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Preparation and application of biological carbon immobilized microbial agent

A technology of biochar and immobilized microorganisms, applied in biochemical equipment and methods, methods based on microorganisms, immobilized on or in inorganic carriers, etc., can solve the problems of low adsorption capacity, inability to fix carbon and reduce emissions, etc. , to achieve low cost, high oil removal efficiency and high efficiency

Active Publication Date: 2016-11-23
山东百科利生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent document directly uses corn cob as the immobilization carrier without pyrolysis and carbonization of corn cob. The role of row

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Degradation of Petroleum by Pseudomonas

[0036]Pseudomonas sp. was picked and inoculated on beef extract peptone solid medium, and activated for 24 hours. The components of the beef extract peptone solid medium are as follows: 3 g of beef extract, 10 g of peptone, 5 g of NaCl, 10 g of agar, 1000 mL of distilled water, and pH 7.0. Pick Pseudomonas bacteria from beef extract peptone solid medium and transfer them to beef extract peptone liquid medium, culture on a shaker with a rotation speed of 150rpm at 30°C for 18 hours, and then inoculate into crude oil medium according to the inoculation amount of 5% by volume , placed on a shaker at 28°C for 7 days, with a rotation speed of 150rpm. The crude oil culture medium without bacteria was used as blank control. At the end of the cultivation, dichloromethane was added to the culture medium for extraction, and the extract was rotated to evaporate the dichloromethane, and the residual oil content in the culture m...

Embodiment 2

[0039] Embodiment 2: the preparation of biochar

[0040] Corncobs are crushed to less than 2.5cm, dried to a moisture content of ≤10%, and pyrolyzed at 400°C with limited oxygen for 60 minutes to obtain corncob charcoal. Its oil absorption capacity is 6.0g / g and its density is 0.14g / cm 3 , the yield was 30%.

Embodiment 3

[0041] Embodiment 3: the preparation of biochar-immobilized bacterial agent

[0042] Pseudomonas sp. was picked and inoculated on beef extract peptone solid medium, and activated for 24 hours. Pseudomonas bacteria were picked from the beef extract peptone solid medium and transferred to the beef extract peptone liquid medium for seed liquid culture, and cultured on a shaker with a rotation speed of 150 rpm at 30°C for 18 hours. Then inoculate the liquid fermentation medium with an inoculum amount of 5% by volume for cultivation, and culture at 30° C. for 24 hours at a rotation speed of 150 rpm. Pseudomonas bacteria solution was obtained. After testing, the effective number of viable bacteria of Pseudomonas in Pseudomonas bacteria liquid was 6.2×10 7 CFU / mL. The liquid fermentation medium components are as follows: KNO 3 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5 g, anhydrous CaCl 2 0.1g, NH 4 NO 3 1.0g, NaCl 1g, crude oil 2.0g, H 2 O 1000mL. T...

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PUM

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Abstract

The invention relates to preparation and application of a biological carbon immobilized microbial agent. The method comprises the following steps of: sequentially performing seed solution culture and liquid fermentation culture to microorganisms capable of degrading petroleum to obtain fermentation liquid; performing mixed adsorption to the fermentation liquid and biological carbon to prepare the biological carbon immobilized microbial agent. The invention further provides application of the biological carbon immobilized microbial agent to soil polluted by oily sludge and sand and petroleum. By immobilizing the microorganisms having a function of degrading petroleum and then remedying the soil polluted by oily sludge and sand and petroleum, the petroleum removal efficiency is remarkably improved and the removal rate is improved by 20 to 30 percent as compared with remediation by solely using microorganisms. The invention uses agricultural wastes such as corncobs as precursors to prepare carriers, the cost is low, a great amount of microbial agent is easily obtained and simultaneously the reclamation of wastes is realized.

Description

technical field [0001] The invention relates to the preparation and application of a biochar immobilized bacterial agent capable of treating oily mud sand and repairing oil-polluted soil, and belongs to the technical field of environmental bioremediation. Background technique [0002] With the rapid development of the oil industry, the development of oil and gas fields is increasingly polluting the soil. The entry of oil into the soil will destroy the structure and function of the ecosystem and endanger human health. Therefore, it is urgent to restore the contaminated soil. Microbial remediation technology is an efficient, safe and low-cost oil-contaminated soil remediation technology developed by the degradation of microorganisms. However, due to the characteristics of high salinity and poor soil in the onshore oilfields in northern my country, microorganisms are affected by unfavorable conditions. The degradation effect is difficult to fully exert. The immobilized bacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14B09C1/10A62D3/02C12R1/38A62D101/20
CPCA62D3/02A62D2101/20B09C1/10C12N11/14
Inventor 王加宁张闻陈贯虹安德烈·霍赫洛夫傅晓文张强高永超黄玉杰柳德米拉·霍赫洛娃
Owner 山东百科利生态科技有限公司
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