the "Bacillus brevis strain FJAT-0809-GLX" was deposited in the General Microorganism Center of the China Committee for the Collection of Microorganisms on August 27, 2010, and the preservation number is CGMCC No.4115. In addition, in the article "Analysis of Functional Components of Longan Fresh-keeping Functional Microorganism FJAT-0809-GLX (Brevibacillus brevis FJAT-0809-GLX)" published by Che Jianmei and others (Journal of Fujian Agricultural Sciences, 2012, 27 (10): 1106-1111) It is reported that the Bacillus brevis strain FJAT-0809-GLX has broad-spectrum antibacterial properties, and its main functional component is ethylparaben after isolation and identification; thus it shows th...

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Abstract

The invention provides an ethylparaben-producting fermentation medium of a brevibacillus brevis strain, and also discloses a fermentation method which is simple and easy to operate; wherein the brevibacillus brevis strain is brevibacillus brevis strain FJAT-0809-GLX, and the compositions of the fermentation medium are a soybean cake powder 25.18 g / L, DL-malic acid 29.68 g / L, NaCl 13.18 g / L and a peptone 2 g / L, and prepared with ultrapure water in a pH of 7.0. The fermentation condition of brevibacillus brevis strain FJAT-0809-GLX of the invention is optimized, and thus the ethylparaben-producting capability of brevibacillus brevis strain FJAT-0809-GLX is improved, and the fermentation medium and the fermentation method lay a foundation for separation and purification of ethylparaben, mass production, and exploitation of industrial fermentation production, and further can help to avoid pollution to environment when ethylparaben is prepared by a conventional chemical synthesis method.

Description

【Technical field】 [0001] The invention relates to the technical field of microbial engineering, in particular to a fermentation medium and a fermentation method for producing ethylparaben by Bacillus brevis strains. 【Background technique】 [0002] There are more than 10,000 kinds of biologically active substances produced by microorganisms reported in the world, of which more than 200 kinds have been widely used in the fields of medicine, industry, agriculture, animal husbandry, breeding, food preservation and scientific research, and play an important role. social benefits. Microbial bioactive substances include antibiotics and non-antibiotic bioactive substances. Nearly a hundred new microbial active substances are discovered every year, among which new non-antibiotic bioactive substances are increasing year by year, and exceed antibiotics in number. [0003] Bacillus brevis is a class of Gram-positive or variable, rod-shaped bacteria that move with perinatal flagella and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/62C12R1/01
Inventor 刘波车建美史怀唐建阳
Owner 福建省农业科学院农业生物资源研究所
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