Construction of saccharomyces cerevisiae engineering bacteria generating beta-phenethyl alcohol, strain and application thereof

A technology of Saccharomyces cerevisiae and phenylethanol, applied in application, genetic engineering, fermentation, etc., can solve problems such as differences in 2-PE synthesis ability, achieve the effects of optimizing metabolic pathways, increasing conversion rate and yield, and optimizing metabolic flow

Inactive Publication Date: 2013-11-27
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are large differences in the synthesis ability of 2-PE among different species and strains, and the selection of high-yield 2-PE strains has become the primary condition for biotransformation production

Method used

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Embodiment Construction

[0015] The invention provides a construction of Saccharomyces cerevisiae engineering bacteria producing β-phenylethanol, bacterial strains and applications thereof, specific implementation methods (technical solutions):

[0016] (1) ARO8 and ARO10 genes were amplified from Saccharomyces cerevisiae DNA by PCR technology, connected to shuttle plasmids respectively, and recombinant plasmid expression vectors were constructed, and introduced into S. cerevisiae S288c to overexpress ARO8 and ARO10 genes respectively, and detect their Gene transcription activity, analysis of transaminase, decarboxylase expression level, metabolic flux.

[0017] (2) Connect the ARO8 and ARO10 genes to the shuttle plasmid expression vector at the same time to construct a recombinant plasmid coupling expression vector, and introduce it into S. cerevisiae S288c, screen positive transformants, and overexpress the ARO8 and ARO10 gene coupling to construct a high-yield β- S.cerevisiae engineering bacteria o...

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Abstract

The invention relates to construction of saccharomyces cerevisiae engineering bacteria generating beta-phenethyl alcohol, a strain and application thereof, and belongs to the technical field of bioengineering and production of food spices. The prepared beta-phenethyl alcohol spice is a natural spice and can be used for preparing a plurality of food essences, and therefore, the beta-phenethyl alcohol can be widely applied to industries such as food, medicine and daily use chemicals. Engineering bacterial for coupling overexpression of an aminotransferase gene (ARO8) and decarboxylase gene (ARO10) in the anabolic way of beta-phenethyl are designed and constructed with saccharomycescerevisiae S288 as an original strain; gene copy and expression of the two key enzymes in the main synthetic way are enhanced; the bred engineering bacteria (transformants) are passaged a plurality of times and stable in inheritance; the metabolic flux and the yield of beta-phenethyl are obviously improved. With L-phenylalanine as a major raw material, the engineering bacteria are used for fermenting and converting the raw material into the beta-phenethyl alcohol spice which is single in configuration and chirality and high in fragrance quality, and therefore, a new idea and technical support are provided for preparation of the natural beta-phenethyl alcohol spice.

Description

technical field [0001] The invention relates to the construction, strain and application of Saccharomyces cerevisiae engineering bacteria producing β-phenylethanol, which belongs to the technical field of bioengineering and food flavor manufacturing. The prepared natural β-phenylethanol flavor can be used for the deployment of various food flavors and is widely used In food, medicine, daily chemical and other industries. Background technique [0002] β-phenylethanol (2-phenylethanol, 2-PE), also known as 2-phenylethanol, is a kind of aromatic alcohol with elegant, delicate and long-lasting rose aroma. It has obvious aroma-enhancing effect when added in a small amount. The second largest spice of vanillin, it can be used to blend rose, clove, orange blossom, ylang-ylang, lily of the valley and other fruity flavors and wine flavors, widely used in food, medicine, daily chemical products, cosmetics and tobacco and other industries. 2-PE also has the effects of calming and ref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/54C12N15/60C12N15/81C12P7/22C12R1/865
Inventor 王成涛张婵赵磊孙宝国刘国荣杨雪莲张佳婵
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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